scholarly journals Occurrence of norovirus genogroups I and II in recreational water from four beaches in Belém city, Brazilian Amazon region

2019 ◽  
Vol 17 (3) ◽  
pp. 442-454 ◽  
Author(s):  
Danielle Rodrigues de Deus ◽  
Dielle Monteiro Teixeira ◽  
Jainara Cristina dos Santos Alves ◽  
Vanessa Cavaleiro Smith ◽  
Renato da Silva Bandeira ◽  
...  

Abstract This study aimed to investigate the presence of norovirus (NoV) in recreational waters of four estuarine beaches located in Mosqueiro Island, Belém city, Brazilian Amazon, during two years of monitoring (2012 and 2013). NoV particles were concentrated on filtering membrane by the adsorption-elution method and detected by semi-nested RT-PCR (reverse transcription polymerase chain reaction) and sequencing. NoV positivity was observed in 37.5% (39/104) of the surface water samples, with genogroup GI (69.2%) occurring at a higher frequency than GII (25.7%), with a cocirculation of both genogroups in two samples (5.1%). This virus was detected in all sampling points analyzed, showing the highest detection rate at the Paraíso Beach (46.2%). Statistically, there was a dependence relationship between tide levels and positive detection, with a higher frequency at high tide (46.7%) than at low tide (25%) periods. Months with the highest detection rates (April 2012 and April/May 2013) were preceded by periods of higher precipitation (March 2012 and February/March 2013). Phylogenetic analysis showed the circulation of the old pandemic variant (GII.4-US_95-96) and GI.8. The NoV detection demonstrated viral contamination on the beaches and evidenced the health risk to bathers, mainly through recreational activities such as bathing, and highlighted the importance of including enteric viruses research in the recreational water quality monitoring.

2013 ◽  
Vol 67 (7) ◽  
pp. 1503-1511 ◽  
Author(s):  
Patricia Turgeon ◽  
Pascal Michel ◽  
Patrick Levallois ◽  
André Ravel ◽  
Marie Archambault ◽  
...  

Diverse fecal and nonfecal bacterial contamination and nutrient sources (e.g. agriculture, human activities and wildlife) represent a considerable non-point source load entering natural recreational waters which may adversely affect water quality. Monitoring of natural recreational water microbial quality is most often based mainly on testing a set of microbiological indicators. The cost and labour involved in testing numerous water samples may be significant when a large number of sites must be monitored repetitively over time. In addition to water testing, ongoing monitoring of key environmental factors known to influence microbial contamination may be carried out as an additional component. Monitoring of environmental factors can now be performed using remote sensing technology which represents an increasingly recognized source of rigorous and recurrent data, especially when monitoring over a large or difficult to access territory is needed. To determine whether this technology could be useful in the context of recreational water monitoring, we evaluated a set of agroenvironmental determinants associated with fecal contamination of recreational waters through a multivariable logistic regression model built with data extracted from satellite imagery. We found that variables describing the proportions of land with agricultural and impervious surfaces, as derived from remote sensing observations, were statistically associated (odds ratio, OR = 11 and 5.2, respectively) with a higher level of fecal coliforms in lake waters in the southwestern region of Quebec, Canada. From a technical perspective, remote sensing may provide important added-value in the monitoring of microbial risk from recreational waters and further applications of this technology should be investigated to support public health risk assessments and environmental monitoring programs relating to water quality.


2014 ◽  
Vol 70 ◽  
pp. 545-550 ◽  
Author(s):  
Rosigleyse C. de Sousa ◽  
Luci C.C. Pereira ◽  
Rauquírio M. da Costa ◽  
José A. Jiménez

2021 ◽  
Vol 9 (4) ◽  
pp. 743
Author(s):  
Blenda Gonçalves Cabral ◽  
Danielle Murici Brasiliense ◽  
Ismari Perini Furlaneto ◽  
Yan Corrêa Rodrigues ◽  
Karla Valéria Batista Lima

Surgical site infection (SSI) following caesarean section is associated with increased morbidity, mortality, and significant health care costs. This study evaluated the epidemiological, clinical, and microbiological features of Acinetobacter spp. in women with SSIs who have undergone caesarean section at a referral hospital in the Brazilian Amazon region. This study included 69 women with post-caesarean SSI by Acinetobacter spp. admitted to the hospital between January 2012 and May 2015. The 69 Acinetobacter isolates were subjected to molecular species identification, antimicrobial susceptibility testing, detection of carbapenemase-encoding genes, and genotyping. The main complications of post-caesarean SSI by Acinetobacter were inadequate and prolonged antibiotic therapy, sepsis, prolonged hospitalization, and re-suture procedures. A. baumannii, A. nosocomialis and A. colistiniresistens species were identified among the isolates. Carbapenem resistance was associated with OXA-23-producing A. baumannii isolates and IMP-1-producing A. nosocomialis isolate. Patients with multidrug-resistant A. baumannii infection showed worse clinical courses. Dissemination of persistent epidemic clones was observed, and the main clonal complexes (CC) for A. baumannii were CC231 and CC236 (Oxford scheme) and CC1 and CC15 (Pasteur scheme). This is the first report of a long-term Acinetobacter spp. outbreak in women who underwent caesarean section at a Brazilian hospital. This study demonstrates the impact of multidrug resistance on the clinical course of post-caesarean infections.


2001 ◽  
Vol 17 (suppl) ◽  
pp. S155-S164 ◽  
Author(s):  
Pedro F. C. Vasconcelos ◽  
Amélia P. A. Travassos da Rosa ◽  
Sueli G. Rodrigues ◽  
Elizabeth S. Travassos da Rosa ◽  
Nicolas Dégallier ◽  
...  

A total of 187 different species of arboviruses and other viruses in vertebrates were identified at the Evandro Chagas Institute (IEC) from 1954 to 1998, among more than 10,000 arbovirus strains isolated from humans, hematophagous insects, and wild and sentinel vertebrates. Despite intensive studies in the Brazilian Amazon region, especially in Pará State, very little is known about most of these viruses, except for information on date, time, source, and method of isolation, as well as their capacity to infect laboratory animals. This paper reviews ecological and epidemiological data and analyzes the impact of vector and host population changes on various viruses as a result of profound changes in the natural environment. Deforestation, mining, dam and highway construction, human colonization, and urbanization were the main manmade environmental changes associated with the emergence and/or reemergence of relevant arboviruses, including some known pathogens for humans.


2014 ◽  
Vol 21 (24) ◽  
pp. 13974-13978 ◽  
Author(s):  
Ana Flavia Tonelli Fernandes ◽  
Michelle Barbosa Partata da Silva ◽  
Vinicius Vicente Martins ◽  
Carlos Eduardo Saraiva Miranda ◽  
Eliana Guedes Stehling

1994 ◽  
Vol 44 (3) ◽  
pp. 142-149 ◽  
Author(s):  
João Farias Guerreiro ◽  
Mauro Silvério Figueiredo ◽  
Marco Antonio Zago

2009 ◽  
Vol 69 (2) ◽  
pp. 363-370 ◽  
Author(s):  
SGM. Costa ◽  
RB. Querino ◽  
B. Ronchi-Teles ◽  
AMM. Penteado-Dias ◽  
RA. Zucchi

This study aimed to identify parasitoid species of frugivorous larvae and to describe the tritrophic interactions involving wild fruits, frugivorous insects and their natural enemies at Adolpho Ducke Forest Reserve (RFAD) (Manaus, AM, Brazil). Collections were performed in four 1 km² quadrants in the corners of the RFAD. The wild fruits were collected inside the forest in access trails leading to each collection area and in trails that surrounded the quadrants, up to five metres from the trail on each side. The fruits were placed in plastic containers covered with thin fabric, with a vermiculite layer on the base to allow the emergence of flies or parasitoids. Seven Braconidae species were collected, distributed among Opiinae: Doryctobracon areolatus (Szépligeti, 1911), Utetes anastrephae (Viereck, 1913), and Opius sp., and Alysiinae: Asobara anastrephae (Muesebeck, 1958), Phaenocarpa pericarpa Wharton and Carrejo, 1999, Idiasta delicata Papp, 1969, and Asobara sp. Parasitism rates by braconids and figitids are presented. Doryctobracon areolatus was the most frequent, parasitizing the highest number of fly species, and showing the highest parasitism percentage in larvae feeding on Micropholis williamii fruits. The collected figitids belong to Aganaspis nordlanderi Wharton, 1998 and A. pelleranoi (Brethes, 1924). All 15 tritrophic associations are new records for the Brazilian Amazon region. The RFAD is an important natural reservoir of frugivorous larvae parasitoids.


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