scholarly journals Molecular and Genetic Analysis of Leaf Rust Resistance Genes in Two New Egyptian Wheat Cultivars

2017 ◽  
Vol 45 (2) ◽  
pp. 33-52
Author(s):  
Reda Omara ◽  
Khaled Abdelaal
Keyword(s):  
Genetic Analysis ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

2019 ◽  
Vol 14 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Agnieszka Tomkowiak ◽  
Roksana Skowrońska ◽  
Alicja Buda ◽  
Danuta Kurasiak-Popowska ◽  
Jerzy Nawracała ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Field Resistance ◽  
Wheat Cultivars ◽  
Pcr Conditions ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

scholarly journals Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

2013 ◽  
Vol 12 (9) ◽  
pp. 1580-1588 ◽  
Author(s):  
Li-hong SHI ◽  
Na ZHANG ◽  
Ya-ya HU ◽  
Xue-jun WEI ◽  
Wen-xiang YANG ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Chinese Spring Wheat ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Characterization of Leaf Rust Resistance in Hard Red Spring Wheat Cultivars

Plant Disease ◽  
2004 ◽  
Vol 88 (10) ◽  
pp. 1127-1133 ◽  
Author(s):  
L. M. Oelke ◽  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Seedling Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused by Puccinia triticina Eriks., is the most common disease of wheat (Triticum aestivum L.) in the United States and worldwide. The objective of this study was to characterize seedling and adult plant leaf rust resistance in hard red spring wheat cultivars grown in Minnesota, North Dakota, and South Dakota, and postulate the identity of the seedling leaf rust resistance genes in the cultivars. Twenty-six cultivars, near-isogenic lines of Thatcher wheat that differ for single leaf rust resistance genes, and three wheat cultivars with known leaf rust resistance genes, were tested with 11 different isolates of leaf rust collected from the United States and Canada. The leaf rust infection types produced on seedling plants of the cultivars in greenhouse tests were compared with the infection types produced by the same isolates on the Thatcher near-isogenic lines to postulate which seedling leaf rust resistance genes were present. Seedling leaf rust resistance genes Lr1, Lr2a, Lr10, Lr16, Lr21, and Lr24 were postulated to be present in spring wheat cultivars. Seedling genes Lr3, Lr14a, and Lr23 likely were present in some cultivars but could not be clearly identified in this study. Most of the cultivars had some level of adult plant leaf rust resistance, most likely due to Lr34. Cultivars that had seedling resistance genes Lr1, Lr2a, Lr10, or Lr16 had poor to intermediate levels of leaf rust resistance in field plots. Cultivars with combinations of seedling resistance genes Lr16 and Lr24 with additional adult plant resistance were highly resistant to leaf rust.

scholarly journals Seedling and Slow Rusting Resistance to Leaf Rust in Chinese Wheat Cultivars

Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Z. F. Li ◽  
X. C. Xia ◽  
Z. H. He ◽  
X. Li ◽  
L. J. Zhang ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Slow Rusting ◽  
Cropping Seasons ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Identification of resistance genes is important for developing leaf rust resistant wheat (Triticum aestivum) cultivars. A total of 102 Chinese winter wheat cultivars and advanced lines were inoculated with 24 pathotypes of Puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. These genotypes were also planted in the field for characterization of slow rusting responses to leaf rust in the 2006–07 and 2007–08 cropping seasons. Fourteen leaf rust resistance genes—Lr1, Lr2a, Lr3bg, Lr3ka, Lr14a, Lr16, Lr17a, Lr18, Lr20, Lr23, Lr24, Lr26, Lr34, and LrZH84—either singly or in combinations, were postulated in 65 genotypes, whereas known resistance genes were not identified in the other 37 accessions. Resistance gene Lr26 was present in 44 accessions. Genes Lr14a and Lr34 were each detected in seven entries. Lr1 and Lr3ka were each found in six cultivars, and five lines possessed Lr16. Lr17a and Lr18 were each identified in four lines. Three cultivars were postulated to possess Lr3bg. Genes Lr20, Lr24, and LrZH84 were each present in two cultivars. Each of the genes Lr2a and Lr23 may exist in one line. Fourteen genotypes showed slow leaf rusting resistance in two cropping seasons.

scholarly journals Physiologic Specialization of Puccinia triticina in Canada in 1998

Plant Disease ◽  
2001 ◽  
Vol 85 (2) ◽  
pp. 155-158 ◽  
Author(s):  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Bread Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

In 1998, leaf rust of wheat (Triticum aestivum), caused by Puccinia triticina, was widespread throughout the prairies of western Canada. Warm summer temperatures with frequent dew periods favored spread of the disease in wheat fields in Manitoba and Saskatchewan. The Canada Prairie Spring wheat cultivars (AC Vista, AC Foremost, AC Crystal) were susceptible to leaf rust, while the bread wheat cultivars with leaf rust resistance genes Lr16 and Lr13 or Lr34 (AC Majestic, AC Domain, AC Barrie) had high to moderate levels of leaf rust infections. Bread wheat cultivars AC Cora, AC Minto, Pasqua, and McKenzie had trace to low levels of leaf rust infection. Thirty-four virulence phenotypes of P. triticina were identified on 16 Thatcher lines, which are near-isogenic for leaf rust resistance genes. Phenotypes with virulence to Lr16 increased to 25% of isolates in Manitoba and Saskatchewan in 1998. Forty-three isolates were also tested for virulence to plants with the adult plant resistance genes Lr12, Lr13, Lr34, and Lr13,34. Most isolates had virulence to Lr12 and Lr13. All isolates had lower infection type on adult plants with Lr34 compared with Thatcher.

scholarly journals Molecular Markers Identification of Leaf Rust Resistant Genes Lr19, Lr21, Lr24, Lr47 and Lr51 in Selected Egyptian Wheat Cultivars

2015 ◽  
Vol 4 (2) ◽  
pp. 55-62
Author(s):  
Ashraf M.M. Abdelbacki ◽  
Reda I. Omara ◽  
Nor E.K. Soliman ◽  
Mohammed A. Najeeb
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Dynamic Change ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Rust Disease ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused by Puccinia triticina is a common and widespread disease of bread wheat (Triticum aestivum L.), in Egypt. Host resistance is the most economical, effective and ecologically sustainable method for controlling the disease. Molecular markers help to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. The objective of this study was to evaluate and detect leaf rust resistance genes in Egyptian wheat cultivars. Ten out of fifteen cultivars were resistance to leaf rust disease in four locations i.e., Dakahlia, Kafr el-Sheikh, Beheira and Sharqia during seasons 2011/2012 and 2012/2013. As for, using specific SSR primers proved that Lr19 was present in five cultivars i.e., Sakha-95, Gemmeiza-9, Gemmeiza-10, Misr-1 and Misr-2. Lr21. Lr24, Lr47, and Lr51 were detected in all tested cultivars. These genes should be taken into consideration in wheat breeding programs for successful rust resistance. Furthermore these materials can be used as a parent for plant breeders to add new effective resistance genes to their breeding materials because of the dynamic change of leaf rust races which can breakdown the resistance.

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