scholarly journals ESEM ANALYSIS OF ENAMEL SURFACE MORPHOLOGY ETCHED WITH ER,CR:YSGG LASER AND PHOSPHORIC ACID: IN VITRO STUDY

2017 ◽  
Vol 63 (1) ◽  
pp. 941-947
Author(s):  
Sahar El Halim ◽  
Rasha Raafat ◽  
Amr ElGanzory
Keyword(s):  
Surface Morphology ◽  
Phosphoric Acid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

scholarly journals Bonding of Resin Composite Luting Cements to Zirconium Oxide by Two Air-particle Abrasion Methods

10.2341/05-22 ◽  
2006 ◽  
Vol 31 (2) ◽  
pp. 248-255 ◽  
Author(s):  
O. Kumbuloglu ◽  
L. V. J. Lassila ◽  
A. User ◽  
P. K. Vallittu
Keyword(s):  
Phosphoric Acid ◽  
Clinical Relevance ◽  
Zirconium Oxide ◽  
Resin Composite ◽  
In Vitro Study ◽  
Vitro Study ◽  
Luting Cements ◽  
Relyx Unicem

Clinical Relevance The results of this in vitro study suggest that, in combination with air-particle abrasion methods, Panavia F and RelyX Unicem resin composite luting cements with phosphoric-acid methacrylate content provide a strong resin bond to zirconium oxide.

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