Microorganismsare responsible for the progress and persistence of inflammatory response of pulpal and periapical tissues have been well recognized. Endodontic treatment success depends mainly on the removal of microbial contamination from infected root canal. On one hand, bacterial count can be decreased by mechanical instrumentation of root canals, but on the other handcomplete removal of microorganism cannot be obtained without the usingintra canal irrigants and medicaments. This review will t

2018 ◽  
Vol 5 (19) ◽  
pp. 10-16
2020 ◽  
Vol 75 (10) ◽  
pp. 553-563
Author(s):  
Maheshan Pillay ◽  
Martin Vorster ◽  
Peet J Van der Vyver

Instrument fracture is a serious complication during endodontic treatment of teeth, having an adverse effect on the outcome of the nickel titanium (NiTi) treatment, especially if the fracture prevents apical access to the infected root canal. Despite the advent of NiTi files, the risk of fracture during the endodontic preparation of root canals, especially in severely curved canals, remains a serious concern. The fracture of NiTi files during preparation may result in a compromised prognosis for the tooth. In the presence of periapical lesions, instrument fracture may reduce the chances of successful healing.


2005 ◽  
Vol 5 (4) ◽  
pp. 35-39 ◽  
Author(s):  
Amela Lačević ◽  
Nurija Bilalović ◽  
Aida Kapić

The aim of this study was to investigate different microbial morphotypes in the root canal infection associated with chronic diffuse periapical lesion. In forty cases of asymptomatic teeth with radiographically diagnosed diffuse periapical lesion we took specimens of infected tissue from the root canals at the beginning of endodontic treatment. Fixation and four different staining methods of the specimens were obtained to provide microscope examination. All examined root canal specimens were heavily infected by bacteria. The most commonly identified were cocci 92 %, small mostly G+ diplococci and large G+cocci in clusters and grapelike groups, bacilli found in 67%, coccobacilli 37%, fungi 17%, and spirochetes in 5%.


2010 ◽  
Vol 9 (1) ◽  
pp. 63
Author(s):  
Indrya Kirana Mattulada

Bacteria and their products play a fundamental role in the initiation, propagation andpersistence of pulpal and periradicular diseases. Consequently one of the major goalsof endodontic treament is to eliminate all bacteria from the root canal space.Elimination of microorganism from infected root canal is a complicated task.Numerous measures have been described to reduce the numbers of root canalmicroorganism, including the use of various instrumentation techniques, irrigationregimens, and intracanal medication. In endodontic treatment, there are severalmedicament used traditionally. They are phenolic, aldehyde, halogen, calciumhydroxide, and antibiotic. The aim of this paper is to discuss about indication,contraindication and effect of each medicament.


2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Sheela B. Abraham ◽  
Farah al Marzooq ◽  
Wan Harun Himratul-Aznita ◽  
Hany Mohamed Aly Ahmed ◽  
Lakshman Perera Samaranayake

Abstract Background There is limited data on the prevalence of Candida species in infected root canal systems of human teeth. We attempted to investigate the prevalence, genotype, virulence and the antifungal susceptibility of Candida albicans isolated from infected root canals of patients with primary and post-treatment infections in a UAE population. Methods Microbiological samples from 71 subjects with infected root canals were aseptically collected, and cultured on Sabouraud dextrose agar, and C. albicans was identified using multiplex polymerase chain reaction, and the isolates were further subtyped using ABC genotyping system. Their relative virulence was compared using further four archival samples of endodontic origin from another geographical region, and four more salivary isolates, as controls. The virulence attributes compared were biofilm formation, and production of phospholipase and haemolysin, and the susceptibility to nystatin, amphotericin B, ketoconazole, and fluoconazole was also tested. Results 4 out of 71 samples (5.6%) yielded Candida species. On analysis of variance among the groups, the intracanal isolates, mainly Genotype A, possessed a high degree of phospholipase and haemolysin activity (p < 0.05). The UAE and Finland isolates were stronger biofilm formers and had superior phospholipase production capacity compared with the salivary isolates. All isolates were sensitive to the antifungal chemicals used. The salivary isolates were more sensitive to fluoconazole compared to other groups (p < 0.05). Conclusion The prevalence of C. albicans in infected root canals of patients attending a dental hospital in UAE is low. The strains isolated were good biofilm formers, possessed strong phospholipase and haemolysin activity and were mostly of the genotype A. The fact that the root canal isolates possessed significant hydrolase activity, imply that they are equipped with virulence attributes necessary for survival within a harsh intracanal ecosystem.


Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7594
Author(s):  
Zhejun Wang ◽  
Ya Shen ◽  
Markus Haapasalo

Microbes are prevalent in the root canals of necrotic teeth, and they are the cause of primary and post-treatment apical periodontitis. Bacteria can dwell within the infected root canal system as surface-adherent biofilm structures, which exhibit high resistance to antimicrobial agents. Bioceramic materials, with their biocompatible nature and excellent physico-chemical properties, have been widely used in dental applications, including endodontics. This review focuses on the application of bioceramic technology in endodontic disinfection and the antibiofilm effects of endodontic bioceramic materials. Different bioceramic materials have shown different levels of antibiofilm effects. New supplements have emerged to potentially enhance the antibiofilm properties of bioceramics aiming to achieve the goal of microbial elimination in the root canal system.


2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Takuichi Sato ◽  
Keiko Yamaki ◽  
Naoko Ishida ◽  
Megumi Shoji ◽  
Emika Sato ◽  
...  

Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment.Methods. Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23–79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4′,6′-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer.Results. The mean ± SD of CFU in the sample of “#25 First” was(1.0±1.4)×105. As the root canal treatment progressed, the CFU decreased as7.9×103(#55 First) and4.3×102(#55 Second).Conclusion. In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).


Author(s):  
Aashima Puri ◽  
Jyoti Nagpal ◽  
Mayank Mall ◽  
Pinki Narwal ◽  
Deepti Garg ◽  
...  

Biofilm can be defined as a sessile multi-cellular microbial community characterized by cells that are firmly attached to a surface and enmeshed in a self produced matrix of extracellular polymeric substances.1,2 These are very prevalent in the apical root canals of teeth with primary and post-treatment apical periodontitis.3 It can be categorised as intracanal biofilms, extra radicular biofilms, periapical biofilms and biomaterial centered infections.4 The infected root canal harbors a polymicrobial population of aerobic, anaerobic, Gram-positive, and Gram-negative bacteria in a biofilm mode of growth. Bacterias involved are E. faecalis, Coagulase–negative Staphylococcus, S. aureus, Streptococci, P.aeruginosa, fungi, F. nucleatum, P. gingivalis, T. forsythensis, Actinomyces species and P. Propionicum. These are commonly isolated by culture, microscopy, immunological methods and molecular biology methods.3 The microbial communities grown in biofilm are remarkably difficult to eradicate with antimicrobial agents. Therefore, different antimicrobials ranging from antimicrobial irrigants to advanced antimicrobial methods such as lasers, photoactivated disinfection, and nanoparticles are employed in the management of infected root canal systems.4


Author(s):  
Pâmela de Medeiros Dantas ◽  
◽  
Renata Correia Sotero Dália Torres ◽  
Robeci Alves Macedo Filho ◽  
Sandra Aparecida Marinho ◽  
...  

Introduction: Endodontic instruments fracture is related to several factors from internal dental anatomy and type, to operator inability and inexperience. Objective: To review case reports related to fracture of endodontic instruments inside the root canal. Methodology: A literature review was carried out using papers available in the PubMed and Lilacs databases. Only case reports were included, with no restriction on language and publication year Results: It was analyzed eight papers with 11 instrument fracture cases, with the highest prevalence (45.5%) of rotary instruments fracture, occurring mainly in middle-cervical and middle-apical root canals thirds. In 90.9% of cases, techniques used for removal showed successful results and in only one case (9.1%), the technique was unsuccessful and the instrument was kept inside the root canal. Final considerations: Endodontic instruments fracture is a complication can directly affect prognosis and endodontic treatment success. Several techniques and devices can be used to remove fractured fragments inside root canals, however, there is no specific protocol for their removal. Keywords: accidents; endodontics; fracture; dental instruments; prognosis.


2021 ◽  
pp. 232020682110189
Author(s):  
Ramesh Bharti ◽  
Aseem Prakash Tikku ◽  
Anil Chandra ◽  
Prashant Gupta

Objective: To evaluate the effectiveness of photodynamic therapy (PDT), sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHO) in the endodontic re-treatment in the removing of microorganisms from previously filled infected root canals. Materials and Methods: The study samples consist of 54 root canal treated anterior teeth with apical periodontitis were randomly divided into three groups according to the disinfection protocol. Microbiological samples from the root canals were collected after removing the gutta-percha (S1) and following the endodontic re-treatment (S2) with either 5% NaOCl, 2% CHO or PDT procedure for 1 minute. Microbiological samples were cultivated on selective plates to assess the presence or absence of microbial growth and determine the average reduction of viable microorganisms. Results: Significant reduction in the total bacterial count in all the groups at post treatment ( P < .001). PDT provided statistically higher (99.74%) elimination of Enterococcus faecalis in comparison to NaOCl (95%) and CHO (88%). Conclusions: The use of PDT in endodontic re-treatment led to the significant reduction of the remaining bacterial species. So, PDT can be used for routine endodontic disinfection in failed root canal cases.


2020 ◽  
Vol 1 (12) ◽  
pp. 40-42
Author(s):  
F. Yu. Daurova ◽  
D. I. Tomaeva ◽  
S. V. Podkopaeva ◽  
Yu. A. Taptun

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.


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