scholarly journals ACTIVITY OF CASTOR OIL SEEDS Ricinus communis ETHANOL EXTRACT AGAINST COTTON LEAFWORM Spodoptera littoralis (BOISD.)

2015 ◽  
Vol 6 (12) ◽  
pp. 1711-1725
Author(s):  
H. Dahi ◽  
Walaa Gamil ◽  
Mona Yones
1991 ◽  
Vol 279 (2) ◽  
pp. 495-501 ◽  
Author(s):  
F E Podestá ◽  
W C Plaxton

The kinetic and regulatory properties of cytosolic pyruvate kinase (PKc) isolated from endosperm of germinating castor oil seeds (Ricinus communis L.) have been studied. Optimal efficiency in substrate utilization (in terms of Vmax/Km for phosphoenolpyruvate or ADP) occurred between pH 6.7 and 7.4. Enzyme activity was absolutely dependent on the presence of a bivalent and a univalent metal cation, with Mg2+ and K+ fulfilling this requirement. Mg2+ binding showed positive and negative co-operativity at pH 6.5 (h = 1.6) and pH 7.2 (h = 0.69) respectively. Hyperbolic saturation kinetics were observed with phosphoenolpyruvate (PEP) and K+, whereas ADP acted as a mixed-type inhibitor over 1 mM. Glycerol (10%, v/v) increased the S0.5(ADP) 2.3-fold and altered the pattern of nucleotide binding from hyperbolic (h = 1.0) to sigmoidal (h = 1.79) without modifying PEP saturation kinetics. No activators were identified. ATP, AMP, isocitrate, 2-oxoglutarate, malate, 2-phosphoglycerate, 2,3-bisphosphoglycerate, 3-phosphoglycerate, glycerol 3-phosphate and phosphoglycolate were the most effective inhibitors. These metabolites yielded additive inhibition when tested in pairs. ATP and 3-phosphoglycerate were mixed-type inhibitors with respect to PEP, whereas competitive inhibition was observed for other inhibitors. Inhibition by malate, 2-oxoglutarate, phosphorylated triose sugars or phosphoglycolate was far more pronounced at pH 7.2 than at pH 6.5. Although 32P-labelling studies revealed that extensive phosphorylation in vivo of soluble endosperm proteins occurred between days 3 and 5 of seed germination, no alteration in the 32P-labelling pattern of 5-day-germinated endosperm was observed after 30 min of anaerobiosis. Moreover, no evidence was obtained that PKc was a phosphoprotein in aerobic or anoxic endosperms. It is proposed that endosperm PKc activity of germinating castor seeds is enhanced after anaerobiosis through concerted decreases in ATP levels, cytosolic pH and concentrations of several key inhibitors.


FEBS Letters ◽  
1988 ◽  
Vol 234 (2) ◽  
pp. 275-279 ◽  
Author(s):  
Yan-Ping Yang ◽  
Douglas D. Randall ◽  
Richard N. Trelease

2012 ◽  
Vol 38 ◽  
pp. 64-71 ◽  
Author(s):  
M.J. Perea-Flores ◽  
V. Garibay-Febles ◽  
J.J. Chanona-Pérez ◽  
G. Calderón-Domínguez ◽  
J.V. Méndez-Méndez ◽  
...  

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
El-Sayed Mokbel ◽  
Amal Huesien

Abstract Background The cotton leafworm, Spodoptera littoralis (Boisd.), is a serious economic pest in Egypt. Pest control depends mainly on chemical control with several pesticides include conventional and modern insecticides. Comprehensive analysis of pesticides impacts needs to investigate sublethal effects in addition to lethal effect. Results In the current study, the leaf-dip bioassay method was used to evaluate emamectin benzoate (EMB) sublethal concentrations. Results showed that EMB proved high toxicity against S. littorals with LC50 value of 0.019 mg liter−1. Life table analysis showed that treatments with LC5 and LC15 prolonged larval period, mean longevity of males and females, mean generation time (T), doubling time (DT), adult preovipositional period (APOP), and total preovipositional period (TPOP) compared with control. On the contrary, net reproduction rates (R0), intrinsic rates of increase (r), finite rate (λ), fecundity, gross reproductive rate (GRR), and relative fitness were decreased compared to control. Conclusions The current study clarified that sublethal concentrations of EMB induce adverse effects and suppress the population growth of S. littorals. Our results would be useful to assess the overall effects of EMB on S. littorals and can contribute effectively in pest management.


2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Hend O. Mohamed

Abstract Background The Egyptian cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the major insect pests, causing a significant damage on different cultivated agricultural crops. Developing an alternative non-chemical tool, an effective and environmentally friendly method to suppress pest's infestation is essentially needed. Therefore, biological control by releasing the egg parasitoids could be the most promising tool for integrated pest management. Results This study was designed to evaluate the efficacy of the egg parasitoid, Trichogrammatoidea bactrae Nagaraja (Hymenoptera: Trichogrammatidae) as a bio-control agent against S. littoralis egg masses with different physical characteristics (number of egg layer and degree of scale density) in a no-choice and choice tests, under laboratory conditions. Also, the parasitoids’ fitness in terms of parasitism percentage, developmental period, adults’ emergence percentage, female offspring percentage, and longevity were investigated. The results revealed that T. bactrae wasps had a great ability to parasitize S. littoralis egg masses, but with different rates, related to their layers and scales’ thickness in both tests. The highest parasitism percentage was observed on one-layer eggs, followed by two layers. However, 3-layer eggs were the least preferable one. High numbers of adult emergencies (> 80%) were observed in all tested egg masses, except in the case of 3 layers with high scales. Furthermore, female-biased sex ratios were noticed at all examined eggs, with only the exception of high-scaly eggs with a single layer that recorded the lowest rate (≤ 45%). Besides, the survival of adult female parasitoids was not significantly affected in both tests. Conclusions T. bactrae could be used as a bio-control agent against S. littoralis egg masses with different physical characteristics based on the achieved results.


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