Physical properties of different Pulp Capping Materials and Histological Analysis of their effect on Dogs’Dental Pulp Tissue Healing

2018 ◽  
Vol 64 (3) ◽  
pp. 2657-2667
Author(s):  
Mai Hamdy ◽  
Dalia Fayyad ◽  
Mohamed Eldaharawy ◽  
Enas Hegazy
Keyword(s):  
Physical Properties ◽  
Histological Analysis ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Tissue Healing

scholarly journals Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Alicja Nowicka ◽  
Ryta Łagocka ◽  
Mariusz Lipski ◽  
Mirosław Parafiniuk ◽  
Katarzyna Grocholewicz ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Third Molar ◽  
Adhesive System ◽  
Histological Evaluation ◽  
Control Group ◽  
Single Bond ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Hydroxide Group ◽  
Dentin Adhesive

Objective. This study presents a clinical and histological evaluation of human pulp tissue responses after direct capping using a new dentin adhesive system. Methods. Twenty-eight caries-free third molar teeth scheduled for extraction were evaluated. The pulps of 22 teeth were mechanically exposed and randomly assigned to 1 of 2 groups: Single Bond Universal or calcium hydroxide. Another group of 6 teeth acted as the intact control group. The periapical response was assayed, and a clinical examination was performed. The teeth were extracted after 6 weeks, and a histological analysis was performed. The pulp status was assessed, and the thickness of the dentin bridge was measured and categorized using a histological scoring system. Results. The clinical phase was asymptomatic for Single Bond Universal patients. Patients in the calcium hydroxide group reported mild symptoms of pain, although the histological examination revealed that dentin bridges with or without limited pulpitis had begun forming in each tooth. The universal adhesive system exhibited nonsignificantly increased histological signs of pulpitis (P>0.05) and a significantly weaker thin mineralized tissue layer (P<0.001) compared with the calcium hydroxide group. Conclusion. The results suggest that Single Bond Universal is inappropriate for human pulp capping; however, further long-term studies are needed to determine the biocompatibility of this agent.

scholarly journals Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite

2016 ◽  
Vol 63 (4) ◽  
pp. 183-192 ◽  
Author(s):  
Marijana Popović Bajić ◽  
Violeta Petrović ◽  
Vanja Opačić Galić ◽  
Vesna Danilović ◽  
Vukoman Jokanović ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Healing Process ◽  
Glass Ionomer Cement ◽  
Control Group ◽  
Therapeutic Effects ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Nanostructural Material ◽  
Experimental Group

Summary Introduction Direct pulp capping is an important therapeutic method that has goal to provide formation of dentin bridge and healing process of the pulp. The aim of this study was to investigate the effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite on exposed dental pulp in Vietnamese pigs. Material and Methods The study was conducted on 30 teeth of two Vietnamese pigs (Sus scrofa verus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared with a small round bur and pulp horn was exposed. In the first experimental group (10 teeth) the perforation was covered with new nanostructural material based on calcium silicate systems (CS). In the second experimental group, the perforation was covered with compound of calcium silicate systems and hydroxyapatite (HA-CS) (10 teeth). In the control group, exposed pulp was covered with Pro Root MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA) (10 teeth). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in all groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion Histological analysis indicated favorable therapeutic effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite for direct pulp capping in teeth of Vietnamese pigs.

scholarly journals THE EFFECT OF CALCIUM HYDROXIDE-PROPOLIS ON THE NUMBER OF MACROFAG CELLS ON THE WISTAR RAT DENTAL WITH PULPA PERFORMANCE

2020 ◽  
Vol 10 (2) ◽  
pp. 58
Author(s):  
Setyabudi Setyabudi ◽  
Devi Eka Juniarti ◽  
Ira Widjiastuti ◽  
Maughfirah Shintya Fathori
Keyword(s):  
Calcium Hydroxide ◽  
Cellular Response ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Wistar Rat ◽  
Control Group ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Macrophage Cells ◽  
Combined Application

Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwithcention. Macrophage cell is calculated histopathologically by using compound light microscope on the 3rd day with 400x magnification. Results: The Kruskal-Wallis test results showed significant differences in macrophages after the combination of calcium hydroxide and propolis (p = 0,000). The combined application of calcium hydroxide and propolis showed higher macrophages than the application of calcium hydroxide and the control group. Conclusion: Application of calcium hydroxide - propolis combination was proven increase the number of macrophage cells in Wistar Rats (Rattus Norvegicus) with pulp perforation

scholarly journals Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

2019 ◽  
Vol 12 (4) ◽  
pp. 182-186
Author(s):  
Mozammal Hossain ◽  
Mahmood Sajedeen ◽  
Yukio Nakamura
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Normal Function ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Exposure Site ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

Visible Light-Induced Crosslinkable Gelatin for Direct Pulp Capping

2012 ◽  
Vol 529-530 ◽  
pp. 543-546
Author(s):  
Mariko Nakamura ◽  
Yasuhiro Yoshida ◽  
Yoshihiro Ito
Keyword(s):  
Soft Tissue ◽  
Visible Light ◽  
Dental Pulp ◽  
Dental Materials ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Dental Pulp Tissue

Direct pulp capping involves the application of dental materials to the exposed pulp in an attempt to act as a barrier, protect the dental pulp complex and preserve its vitality. The materials for direct pulp capping should ideally adhere to dental pulp tissue as soft tissue and dentin as hard tissue with tight sealing. We therefore developed visible light-induced crosslinkable gelatin which is capable of adhesive to soft tissue and hard tissue.

scholarly journals Comparative study of fibrin and chemical synthetic sealant on dural regeneration and brain damage

2013 ◽  
Vol 19 (6) ◽  
pp. 736-743 ◽  
Author(s):  
Kiyoshi Ito ◽  
Tetsuyoshi Horiuchi ◽  
Kiyomitsu Oyanagi ◽  
Tetsuo Nomiyama ◽  
Kazuhiro Hongo
Keyword(s):  
Brain Damage ◽  
Fibrin Glue ◽  
Histological Analysis ◽  
Healing Process ◽  
Abscess Formation ◽  
Cerebrospinal Fluid Leakage ◽  
Parietal Lobes ◽  
Tissue Healing ◽  
Dural Defects

Object Several materials, such as polyethylene glycol (PEG) hydrogel and fibrin glue, have been used to seal dural incisions after brain and spinal surgeries. Although the use of PEG sealant is gaining popularity, it can be associated with postoperative cerebrospinal fluid leakage and infection. However, the reasons for this association are currently unknown. The present study aimed to investigate the effects of PEG sealant and fibrin glue on wound healing and brain damage in vivo. Methods Oval-shaped bone defects and dural defects were created bilaterally over the parietal lobes of 22 Japanese white rabbits. The dural defects were covered with 0.5 ml of fibrin glue on one side and 0.5 ml of PEG sealant on the other side. Dural regeneration and brain damage were investigated in each harvested brain and dura mater using light microscopy. Results Dural regeneration was more effective in the presence of fibrin glue than it was with PEG sealant (p = 0.014). Of the 22 rabbits, 11 showed thick (Grades ++ and +++) dural regeneration by 28 days postsurgery in the hemisphere where fibrin glue was used, whereas Grade +++ dural regeneration was not observed in the PEG hydrogel hemisphere, and only 4 rabbits showed Grade ++ regeneration. Abscess and granulation formation also tended to be more severe when PEG hydrogel sealant was used. No Grade ++ granulation/abscess formation was observed with fibrin glue, and Grade + was only observed in 13 of 22 rabbits. Conversely, with PEG hydrogel sealant, only 2 rabbits did not show granulation/abscess formation, and Grade +, ++, and +++ granulation/abscess formation was observed in 8, 7, and 5 rabbits, respectively. The extent of cortical damage was significantly greater in rabbits with abscesses and granulations, compared with rabbits without these lesions (p = 0.007). Conclusions Dural regeneration tended to occur more rapidly with fibrin glue, whereas granulation was more likely with PEG hydrogel sealant, which led to postoperative complications. Histological analysis indicated that PEG hydrogel sealant inhibited the normal tissue healing process and that outcomes were improved by the use of fibrin glue.

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