scholarly journals Microbiological analysis of domestic water sources in Banda slum of Kampala, Uganda

Author(s):  
Barugahara Evyline Isingoma ◽  
Kwesiga Stephen

Abstract There is scarcity of information about the safety of water in Banda slum of Kampala, Uganda and yet reports indicate outbreaks of infectious diseases such as typhoid fever and cholera. The aim of this study was to determine the risk of exposure to waterborne infections by Banda residents due to faecal contamination of water sources. Four hundred respondents were sampled and interviewed on the methods of water collection, treatment and storage. Water samples were collected with sterile glass bottles in duplicate from the dug well, protected spring and piped water system in December 2018 on two different consecutive days. They were transported to the laboratory for total and faecal coliform count analysis within 2 h using a lightproof-insulated box containing ice-packs. The mean Escherichia coli count for the dug well was 43 ± 18 c.f.u/mL. The protected spring had no detectable E. coli, but its total plate count level was 76 ± 1.4 c.f.u/mL. Only 46% of the respondents treated their drinking water using boiling and filtering methods. Poor sanitation and hygiene practices were observed. The total and faecal coliform counts of water sources were unsatisfactory making Banda residents highly at risk of infectious diseases, given the small number of residents that treated water.

2020 ◽  
Vol 10 (3) ◽  
pp. 435-446 ◽  
Author(s):  
Sadhana Shrestha ◽  
Yoko Aihara ◽  
Arun P. Bhattarai ◽  
Niranjan Bista ◽  
Naoki Kondo ◽  
...  

Abstract Urban areas in low- and middle-income countries are under chronic water stress, and multiple water source use (MWSU) is common. A detailed study on MWSU is necessary for strengthening water security and enhancing household water resilience to natural disasters which is defined as the ability of a household water system that is exposed to a disaster to resist, accommodate, and recover efficiently in a short time. Surveys were conducted in the Kathmandu Valley, Nepal, before and after the 2015 Gorkha earthquake. A classification of resilient and non-resilient households was based on respondents' perception scores of their water systems before the earthquake and one month after. Around 80% of households used two to three water sources, and 70% of households were classified as water resilient. Three characteristics of a water resilient household were: (i) use of greater number of water sources, (ii) use of multiple reliable water sources such as piped water, groundwater, and (iii) use of effective adaptive strategies such as water storage in a bigger container. Since the study showed the practice of MWSU enhanced the resilience, protection and management of local water sources (well, spring, stone spouts) by initiatives of local government or communities or both is recommended.


2003 ◽  
Vol 47 (3) ◽  
pp. 59-64 ◽  
Author(s):  
C.L. Obi ◽  
N. Potgieter ◽  
P.O. Bessong ◽  
G. Matsaung

The microbial quality of several, usually untreated, surface domestic water sources, used by rural communities in the Venda Region of South Africa, was assessed to gauge their fitness for human consumption and to highlight the possible impact of waterborne diseases. The water sources studied were six points on the Levubu River and the rivers Mutale, Ngwedi, Tshinane, Makonde, Mutshindudi and Mudaswali. Total and faecal coliform, heterotrophic bacteria, enterococci and coliphage counts were used as indicators/surrogates to estimate the degree of bacterial and viral contamination respectively by standard methods. The presence of potential bacterial agents of diarrhoea such as Salmonella, Shigella, Campylobacter, Plesiomonas, Aeromonas and Vibrio was also determined. Results showed that the ranges of counts with regard to all the water sources investigated were 2.9 × 102 - 6.3 × 104 CFU/100 mL for faecal coliforms, 6.0 × 102 - 3.7 × 104 CFU/100 mL for total coliforms, 1.8 × 102 - 1.3 × 106 CFU/mL for heterotrophic plate count, 1.0 × 101 - 3.7 × 104 CFU/100 mL for enterococci and 0-13 PFU/100 mL for coliphages. These values are far higher than the acceptable maximum limits prescribed for South Africa by the Dept of Water & Forestry and the Water Research Commission - 0 CFU/100 mL, 5 CFU/100 mL, 1.0 × 102 CFU/mL, 0 CFU/100 mL and 1 PFU/100 mL for faecal coliforms, total coliforms, heterotrophic bacteria, enterococci and coliphages respectively. Salmonella, Shigella, Vibrio cholerae, Campylobacter, Aeromonas and Plesiomonas were isolated from several of the water sources investigated. The use of these water sources for drinking and domestic purposes poses a serious threat to the health and well being of the users and calls for urgent government intervention.


1970 ◽  
Vol 24 (1) ◽  
pp. 67-69 ◽  
Author(s):  
Monika Das ◽  
Fauzia Hafiz ◽  
M Kawser Ahmed ◽  
Sahana Parveen

Total load of bacteria, fungi and pathogenic microorganisms in muscle-and-skin samples of 12 different species of fish were analyzed. Highest standard plate count (5.1 x 106 cfu/g) was observed in Batashi (Clupisoma atherinoides) and lowest (2.3 x 105 cfu/g) in Rui (Labeo rohita). Total bacterial load was almost similar in case of other fishes. Mold count was highest (7.5 x 102 cfu/g) in shrimp (Penaeus indicus) and lowest (8.0 x 101 cfu/g) in Pabda (Ompok pabda). Yeast count varied between 1.0 x 103 cfu/g in Batashi and 7.0 x 101 cfu/g in Rui. Total coliform and faecal coliform were detected in all the fish samples. Coliform count measured by most probable number (MPN) method was found to be 240/g in all fish samples. Faecal coliform count in the fish samples varied from 2.8 to 240/g. Salmonella was detected in at least two-third of the samples analysed. High microbial load in raw fishes indicates that raw fish would decompose very quickly at ambient temperature, and the presence of coliforms and Salmonella indicates the raw fish handling is not safe.Keywords: Fish, Microbiological analysis, Pathogens Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 67-69


2014 ◽  
Vol 35 (11) ◽  
pp. 1356-1363 ◽  
Author(s):  
Scott Duda ◽  
Sheena Kandiah ◽  
Janet E. Stout ◽  
Julianne L. Baron ◽  
Mohamed Yassin ◽  
...  

Objective.To evaluate the efficacy of a new monochloramine generation system for control ofLegionellain a hospital hot water distribution systemSetting.A 495-bed tertiary care hospital in Pittsburgh, Pennsylvania. The hospital has 12 floors covering approximately 78,000 m2.Methods.The hospital hot water system was monitored for a total of 29 months, including a 5-month baseline sampling period prior to installation of the monochloramine system and 24 months of surveillance after system installation (postdisinfection period). Water samples were collected for microbiological analysis (Legionellaspecies,Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Acinetobacterspecies, nitrifying bacteria, heterotrophic plate count [HPC] bacteria, and nontuberculous mycobacteria). Chemical parameters monitored during the investigation included monochloramine, chlorine (free and total), nitrate, nitrite, total ammonia, copper, silver, lead, and pH.Results.A significant reduction inLegionelladistal site positivity was observed between the pre- and postdisinfection periods, with positivity decreasing from an average of 53% (baseline) to an average of 9% after monochloramine application (P> .05). Although geometric mean HPC concentrations decreased by approximately 2 log colony-forming units per milliliter during monochloramine treatment, we did not observe significant changes in other microbial populations.Conclusions.This is the first evaluation in the United States of a commercially available monochloramine system installed on a hospital hot water system forLegionelladisinfection, and it demonstrated a significant reduction inLegionellacolonization. Significant increases in microbial populations or other negative effects previously associated with monochloramine use in large municipal cold water systems were not observed.Infect Control Hosp Epidemiol2014;35(11):1356–1363


An assessment of domestic water quality using microbial andphysicochemical parameterswas conducted on bothdomestic water supplysourcesand storage containers viz: borehole,upper and lower overhead tanksand stream waterin Calabarmetropolis. Watersamples meant for physicochemical and microbial studies were collected in 1.5L plastic containersandstored in safe conditionprior to laboratory analysis oftheirPhysicochemical and microbial parametersin line withAPHA recommended standardandWHO permissible limits to ascertain if the mean values of the water parameters were or not within the acceptablelimits for human consumption. The results of the descriptive statical analysis of the physicochemical and microbial components of the different domestic water sources revealed mean pH of 6,4±0.6 for the stream water, 6.33±0.57 for the borehole, 5.9±0.9 for the upper overhead tank and 5.79±0.59 for the lower overhead tank. Mean DO concentration of the stream was 7.25±0.25mg/l, with 8.66±0.54mg/lfor the borehole, 6.8±0.2mg/l for the upper overhead tank and 1.79±0.39mg/l for the lower overhead tank (Table 1). Mean water temperature recorded for the stream was 27.5±0.50C, with 27.40±0.60C for the borehole, 27.45±0.550C for the upper overhead tank and 27.71±0.310C for the lower overhead tank. Fe had mean concentration of 1.035±0.001mg/l in the stream water, with 0.040±0.560mg/l in the borehole, 0.664±0.014mg/l in upper overhead tank. Hg mean concentration was <0.001±0.00mg in all the water samples. Bicarbonate had mean concentration of 30.75±0.25mg/l in the stream water, with 36.85±0.256mg/l in the borehole, 24.69±0.31mg/l in the upper overhead tank and 30.75±0.25mg/l in the lower overhead tank. Mean total coliform components were 84.75±0.5 cfu/100ml in the stream water, with 127±2.5 cfu/100ml in the borehole water, 44±2.0 cfu/100ml in the upper overhead tank and 34.5±0.5 cfu/100ml in the lower overhead tank. Total plate count (TPC) had mean values of 127±1,0 cfu/100ml in the stream water, with 164.5±1.5 cfu/100ml in the borehole, 42±8.0 cfu/100ml in the lower overhead tank. Yeast/mould had mean concentration of 0.005±0.005 cfu/100ml in the stream water, with 1.5±0.5 cfu/100ml in the borehole water, 3.5±0.5 cfu/100ml in the upper overhead tank and 2.4±0.4 cfu/100ml in the lower overhead tank. To reducethe risk of human infection that may arise from the continuous use of the water sources andthe tank-stored water, it is strongly recommended that tanks be washed at regular intervals and the treatment of water with recommendeddoses of chemicals meant for such purposes


2019 ◽  
Vol 10 (2) ◽  
pp. 103-109
Author(s):  
Arief Yandra Putra ◽  
Putri Ade Rahma Yulia

Based on a preliminary study that has been carried out, Teluk Nilap Village, Kubu Babussalam District, Rokan Hilir was affected by the disposal of waste from oil company. The waste partly seeps into the residents' water sources which are used for domestic activities. It was because the location of the water sources close to the location of the waste oil disposal. The sampling technique in this study used the observation point technique (pumping test). Sampling refers to the point of waste oil flow (upstream, middle, downstream) and dug well water taken randomly (random sampling). The results showed pH values ranged from 2.8 to 6.6; BOD values range from 0.42 to 141.1 mg / L; COD values range from 20 to 291 mg / L. Ground water quality at some point in Teluk Nilap Village exceeded quality standard limits allowed and included in heavy pollution category.


2021 ◽  
Vol 9 (E) ◽  
pp. 1224-1227
Author(s):  
Mulyadi Mulyadi ◽  
Zaenab Zaenab ◽  
Ain Khaer ◽  
Zrimurti Mappau ◽  
Adriyani Adam

BACKGROUND: Based on the results of laboratory examinations, initial samples taken from one of the clean water sources (dug wells) in Biringere Village, North Sinjai District, results showed that the water sample contained high enough metal ions such as Fe, Mn, hardness, and organic substances which did not meet the requirements as clean water because it still contained levels of Manganese (Mn) 1.68 mg/l. Meanwhile, for organic substances (KMnO4), it is 9876 mg/l. AIM: The purpose of the research was to determine the effectiveness of multimedia filters in improving quality of clean water from the parameters of Mn and KMnO4 in clean water sources (dug wells).. METHODS: The type of research conducted is quasi-experimental by made a filtering processing method with a “Up Flow” flow system, using media consisting of silica sand, zeolite, resin (pine resin), and active carbon in PVC tubes. The population in this study were all dug wells in North Sinjai district. The samples were dug wells located on Biringere Village, North Sinjai District. The samples examined in Center for Environmental Health and disease control of Makassar. RESULTS: The results obtained were that there was a significant change in manganese (Mn) levels of 0.49 mg/l (80.37%). Meanwhile, organic substances (MnO4) amounted to 17.38 mg/l (70.02%). CONCLUSION: Decreasing levels of manganese (Mn) in well water after going through the multimedia filter process above show that the manganese content has met the standard requirements of the Minister of Health Regulation No. 32 of 2017.


2016 ◽  
Vol 20 (2) ◽  
Author(s):  
Sudarmadji Sudarmadji

Groundwater is the main domestic water supply of the population of the Yogyakarta Special Region, both in the urban and as well as in the rural area due to its quantity and quality advantages. The rapid population growth has caused an increase of groundwater demand, consequently it is facing some problems to the sustainability of groundwater supply. Lowering of groundwater level has been observed in some places, as well as the degradation of groundwater quality. Earthquake which stroke Yogyakarta on 27 May 2006, damaged buildings and other infrastructures in the area, including roads and bridges. It might also damage the underground structures such as septic tanks, and pipes underneath the earth surface. It might cause cracking of the geologic structures. Furthermore, the damage of underneath infrastructures might create groundwater quality changes in the area. Some complains of local community on lowering and increasing groundwater level and groundwater quality changes were noted. Field observation and investigation were conducted, including collection of groundwater samples close to (the) pollution sources. Laboratory analyses indicated that some parameters increased to exceed the drinking water quality standards. The high content of Coli form bacteria possibly was caused by contamination of nearby septic tanks or other pollution sources to the observed groundwater in the dug well.


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