Examination of Reactivation and Regrowth of Fecal Coliforms in Centrifuge Dewatered, Anaerobically Digested Sludges

2015 ◽  
Vol 5 (0) ◽  
pp. 9781843397366-9781843397366
Author(s):  
J. H. Matthew
Keyword(s):  
Fecal Coliforms

Physicochemical and bacteriological characterization of hospital effluents and their impact on the environment

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Human Health ◽  
Fecal Coliforms ◽  
Chemical Pollution ◽  
Hospital Effluents ◽  
Biological Contaminants ◽  
Almost All ◽  
Direct Discharge ◽  
Significant Chemical

Liquid effluents discharged by hospitals may contain chemical and biological contaminants whose main source is the different substances used for the treatment of patients. This type of rejection can present a sanitary potentially dangerous risk for human health and can provoke a strong degradation of diverse environmental compartments mainly water and soils. The present study focuses on the quality of the liquid effluents of Hassani Abdelkader’s hospital of Sidi Bel-Abbes (West of Algeria). The results reveal a significant chemical pollution (COD: 879 mgO2/L, BOD5: 850 mgO2/L, NH4+ : 47.9 mg/l, NO2- : 4.2 mg/l, NO3- : 56.8 mg/l with respect to WHO standard of 90 mgO2/L, 30 mgO2/L, 0.5 mg/l, 1 mg/l and 1 mg/l respectively). However, these effluents are biodegradable since the ratio COD/BOD5 do not exceeded the value of 2 in almost all samples. The presence of pathogen germs is put into evidence such as pseudomonas, the clostridium, the staphylococcus, the fecal coliforms and fecal streptococcus. These results show that the direct discharge of these effluents constitutes a major threat to human health and the environment.

Single-stage Sequencing Batch Process for Producing Class A Biosolids from AD Effluent at Terminal Island Treatment Plant

2006 ◽  
Vol 1 (4) ◽  
Author(s):  
Huub H.J. Cox ◽  
Steve Fan ◽  
Reza Iranpour
Keyword(s):  
Fecal Coliform ◽  
Treatment Plant ◽  
Batch Process ◽  
Single Stage ◽  
Fecal Coliforms ◽  
Class A ◽  
Truck Loading ◽  
Train Operation ◽  
Plant Control ◽  
Class A Biosolids

Terminal Island Treatment Plant converted its digesters to thermophilic operation with the objective to comply with the U.S. EPA Part 503 Biosolids Rule requirements for Class A biosolids. The following processes were tested: a) single-stage continuous; b) two-stage continuous; c) single-stage sequencing batch. Salmonella sp. were always non-detect in digester outflows (<3 MPN/4 g dry wt), whereas fecal coliform densities were usually below the Class A limit of 1000 MPN/g dry wt. However, the recurrence of fecal coliforms in post-digestion caused non-compliance with the Class A limit at the truck loading facility as the last point of plant control for compliance. After several design modifications of the post-digestion train, operation of the digesters as sequencing batch digesters according to the time-temperature requirement of Alternative 1 of the Part 503 Biosolids Rule achieved compliance for both Salmonella sp. and fecal coliforms at the last point of plant control (truck loading facility).

Campylobacter in waterborne epidemics in Finland

2004 ◽  
Vol 4 (2) ◽  
pp. 39-45 ◽  
Author(s):  
M.-L. Hänninen ◽  
R. Kärenlampi
Keyword(s):  
Campylobacter Jejuni ◽  
Causative Agent ◽  
Water Samples ◽  
Fecal Coliforms ◽  
Risk Level ◽  
Source Water ◽  
Indicator Organisms ◽  
Faecal Contamination ◽  
E Coli ◽  
July August September

The sources for drinking water in Finland are surface water, groundwater or artificially recharged groundwater. There are approximately 1400 groundwater plants in Finland that are microbiologically at a high risk level because in most cases they do not use any disinfection treatment. Campylobacter jejuni has caused waterborne epidemics in several countries. Since the middle of the 1980s, C. jejuni has been identified as the causative agent in several waterborne outbreaks in Finland. Between 1998 and 2001, C. jejuni or C. upsaliensis caused seven reported waterborne epidemics. In these epidemics approximately 4000 people acquired the illness. Most of the outbreaks occurred in July, August , September or October. In four of them source water and net water samples were analysed for total coliforms or fecal coliforms, E. coli and campylobacters. We showed that large volumes of water samples in studies of indicator organisms (up to 5000 ml) and campylobacters (4000–20,000 ml) increased the possibility to identify faecal contamination and to detect the causative agent from suspected sources.

Use in Agriculture of Sludge from Chemical Treatment of Sewage

1987 ◽  
Vol 19 (8) ◽  
pp. 139-145
Author(s):  
G. Castillo ◽  
A. Ortega
Keyword(s):  
Sodium Hydroxide ◽  
Chemical Treatment ◽  
Application Rate ◽  
Additional Treatment ◽  
Fecal Coliforms ◽  
Aluminium Sulfate ◽  
Leguminous Crops ◽  
Dried Sludge ◽  
Chemical Sludge ◽  
Sludge Application

The reutilization in agriculture of the sludge produced in the chemical treatment of sewage is investigated. Aluminium sulfate (50 mg/l) and sodium hydroxide (50 and 200 mg/l) are added to domestic sewage allowing it to settle and eliminating floating materials. Three kinds of raw chemical sludge are digested and dried. Their sanitary quality is determined by two bacterial indicators (total and fecal coliforms) and one viral indicator (E. coli bacteriophage) and the fertilizing capability of digested and dry sludge by sowing leguminous crops (Phaseolus spp ) in soil with 11 tons/ha sludge application rate. Digested and dried sludge from conventional treatment in equal conditions to those of chemical sludge is used as reference. Sanitary quality results show that raw and digested chemical sludge are not recommended for use in agriculture due to their high microbiological contamination. Dry sludge could be considered for this purpose due to its low fecal organisms content. However the development of leguminous crops in soil with dry aluminium sludge application shows no seed germination. The results of leguminous growth in digested sludge of sodium hydroxide (200 mg/l) justify an additional treatment to adequate its sanitary quality for use in agriculture.

Water Quality of Swimming Pools in Jerusalem

1993 ◽  
Vol 27 (7-8) ◽  
pp. 287-294 ◽  
Author(s):  
S. Lerman ◽  
O. Lev ◽  
A. Adin ◽  
E. Katzenelson
Keyword(s):  
Water Quality ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Swimming Pools ◽  
Indicator Organisms ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Safe Water ◽  
Indirect Information

The Israel Ministry of Health is now revising its regulations for the assurance of safe water quality in public swimming pools. Since it is not possible to monitor each of the pathogenic microorganisms, it is often recommended to monitor indicator bacteria which provide indirect information on the water quality in the swimming pool. Three indicator microorganisms are often recommended: coliform counts (total coliforms, fecal coliforms or E. Coli), staphylococcus aureus and pseudomonas aeruginosa. A four year survey of the water quality of swimming pools in the Jerusalem District was conducted in order to determine whether the monitoring of all three indicators is necessary to assure safe water quality or is it sufficient to monitor only a single microorganism. A statistical analysis, conducted by using several different statistical techniques, reveals that the populations of the three indicator organisms are significantly interdependent but the correlations between each pair of these indicators are not sufficient to base a prediction of any of the organisms based on the measurements of the others. Therefore, it is concluded that monitoring of all three indicators should be recommended in order to provide an adequate picture of the water quality in swimming pools.

Wastewater Treatment by Stabilization Ponds with and without Macrophytes under Arid Climate

1993 ◽  
Vol 28 (10) ◽  
pp. 177-181 ◽  
Author(s):  
L. Mandi ◽  
N. Ouazzani ◽  
K. Bouhoum ◽  
A. Boussaid
Keyword(s):  
Bacterial Load ◽  
Fecal Coliforms ◽  
Arid Climate ◽  
Organic Load ◽  
Experimental Systems ◽  
Helminth Eggs ◽  
Stabilization Ponds ◽  
Treated Effluents ◽  
Summer Time ◽  
Influent Flow

This paper presents a comparative study of two experimental systems, stabilization ponds without macrophytes and macrophytic ponds, to purify wastewater under arid climate of Marrakesh. Organic load (COD, TSS), bacterial load (SF, CF) and parasitical load (helminth eggs) are significantly reduced in both systems. Even though water loss was more important at the macrophytic ponds (evapotranspiration reaches 60% of influent flow), this system shows generally the best efficiency in purifying wastewater. In summer time, TSS and COD depletion is better on macrophytic ponds (TSS: 95%, COD: 87%). The stabilization ponds are more efficient to reduce nutrients: NH4+: 72%. PO4: 63% at the same period. Fecal streptococci and fecal coliforms are reduced more in stabilization ponds (CF: 99.4%, SF: 99.7%) than macrophytic ponds (CF: 96.2%, SF: 94.7%). No helminth eggs were found in the effluent from either system. The two systems ensure optimal removal of parasites allowing the eventual reuse of the treated effluents for agricultural purposes.

Microbiological performance and salmonella dynamics in a wastewater depuration pond system of southeastern spain

1995 ◽  
Vol 31 (12) ◽  
pp. 239-248 ◽  
Author(s):  
Ana Emparanza-Knörr ◽  
Francisco Torrella
Keyword(s):  
Fecal Coliform ◽  
No Reduction ◽  
Agar Medium ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Selective Media ◽  
E Coli ◽  
The Village ◽  
Final Effluent

The Salmonella presence and the microbiological quality indicators, total and fecal coliforms and coliphages of E. coli C, have been studied in a overloaded wastewater lagoon system treating urban wastewatrers of the village of Guardamar del Segura (Alicante, Spain). Classical microbiological technology to detect salmonellae was used, including pre-enrichment, enrichment, selective media plating and biochemical and serological confirmation. Water was physicochemically characterized according to COD, SS, temperature, pH and dissolved oxygen. The selective migration step through Rappaport-Vassiliadis semisolid agar medium was essential for the consistent detection of Salmonella in the different lagoon effluents. Total and fecal coliform levels of up to 105-106 MPN/100 ml were detected in the final effluent. High coliphage concentrations of 103-104 pfu/ml were also found in the effluent waters. Salmonella was always detected in 100 ml samples and eventually reached an order of value of 103 MPN/100 ml. Total coliform reduction was higher in the anaerobic ponds whereas fecal coliforms were more efficiently eliminated in the facultative (mostly “anoxic”) lagoons. Coliphage reduction was higher in the facultative lagoons when compared to the anaerobic ponds. On many occasions, no reduction or eventual increment of the concentration of salmonellae was detected in the effluents from the anaerobic ponds compared to concentrations of the patohogen in the influent raw wasterwaters. The possibility exists for a capacity of Salmonella to multiply in the anoxic phase of the wastewater treatment, but the presence of microorganisms in raw sewage waters which could maskSalmonella detection with the enrichment methodology employed cannot be ruled out.

Optimization of Artificial Wetland Design for Removal of Indicator Microorganisms and Pathogenic Protozoa

1999 ◽  
Vol 40 (4-5) ◽  
pp. 363-368 ◽  
Author(s):  
C. P. Gerba ◽  
J. A. Thurston ◽  
J. A. Falabi ◽  
P. M. Watt ◽  
M. M. Karpiscak
Keyword(s):  
Subsurface Flow ◽  
Individual Performance ◽  
Surface Flow ◽  
Enteric Pathogens ◽  
Fecal Coliforms ◽  
Treatment Level ◽  
Artificial Wetland ◽  
Indicator Microorganisms ◽  
Duckweed Pond ◽  
Wetland Design

The enhancement of water quality by artificial wetland systems is increasingly being employed throughout the world. Three wetlands were studied in Tucson, AZ to evaluate their individual performance in the removal of indicator bacteria (coliforms), coliphage, and enteric pathogens (Giardia and Cryptosporidium). A duckweed-covered pond, a multi-species subsurface flow (SSF) and a multi-species surface flow (SF) wetland were studied. Removal of the larger microorganisms, Giardia and Cryptosporidium, was the greatest in the duckweed pond at 98 and 89 percent, respectively. The lowest removal occurred in the SF wetland, 73 percent for Giardia and 58 percent removal for Cryptosporidium. In contrast, the greatest removal of coliphage, total and fecal coliforms occurred in the SSF wetland, 95, 99, and 98 percent respectively, whereas the pond had the lowest removals (40, 62, and 61 percent, respectively). Sedimentation may be the primary removal mechanism within the duckweed pond since the removal was related to size, removal of the largest organisms being the greatest. However, the smaller microorganisms were removed more efficiently in the SSF wetland, which may be related to the large surface area available for adsorption and filtration. This study suggests that in order to achieve the highest treatment level of secondary unchlorinated wastewater, a combination of aquatic ponds and subsurface flow wetlands may be necessary.

Behaviour of the fecal pollution indicators in a soil irrigated with treated wastewater under onsurface and subsurface drip irrigation

2000 ◽  
Vol 42 (1-2) ◽  
pp. 75-79 ◽  
Author(s):  
C. Campos ◽  
G. Oron ◽  
M. Salgot ◽  
L. Gillerman
Keyword(s):  
Organic Matter ◽  
Drip Irrigation ◽  
Organic Matter Content ◽  
Fecal Pollution ◽  
Fecal Coliforms ◽  
Subsurface Drip Irrigation ◽  
Treated Wastewater ◽  
Reclaimed Wastewater ◽  
Pollution Indicators ◽  
Subsurface Drip

A critical objective for any wastewater reuse programme is to minimise health and environmental hazard. When applying wastewater to soil–plant systems, it is to be noted that the passage of water through the soil considerably reduces the number of microorganisms carried by the reclaimed wastewater. Factors that affect survival include number and type of microorganisms, soil organic matter content, temperature, moisture, pH, rainfall, sunlight, protection provided by foliage and antagonism by soil microflora. The purpose of this work was to examine the behaviour of fecal pollution indicators in a soil irrigated with treated wastewater under onsurface and subsurface drip irrigation. The experiment was conducted in a vineyard located at a commercial farm near the City of Arad (Israel). Wastewater and soil samples were monitored during the irrigation period and examined for fecal coliforms, somatic and F+ coliphages and helminth eggs. Physico-chemical parameters were controlled in order to determine their relationship with removal of microorganisms. The results showed high reduction of the concentration of microorganisms when wastewater moves through the soil; and a good correlation between the reduction of fecal pollution indicators and moisture content, organic matter concentration and pH. The application of secondary treated domestic wastewater in this specific soil and under these irrigation systems affect the survival of microorganisms, thus reducing the health and environmental risk.

scholarly journals Ultrafiltration Process in Disinfection and Advanced Treatment of Tertiary Treated Wastewater

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 221
Author(s):  
Rafał Tytus Bray ◽  
Katarzyna Jankowska ◽  
Eliza Kulbat ◽  
Aneta Łuczkiewicz ◽  
Aleksandra Sokołowska
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Microscopic Analysis ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
Chemical Parameters ◽  
Virus Particles ◽  
E Coli ◽  
The One ◽  
Physical And Chemical

The paper presents the results of research on the use of ultrafiltration, using membranes of 200 and 400 kDa separation, for disinfection of municipal treated wastewater. The research was conducted on a fractional technical scale using real municipal treated wastewater from two large wastewater treatment plants treating most of the wastewater over the one-million polycentric Gdańsk agglomeration (1.2 million inhabitants). UF 200 kDa and UF 400 kDa processes enabled further improvement of the physical and chemical parameters of treated wastewater. Total phosphorus (to below 0.2 mg/L–UF 200 kDa, 0.13 mg/L–UF 400 kDa) and turbid substances (to below 0.2 mg/L, both membranes) were removed in the highest degree. COD was reduced efficiently (to below 25.6 mgO2/L–UF 200 kDa, 26.8 mgO2/L–UF 400 kDa), while total nitrogen was removed to a small extent (to 7.12 mg/L–UF 200 kDa and 5.7 mg/L–UF 400 kDa. Based on the reduction of indicator bacteria; fecal coliforms including E. coli (FC) and fecal enterococci (FE) it was found that the ultrafiltration is an effective method of disinfection. Not much indicator bacterial were observed in the permeate after processes (UF 200 kDa; FC—5 CFU/L; FE—1 CFU/L and UF 400 kDa; FC—70 CFU/L; FE—10 CFU/L. However, microscopic analysis of prokaryotic cells and virus particles showed their presence after the application of both membrane types; TCN 3.0 × 102 cells/mL–UF 200 kDa, 5.0 × 103 cells/mL–UF 400 kDa, VP 1.0 × 105/mL. The presence of potentially pathogenic, highly infectious virus particles means that ultrafiltration cannot be considered a sufficient disinfection method for treated wastewater diverted for reuse or discharged from high load wastewater treatment plants to recreational areas. For full microbiological safety it would be advisable to apply an additional disinfection method (e.g., ozonation).

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