Molecular Detection of Antibiotic Resistance Genes in E.Coli Isolated From Poultry Meat

2017 ◽  
Vol 22 (2) ◽  
pp. 235-246
Author(s):  
Ahmed Khafagy ◽  
F. Youssef ◽  
A. Aly
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Molecular Detection ◽  
Antibiotic Resistance Genes ◽  
Poultry Meat

scholarly journals  Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran

2012 ◽  
Vol 57 (No. 4) ◽  
pp. 193-197 ◽  
Author(s):  
H. Momtaz ◽  
E. Rahimi ◽  
S. Moshkelani
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Molecular Detection ◽  
Antimicrobial Agents ◽  
Antibiotic Resistance Genes ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Antimicrobial Resistance Genes ◽  
Meat Samples ◽  
Commercial Chickens

This study was carried out to detect the distribution of antibiotic-resistant genes in Escherichia coli isolates from slaughtered commercial chickens in Iran by PCR. The investigated genes included aadA1, tet(A), tet(B), dfrA1, qnrA, aac(3)-IV, sul1, bla<sub>SHV</sub>, bla<sub>CMY</sub>, ere(A), catA1 and cmlA. According to biochemical experiments, 57 isolates from 360 chicken meat samples were recognized as E. coli. The distribution of antibiotic-resistance genes in the E. coli isolates included tet(A) and tet(B) (52.63%), dfrA1, qnrA, catA1 and cmlA (36.84%) and sul1 and ere(A) (47.36%), respectively. Nine strains (15.78%) were resistant to a single antimicrobial agent and 11 strains (19.29%) showed resistance to two antimicrobial agents. Multi-resistance which was defined as resistance to three or more tested agents was found in 64.91% of E. coli strains. The results indicate that all isolates harbour one or more of antibiotic resistance genes and that the PCR technique is a fast, practical and appropriate method for determining the presence of antibiotic-resistance genes. &nbsp;

Molecular detection of antibiotic resistance genes, and class I, and II integrons in Salmonella enteritidis isolated from Iranian one-day-old chicks

Gene Reports ◽  
2019 ◽  
Vol 16 ◽  
pp. 100441
Author(s):  
Mehran Ghazalibina ◽  
Reza Khaltabadi Farahani ◽  
Shamseddin Mansouri ◽  
Maryam Meskini ◽  
Amir Hossien Khaltabadi Farahani ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Salmonella Enteritidis ◽  
Molecular Detection ◽  
Antibiotic Resistance Genes ◽  
Class I

Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures

2014 ◽  
pp. 97-108 ◽  
Author(s):  
Musa Y. Hindiyeh ◽  
Gill Smollan ◽  
Shiraz Gefen-Halevi ◽  
Ella Mendelson ◽  
Nathan Keller
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Molecular Detection ◽  
Antibiotic Resistance Genes ◽  
Blood Cultures

Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

2016 ◽  
Vol 1 (2) ◽  
pp. 22 ◽  
Author(s):  
Navindra Kumari Palanisamy ◽  
Parasakthi Navaratnam ◽  
Shamala Devi Sekaran
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Pcr Amplification ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Efflux Mechanism ◽  
Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

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