scholarly journals EFFECT OF MATURITY STAGE ON PHYSICAL AND CHEMICAL CHARACTERISTICS AND DETERMINATION OF HARVEST TIME OF SUGAR SNAP PEA PODS

2014 ◽  
Vol 5 (2) ◽  
pp. 305-314 ◽  
Author(s):  
S. El-Seifi ◽  
M. Hassan ◽  
R. El-Bassiouny ◽  
M. Elwan ◽  
I. Nasef
2018 ◽  
Vol 6 (4) ◽  
pp. 351-366
Author(s):  
L. I. Butenko ◽  
S. A. Kuleshova ◽  
J. V. Podgornaya ◽  
L. P. Myikots ◽  
A. B. Dmitriev

Introduction.Nowadays, it is vitally important to develop a method for quantifying melittin not only in apitoxin samples, but also in the products of its processing.The aim of our studyis to investigate physical and chemical characteristics of melittin, the main component of apitoxin, as well as the development of methods for the quantitative determination of melittin in the samples of apitoxin and in the pharmaceutical products derived from apitoxin: “Sophia with apitoxin” cream and “Apizartron” ointment.Materials and methods.The objects of the research were apitoxin and melittin, as well as the samples of “Sophia with apitoxin” cream and “Apizartron” ointment satisfying the requirements of regulatory documentation, produced in lots by domestic and foreign manufacturers. The UV spectra of melittin and apitoxin were registered on SF 103 spectrophotometer in quartz cuvettes with 1 cm thickness. The IR spectra were investigated on the IR-instrument of Fourier – FSM-1201 spectrophotometer, LLC “Infraspek”. The determination of melittin purity was carried out by chromatography.Results and discussion. The basic physical and chemical characteristics were established for melittin as a reference sample and the main component of apitoxin. The melting temperature was: (Tmelt.)=190ºС. In the UV-spectrum there could be watched the absorption maxima corresponding to 2 peaks: λ max = 225±2 nm and 285±2 nm were observed. As an analytical wavelength, it is necessary to choose the peak λ =285 nm, since the peak with λ =225 nm is associated with the absorption of light by the internal (shielded) benzene ring of the tryptophan molecule. The nature of the curve and the position of the maxima of the spectra of melittin and the apitoxin solution coincide, which makes it possible to use melittin as a standard for spectrometric quantitative determination of the active substances in apitoxin and preparations based on apitoxin.Conclusion. The worked out spectrophotometric methods for the quantitative determination of melittin in apitoxin has been validated by the indices of specificity, accuracy, detection limit, quantitative determination limit, linearity.


2021 ◽  
Vol 27 (3) ◽  
pp. 481-495
Author(s):  
Raymundo F. Ramírez-Roacho ◽  
◽  
Maribel Guerrero-Cervantes ◽  
José Á. Prieto-Ruíz ◽  
Melissa Bocanegra-Salazar ◽  
...  

Introduction: In Mexico, there is a lack of information on morphological, physical and chemical characteristics of acorns of Quercus, which makes it difficult to make decisions on the use that can be made to the fruits. Objectives: To identify the morphological, physical and chemical characteristics of Quercus rugosaNée, Q. sideroxyla Humb. & Bonpl. and Q. deserticola Trel. acorns. Materials and methods: From each species, 10 trees were selected and 30 acorns per tree were collected. Mature acorns were characterized morphologically (polar and equatorial diameter, shell weight and total weight) and in physicochemical characterization (moisture, dry matter, ash, crude fiber and protein, ethereal extract and tannins) green and mature acorns were used. Differences among species and among maturity stages were determined with an analysis of variance (P ≤ 0.05) and subsequent Tukey’s test. Results and discussion: Morphology varied among species and among trees of the same species (P < 0.05); Q. deserticola had the largest acorn size (15.69 mm) and weight (1.94 g), and Q. sideroxylaproduced the smallest acorns. Maturity stage and species significantly (P < 0.05) influenced chemical composition; mature acorns had higher content of ethereal extract (8.88 %) and protein (8.40 %). Pearson’s correlation indicated that acorn weight was strongly associated with crown diameter and diameter at breast height. Conclusion: Significant differences were found in morphology and chemical composition of acorns of each species, although they inhabit sites with similar characteristics.


Food Industry ◽  
2018 ◽  
Vol 3 (4) ◽  
Author(s):  
Nataliia V. Nepovinnykh ◽  
Yuri A. Kodatsky ◽  
Oksana N. Klyukina ◽  
Nataliia M. Ptichkin ◽  
Samira Yeganehzad

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