scholarly journals Effect of Gibberellic Acid, Indole Acetic Acid and Ethylene Gas on The In vitro Biocontrol Activity of Trichoderma harzianumRefai Against Alternaria solani and Botrytis cinerea

2008 ◽  
Vol 43 (1) ◽  
pp. 53-74
Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.


1974 ◽  
Vol 29 (1-2) ◽  
pp. 39-41 ◽  
Author(s):  
H. Veen

Abstract Studies in vitro on the binding of phospholipids with IAA, α-NAA and some of their biologically inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth.


1970 ◽  
Vol 33 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Sridhar Gutam ◽  
Virendra Nath ◽  
GC Srivastava

A pot experiment was conducted in the rabi (post rainy) seasons of 2001 and 2002 to study the genotypic differences in grain growth rate and endogenous hormonal content in the developing grains of hexaploid and tetraploid wheat. The endogenous hormonal contents of grains in both the ploidy levels had changed in sequence. At 5 days after anthesis (DAA), gibberellic acid (GA3); at 15 DAA (rapid growth phase), indole-acetic acid (IAA); at 25 DAA (dough stage), abscisic acid (ABA) were maximum. At 35 DAA, all the endogenous hormonal level decreased and among the hormones, ABA was highest followed by IAA and GA3. Hexaploids recorded higher concentrations of endogenous hormones (13.38% IAA, 17.89% GA3, and 14.7% ABA) on fresh weight basis and resulted in higher seed weight (56.99 mg/grain) and grain growth rate (0.009 g/g/day) compared to tetraploids (49.08 mg/grain; 0.008 g/g/day) on dry weight basis by better mobilization of photosynthates during grain filling. Key Words: Grain growth rate, hormones, indole-acetic acid, gibberellic acid, abscisic acid. doi:10.3329/bjar.v33i3.1608 Bangladesh J. Agril. Res. 33(3) : 493-502, September 2008


2010 ◽  
Vol 67 (6) ◽  
pp. 743-746 ◽  
Author(s):  
Rafael Leandro Figueiredo de Vasconcellos ◽  
Mylenne Calciolari Pinheiro da Silva ◽  
Carlos Marcelo Ribeiro ◽  
Elke Jurandy Bran Nogueira Cardoso

Actinobacteria are capable of playing several different roles in soil ecosystems. These microorganisms affect other organisms by producing secondary metabolites and are responsible for the degradation of different complex and relatively recalcitrant organic compounds. In our survey of actinobacteria isolated from the rhizosphere of Araucaria angustifolia, five culture media (AI, WYE, YCED, MSSC and LNMS) were compared for their effectiveness in isolating these microorganisms. When summing up all the isolates randomly obtained, we got 103 isolates. After isolation, the phosphate-solubilizing ability and the "in vitro" production of indole-acetic acid and chitinases were evaluated. The AI medium was ineffective for actinobacteria isolation, when it was compared with the other four culture media. Indole-acetic acid and chitinase were produced by respectively 36% and 24% of the strains tested. However, only 2% of the 103 strains presented some phosphate-solubilizing ability. These results demonstrate the biotechnological potential of these microorganisms.


2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Ummi Lathyfah ◽  
Endah Rita Sulistya Dewi

Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi IndoleAcetid Acid (IAA) terhadap pertumbuhan tunas pisang Barangan (Musaacuminate L. triploid AAA.) serta konsentrasi yang optimum dalam kultur invitro. Penelitian ini dilakukan di Kebun Benih Holtikultura (KBH) KecamatanSalaman, Magelang. Penelitian dilaksanakan selama enam minggu, dimulaiMaret-April 2014. Penelitian menggunakan Rancangan Acak Lengkap (RAL)dengan 4 perlakuan dan 4 kali ulangan yaitu perlakuan 1 (BP1) menggunakan0 ppm IAA, pelakuan 2 (BP2) menggunakan 0,1 ppm IAA, perlakuan 3 (BP3)menggunakan 0,3 ppm IAA, dan perlakuan 4 (BP4) menggunakan 0,6 ppm.Data yang diperoleh dianalisis dengan menggunakan Uji Jarak Ganda Duncan(UJGD). Hasil Analisis Varians menunjukkan konsentrasi paling optimal untukmeningkatkan jumlah anakan tunas 0,3 ppm IAA dan 0,6 ppm untukmeningkatkan jumlah akar, panjang daun, serta panjang batang semu.Kesimpulan dari penelitian ini yaitu variasi konsentrasi Indole Acetic Acid(IAA) secara umum tidak berpengaruh nyata terhadap pertumbuhan pisang Barangan (Musa acuminata L. triploid AAA), meskipun terdapat satuparameter yang berpengaruh nyata, yaitu jumlah anakan tunas pisangBarangan.Kata kunci : Indole Acetic acid (IAA), kultur in vitro, pisang Barangan.


2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .


1984 ◽  
Vol 179 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
K.K. Chattopadhyay ◽  
R.N. Bhattacharyya ◽  
P.S. Basu

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