scholarly journals GENETIC DIVERSITY OF RICE BLAST FUNGUS POPULATIONS (Pyricularia grisea) USING MOLECULAR MARKERS

2016 ◽  
Vol 7 (3) ◽  
pp. 57-65 ◽  
Author(s):  
S. El-Wahsh ◽  
Y. El-Refaee ◽  
A. Emeran ◽  
S. Mashaal ◽  
R. Arafa
2020 ◽  
Vol 6 (1) ◽  
pp. 38 ◽  
Author(s):  
Apinya Longya ◽  
Sucheela Talumphai ◽  
Chatchawan Jantasuriyarat

Rice blast disease is caused by the ascomycete fungus Pyricularia oryzae and is one of the most destructive rice diseases in the world. The objectives of this study were investigating various fungal morphological characteristics and performing a phylogenetic analysis. Inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers were used to examine the genetic variation of 59 rice blast fungus strains, including 57 strains collected from different fields in Thailand and two reference strains, 70-15 and Guy11. All isolates used in this study were determined to be P. oryzae by internal transcribed spacer (ITS) sequence confirmation. A total of 14 ISSR primers and 17 pairs of SRAP primers, which produced clear and polymorphic bands, were selected for assessing genetic diversity. A total of 123 polymorphic bands were generated. The similarity index value for the strains ranged from 0.25 to 0.95. The results showed that the blast fungus population in Thailand has both morphological and genetic variations. A high level of genetic variation, or genome adaptation, is one of the fungal mechanisms that could overcome host resistance to avoid host recognition. Results from this research study could bring substantial benefits and ultimately help to understand the blast fungal pathogen genome and the population structure in Thai blast fungus.


2017 ◽  
Vol 61 ◽  
pp. 1-7
Author(s):  
Nguyen Phu Toan ◽  
Pham Thi Thu Ha ◽  
Tran Dang Xuan

Rice blast fungus (Pyricularia grisea) is one of the most problematic pathogen to significantly reduce rice production worldwide. In this study, after being inoculated withP. grisea, changes in phenolic components and antioxidant capacity and correlation with the resistant level against rice blast fungus were investigated. Among screened rice cultivars, AV-3 was the strongest resistant, whereas BII-3 was the most susceptible. It was found that although total contents of phenolics and flavonoids, and antioxidant capacities varied among studied varieties, no significant coefficient with the resistance againstP. griseawas observed. After rice was affected by rice blast fungus, total phenolics and flavonoids were markedly reduced, but in contrast, the DPPH scavenging activities of only the susceptible rice cultivars was reduced. Among the 11 phenolic acids detected, catechol was found only in the tolerant cultivar AV-3, whereas the amount of cinnamic acid was increased after infection. Quantity of vanillin was also promoted, except in the susceptible cultivar BII-3 that was significantly reduced. Findings of this study showed that the resistant level againstP. griseawas proportionally correlated to the antioxidant capacity. Catechol, cinnamic acid, and vanillin may play a role but it needs further elaboration. Observations of this study suggested that the infection of blast disease by reducing amount of phenolics and flavonoids that may weaken the resistance of rice against this detrimental fungus.


Biologia ◽  
2019 ◽  
Vol 74 (9) ◽  
pp. 1181-1189 ◽  
Author(s):  
Athipat Ngernmuen ◽  
Worawit Suktrakul ◽  
Katanyutita Damchuay ◽  
Apinya Longya ◽  
Sureeporn Kate-Ngam ◽  
...  

2000 ◽  
Vol 48 (4) ◽  
pp. 1400-1404 ◽  
Author(s):  
Doris Engelmeier ◽  
Franz Hadacek ◽  
Thomas Pacher ◽  
Srunya Vajrodaya ◽  
Harald Greger

2021 ◽  
Author(s):  
Thanathip Sutthiphai ◽  
Katanyutita Damchuay ◽  
Ram Chandra Neupane ◽  
Apinya Longya ◽  
Tanee Sriwongchai ◽  
...  

2021 ◽  
pp. 103562
Author(s):  
Alice Bisola Eseola ◽  
Lauren S. Ryder ◽  
Míriam Osés-Ruiz ◽  
Kim Findlay ◽  
Xia Yan ◽  
...  

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