scholarly journals EFFECT OF PHYTOHORMONES PRODUCED BY PGPR (RHIZOBIUM) STRAINS ON IN-VITRO PROPAGATION OF DATE PALM (Phoenix dactylifera L.) ZAGHLOUL CULTIVAR

2009 ◽  
Vol 87 (1) ◽  
pp. 267-276
Author(s):  
SHEREF EL-SHARABASY ◽  
ATEF A. RAGAB
Keyword(s):  
In Vitro Propagation ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L ◽  
Rhizobium Strains

scholarly journals Propagasi in vitro tanaman kurma (Phoenix dactylifera L.) pada bioreaktor dengan perendaman sesaat

2020 ◽  
Vol 88 (2) ◽  
Author(s):  
Rizka Tamania SAPTARI ◽  
Masna Maya SINTA ◽  
Imron RIYADI ◽  
. PRIYONO ◽  
. SUMARYONO
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
In Vitro Propagation ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Embryogenic Calli ◽  
Temporary Immersion Bioreactor ◽  
Phoenix Dactylifera L ◽  
Immersion Period

The cultivation of date palm in Indonesia has increased since the last decade. However, the superior date palm seedlings are still limited and most of them are imported from other countries. The mass supply of superior date palm seedlings can be provided by in vitro propagation in the bioreactor. Therefore, the research was conducted to develop a protocol of date palm in vitro propagation by using Temporary Immersion Bioreactor (TIB). The in vitro propagation was carried out through somatic embryogenesis technique using meristematic tissues isolated from offshoots of date palm female clone cv. Zambli as explants. The explants were sterilized and then cultured to produce embryogenic calli and somatic embryos. Afterwards, somatic embryos germination and plantlets formation were conducted in TIB with treatments of immersion period: 3, 10, and 30 minutes every 6 hours, with 8 replications, The results showed that the optimal somatic embryo germination in TIB was with the immersion period of 30 min every 6 h, resulting in the most formation of shoots and fresh biomass weight increment up to nearly threefold in 6 weeks. Thereafter, plantlets formation in TIB with immersion period of 10 min and 30 min every 6 h exhibited similar performances in producing more plantlets with higher total fresh weight and better vigor than those of 3 min every 6 h. However, there were more rooted plantlets in the TIB with immersion period of 10 min every 6 h. Based on the results, an in vitro propagation protocol via somatic embryogenesis in TIB has been successfully developed for mass propagation of date palm cv. Zambli, which produced plantlets with good vigor and rooting.

scholarly journals 2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

2021 ◽  
Author(s):  
Emna Baklouti ◽  
Thierry Beulé ◽  
Amèni Nasri ◽  
Amal Ben Romdhane ◽  
Riadh Drira ◽  
...  
Keyword(s):  
Date Palm ◽  
Specific Activity ◽  
Phoenix Dactylifera ◽  
Proline Content ◽  
Epigenetic Changes ◽  
Negative Effects ◽  
Somaclonal Variations ◽  
Phoenix Dactylifera L ◽  
Free Media

Abstract The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L− 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g− 1 to 2973 nmol g− 1 FW when 40 mg L− 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA); likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8–18.93%.

scholarly journals In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)

2014 ◽  
Vol 23 (2) ◽  
pp. 211-219
Author(s):  
Md. Abul Kalam Azad ◽  
Hasnatul Arefin ◽  
Md. Amzad Hossain
Keyword(s):  
Date Palm ◽  
Callus Formation ◽  
Shoot Proliferation ◽  
Phoenix Dactylifera ◽  
Shoot Induction ◽  
In Vitro Morphogenesis ◽  
Shoot Initiation ◽  
Phoenix Dactylifera L ◽  
Young Leaves

After inoculation of young leaves of date palm offshoot required about six months to complete the morphogenesis process. Fourteen weeks were required for embryogenic callus formation under continuous dark condition and nine weeks for shoot initiation (under 16/8 h light/dark). The highest number of explants (80%) produced callus in modified MS containing 5 mg/l 2,4-D + 2 mg/l 2ip. Sixty per cent of explants produced callus in the modified medium containing 5 mg/l 2,4-D + 5 mg/l NAA. while only 50 per cent of the explants formed callus in the same medium when supplemented with only 5 mg/l 2,4-D. The induced calli were transferred to modified MS for shoot proliferation. A combination of two cytokines showed better performance than single ones in shoot induction. The highest percentage (70) of shoot developed in modified MS containing 2 mg/l BAP + 1 mg/l Kn. Forty per cent shoot induction was found in the same medium supplemented with 2 mg/l of BAP. Thirty per cent shoot formed in MS containing 1 mg/l of Kn. The shoots were subcultured at three- four week intervals throughout culture duration. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17522 Plant Tissue Cult. & Biotech. 23(2): 211-219, 2013  (December)

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