scholarly journals Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231

2017 ◽  
Vol 2 (1) ◽  
pp. 26-39 ◽  
Author(s):  
Sheir DH ◽  
Hafez MA
Keyword(s):  
Candida Albicans ◽  
Antibiofilm Activity ◽  
Streptomyces Toxytricini

scholarly journals A Label-Free Cellular Proteomics Approach to Decipher the Antifungal Action of DiMIQ, a Potent Indolo[2,3-b]Quinoline Agent, against Candida albicans Biofilms

2020 ◽  
Vol 22 (1) ◽  
pp. 108
Author(s):  
Robert Zarnowski ◽  
Anna Jaromin ◽  
Agnieszka Zagórska ◽  
Eddie G. Dominguez ◽  
Katarzyna Sidoryk ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Candida Albicans ◽  
Ribosomal Proteins ◽  
Nucleotide Metabolism ◽  
Antibiofilm Activity ◽  
Label Free ◽  
Xtt Assay ◽  
Fungal Cell ◽  
Select Amino Acid ◽  
Cellular Oxidation

Candida albicans forms extremely drug-resistant biofilms, which present a serious threat to public health globally. Biofilm-based infections are difficult to treat due to the lack of efficient antifungal therapeutics, resulting in an urgent demand for the development of novel antibiofilm strategies. In this study, the antibiofilm activity of DiMIQ (5,11-dimethyl-5H-indolo[2,3-b]quinoline) was evaluated against C. albicans biofilms. DiMIQ is a synthetic derivative of indoquinoline alkaloid neocryptolepine isolated from a medicinal African plant, Cryptolepis sanguinolenta. Antifungal activity of DiMIQ was determined using the XTT assay, followed by cell wall and extracellular matrix profiling and cellular proteomes. Here, we demonstrated that DiMIQ inhibited C. albicans biofilm formation and altered fungal cell walls and the extracellular matrix. Cellular proteomics revealed inhibitory action against numerous translation-involved ribosomal proteins, enzymes involved in general energy producing processes and select amino acid metabolic pathways including alanine, aspartate, glutamate, valine, leucine and isoleucine. DiMIQ also stimulated pathways of cellular oxidation, metabolism of carbohydrates, amino acids (glycine, serine, threonine, arginine, phenylalanine, tyrosine, tryptophan) and nucleic acids (aminoacyl-tRNA biosynthesis, RNA transport, nucleotide metabolism). Our findings suggest that DiMIQ inhibits C. albicans biofilms by arresting translation and multidirectional pathway reshaping of cellular metabolism. Overall, this agent may provide a potent alternative to treating biofilm-associated Candida infections.

scholarly journals Potentiation of Antibiofilm Activity of Amphotericin B by Superoxide Dismutase Inhibition

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Katrijn De Brucker ◽  
Anna Bink ◽  
Els Meert ◽  
Bruno P. A. Cammue ◽  
Karin Thevissen
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Superoxide Dismutases ◽  
Antibiofilm Activity ◽  
Oxygen Species ◽  
Ammonium Tetrathiomolybdate

This study demonstrates a role for superoxide dismutases (Sods) in governing tolerance ofCandida albicansbiofilms to amphotericin B (AmB). Coincubation ofC. albicansbiofilms with AmB and the Sod inhibitors N,N′-diethyldithiocarbamate (DDC) or ammonium tetrathiomolybdate (ATM) resulted in reduced viable biofilm cells and increased intracellular reactive oxygen species levels as compared to incubation of biofilm cells with AmB, DDC, or ATM alone. Hence, Sod inhibitors can be used to potentiate the activity of AmB againstC. albicansbiofilms.

scholarly journals Identification of a new antifungal oligoacetal derivative produced by Streptomyces toxytricini against Candida albicans

2015 ◽  
Vol 30 (16) ◽  
pp. 1816-1823 ◽  
Author(s):  
Ahmed Z. Abdel Azeiz ◽  
Donia K. Hanafi ◽  
Sameh E. Hasanein
Keyword(s):  
Candida Albicans ◽  
Streptomyces Toxytricini

scholarly journals Antimicrobial Activity of Non-bond Colicin on Candida albicans Biofilm

2020 ◽  
pp. 2831-2837
Author(s):  
Nayarah .S. Hussain ◽  
Ismail .I. Latif ◽  
Hind .H. Obaid
Keyword(s):  
Candida Albicans ◽  
Teaching Hospital ◽  
Inhibition Zone ◽  
Significant Inhibition ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Study Results ◽  
Lowry Method ◽  
Tract Infections ◽  
Urine Specimens

Two hundred fifty mid-stream urine specimens were collected from Baqubah Teaching Hospital and Al-Batool Teaching Hospital from patients with urinary tract infections (UTI). Of these investigated urine specimens, 66 (26.4%) specimens showed positive growth culture of Gram-negative bacteria. From these, Escherichia coli was the most prevalent bacteria of the examined culture (41, 62.12%). Additionally, the cup assay was used to determine colicin producers while the most efficient colicin producers were estimated by the formation of larger inhibition zone. Approximately half of the investigated E. coli isolates (20, 49 %) was colicin producers. Colicins was extracted after induction by mitomycin-C showed a concentration of 3020 μg/ml,  as estimated utilizing the Lowry method, while its activity was 80 U/ml. Our study results showed that colicin had significant antibiofilm activity (P≤ 0.05) against Candida albicans and the effect seemed to be concentration dependent. . However, the values of biofilm inhibition varied depending on the different tested isolates. The biofilm of isolate 5 showed the most significant inhibition (P≤ 0.05) by colicin with a value of 46%, while isolate 3 was less affected with an inhibition rate of 19% at the concentration of 2500 μl/ml.

scholarly journals Candida albicans Antimicrobial and Antibiofilm Activity of Novel Endodontic Solvents

2021 ◽  
Vol 11 (16) ◽  
pp. 7748
Author(s):  
Inês Ferreira ◽  
Maria Elisa Rodrigues ◽  
Liliana Fernandes ◽  
Mariana Henriques ◽  
Irene Pina-Vaz
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Treatment ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Solvent Mixtures ◽  
The Novel ◽  
Disk Diffusion Method ◽  
Colony Forming Units ◽  
Endodontic Infections ◽  
Additional Exposure

Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures’ exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol.

Zerumbone inhibits Candida albicans biofilm formation and hyphal growth

2019 ◽  
Vol 65 (10) ◽  
pp. 713-721 ◽  
Author(s):  
Da-Seul Shin ◽  
Yong-Bin Eom
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Antifungal Agents ◽  
Hyphal Growth ◽  
Antibiofilm Activity ◽  
Potential Candidate ◽  
Zingiber Zerumbet ◽  
Dna Contents ◽  
Important Virulence Factor ◽  
Morphologic Changes

Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a monocyclic sesquiterpene extracted from Zingiber zerumbet (L.) Smith, against C. albicans biofilm formation. Our results suggest that zerumbone possesses antifungal and antibiofilm activity that inhibits biofilm formation and eradicates preformed biofilm. Notably, zerumbone considerably reduced carbohydrate and DNA contents of biofilm matrix. In addition, zerumbone showed antivirulence effects by decreasing the growth of hyphae and inhibiting morphologic changes of C. albicans. Furthermore, zerumbone significantly downregulated expression levels of biofilm-related and hyphae-specific genes, including HWP1 and ALS3. Since zerumbone suppresses biofilm formation and hyphae growth, these results indicate that zerumbone could be used as a potential candidate to treat and prevent C. albicans biofilm-related infections.

scholarly journals In Vitro Antibiofilm Activity of Eucarobustol E against Candida albicans

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Rui-Huan Liu ◽  
Zhi-Chun Shang ◽  
Tian-Xiao Li ◽  
Ming-Hua Yang ◽  
Ling-Yi Kong
Keyword(s):  
Candida Albicans ◽  
Antifungal Susceptibility ◽  
Hyphal Growth ◽  
Negative Regulator ◽  
Ergosterol Biosynthesis ◽  
Pcr Analysis ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Content Type

ABSTRACT Formyl-phloroglucinol meroterpenoids (FPMs) are important types of natural products with various bioactivities. Our antifungal susceptibility assay showed that one of the Eucalyptus robusta-derived FPMs, eucarobustol E (EE), exerted a strong inhibitory effect against Candida albicans biofilms at a concentration of 16 μg/ml. EE was found to block the yeast-to-hypha transition and reduce the cellular surface hydrophobicity of the biofilm cells. RNA sequencing and real-time reverse transcription-PCR analysis showed that exposure to 16 μg/ml of EE resulted in marked reductions in the levels of expressions of genes involved in hyphal growth (EFG1, CPH1, TEC1, EED1, UME6, and HGC1) and cell surface protein genes (ALS3, HWP1, and SAP5). Interestingly, in response to EE, genes involved in ergosterol biosynthesis were downregulated, while the farnesol-encoding gene (DPP3) was upregulated, and these findings were in agreement with those from the quantification of ergosterol and farnesol. Combined with the obvious elevation of negative regulator genes (TUP1, NRG1), we speculated that EE's inhibition of carbon flow to ergosterol triggered the mechanisms of the negative regulation of hyphal growth and eventually led to biofilm inhibition.

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