The Evaluation of Antimicrobial and Antibiofilm Activity of Bioactive Compounds Obtained from Aspergillus Sclerotiorum

The in Vitro Antibiofilm Activity of Waterfall and Marine Bacteria Against Human Bacterial Pathogens

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v13i2.14926 ◽

2020 ◽

Vol 13 (2) ◽

pp. 250-262

Author(s):

Stella Magdalena ◽

Natassa Rustandi ◽

Yogiara Yogiara

Keyword(s):

Quorum Sensing ◽

16S Rrna ◽

Bioactive Compounds ◽

Marine Bacteria ◽

Bacterial Pathogens ◽

Quorum Quenching ◽

Health Concern ◽

Effective Control ◽

Antibiofilm Activity ◽

Antibiofilm Agents

AbstrakKeterlibatan biofilm pada infeksi kronis dan pada permukaan peralatan medis selalu menjadi wacana penting bagi kesehatan umum di dunia. Biofilm bakteri berkaitan dengan tingkat resistensi terhadap antibiotik yang menjadikan infeksi sulit untuk diobati. Untuk mengatasi masalah ini, pengendalian yang efektif perlu diimplementasikan, seperti penerapan senyawa antibiofilm. Beberapa tahun terakhir, lingkungan akuatik menjadi salah satu sumber potensi penghasil senyawa bioaktif, termasuk senyawa antibiofilm. Tujuan dari penelitian ini yaitu menapis dan mengkarakterisasi bakteri asal air terjun dan laut yang diperoleh dari beberapa lokasi di Indonesia, sebagai penghasil aktivitas antibiofilm. Isolat dievaluasi berdasarkan kemampuan aktivitas antimikroba terhadap enam bakteri patogen dan diikuti dengan penapisan senyawa antibiofilm. Sebanyak 11 dari 65 isolat menunjukkan aktivitas quorum sensing atau quorum quenching, dan hanya terdapat satu isolat yang memiliki aktivitas keduanya. Supernatan kesebelas isolat menunjukkan penghambatan pembentukan biofilm setidaknya terhadap satu patogen dengan metode uji biofilm statis. Karakterisasi senyawa bioaktif dari lima isolat yang terpilih menunjukkan aktivitas senyawa yang berbeda, seperti karbohidrat, protein, dan asam nukleat. Sekuensing gen penyandi 16S rRNA menetapkan kelima isolat tersebut berada dalam dua genus yang berbeda, Vibrio (WK2.4, WK2.6, and WK2.3) dan Pseudomonas (S1.2 dan S1.3). Penelitian ini memberikan wawasan baru terhadap pencarian kandidat bakteri akuatik sebagai agen antibiofilm yang potensial. Abstract Biofilm involvement in chronic infections and on the surface of medical equipments have been considered as public health concern worldwide. Bacterial biofilm is related to antibiotic resistance that made the diseases difficult to treat. An effective control strategy should be implemented, for example, by applying antibiofilm agents. Recently, concerns has been given to aquatic environment as potential sources of bioactive compounds, including the antibiofilm compounds. This study aimed to screen and characterize waterfall and marine bacteria obtained from several locations in Indonesia which have antibiofilm activity. The isolates were first evaluated for their antimicrobial activity against six bacterial pathogens and followed by antibiofilm screening. Eleven out of 65 isolates showed quorum sensing or quorum quenching activity, and one of them showed both activities. Supernatants of 11 isolates inhibited biofilm formation of at least one pathogen by using static biofilm assay. Bioactive compounds characterization of the selected five isolates revealed the presence of different compounds, such as carbohydrates, proteins, and nucleic acids. The 16S rRNA gene sequencing analysis classified five isolates into two different genera, Vibrio (WK2.4, WK2.6, and WK2.3) and Pseudomonas (S1.2 and S1.3). The present study provides insights into the discovery of aquatic bacteria candidates as antibiofilm agents.

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Phytocompounds of Curcuma longa extract are more effective against bacterial biofilm than pure curcumin only: An in-vitro and in-silico analysis

Kuwait Journal of Science ◽

10.48129/kjs.v48i2.8310 ◽

2021 ◽

Vol 48 (2) ◽

Author(s):

Dibyajit Lahiri ◽

◽

Moupriya Nag ◽

Soumik Dey ◽

Bandita Dutta ◽

...

Keyword(s):

Bioactive Compounds ◽

In Silico ◽

Methyl Palmitate ◽

Curcuma Longa ◽

Research Work ◽

Bioactive Compound ◽

Bacterial Biofilm ◽

Antibiofilm Activity ◽

Electron Microscopic ◽

In Silico Studies

Bioactive compounds are the group of secondary metabolites of plants that have a potent impact on antimicrobial and antibiofilm agents. Although Curcuma longa (turmeric) is well known for its antimicrobial activity, the question arises if curcumin, the primary bioactive compound is only responsible for it or the synergistic and simultaneous contribution of more than one bioactive compound are responsible for this antibiofilm efficacy. The research work aims to determine the efficacy of the extract Curcuma longa has a higher potential of antimicrobial and antibiofilm activity than the purchased curcumin and standard antibiotic. Present work was initiated with GC-MS analysis of the ethanolic extract of Curcuma longa (turmeric) and showed that in addition to curcumin, methyl palmitate de-hydro zingerone had a higher percent of availability within the extract. The in-silico studies also showed that when targeted upon Gram-positive biofilm-forming protein of Staphylococcus aureus (3TIP), curcumin alone had a binding constant value of -6.33 Kcal/mol but showed a value of -17.811 Kcal/mol when acted in association with Dehydrozingerone. Similarly, the binding constant's value changed from -6.07 Kcal/mol to - 23.844 Kcal/mol, when Gram-negative biofilm-forming protein (3ZYB) of Pseudomonas aeruginosa was acted upon by curcumin only and in association with methyl palmitate, respectively. Lower minimum inhibitory concentration (MIC) and higher effectivity in reducing the bacterial quorum sensing (QS) activity of the turmeric extract than pure Curcumin indicated the higher antimicrobial and antibiofilm efficiency of the extract, respectively. This indicated clearly that the synergistic action of all the bioactive compounds imparts the antibiofilm activity of turmeric. The result was further confirmed by the scanning electron microscopic (SEM) studies, fluorescent microscopic studies, and FTIR analysis of EPS as well.

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Antioxidative Potential, Antibiofilm Activity and Partial Chemical Characterization of Cultured Broth after Pleurotus ferulae Mycelium Cultivation

Revista de Chimie ◽

10.37358/rc.18.11.6669 ◽

2018 ◽

Vol 69 (11) ◽

pp. 2996-3000

Author(s):

Emanuel Vamanu ◽

Oana Livadariu ◽

Ionela Sarbu ◽

Mihaela Ene ◽

Ioan Dopcea

Keyword(s):

Bioactive Compounds ◽

Ph Value ◽

Carbon Sources ◽

Chemical Characterization ◽

Antibiofilm Activity ◽

Optimum Level ◽

Bacterial Strains ◽

Culture Environment ◽

Fermentation Parameters

The purpose of the present study was to assess the antioxidant effect, antibiofilm activity, and partial characterization of the main groups of bioactive compounds of mushrooms, under the influence of the composition of the relevant culture environment and different fermentation parameters of the Pleurotus (P.) ferulae mushroom mycelium. One used five carbon sources (1.0 g, 2.5 g, and 5.0 g/100 mL), which included glucose, sorbitol, starch, lactose, and sucrose, and of the fermentation parameters, the variation was characteristic for temperature (21, 23, and 25�C), and the pH values varied between 5 and 8. Following a review of the data and the statistical correlation of results, an optimum level of culture environment was found: 2.5 g/100 mL of glucose, at a temperature of 21�C, and a pH value of 5. Under such circumstances, the antioxidant activity exhibited a certain correlation with the level of the primary bioactive compounds (phenolic acids, for instance), the antibiofilm effect against four bacterial strains, and the outcomes were confirmed by showing the essential statistical indices.

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Quantification of anti-quorum sensing and antibiofilm activity of Phyllosphere bacteria against food spoilage bacteria

Nova Biotechnologica et Chimica ◽

10.36547/nbc.919 ◽

2021 ◽

pp. e919

Author(s):

Valencia Vanessa ◽

Diana Elizabeth Waturangi

Keyword(s):

Quorum Sensing ◽

Bioactive Compounds ◽

Large Population ◽

Cell Communication ◽

Antibiofilm Activity ◽

Food Spoilage ◽

Biofilm Inhibition ◽

Sensing Mechanism ◽

Spoilage Bacteria ◽

Phyllosphere Bacteria

Food spoilage and microbial contamination require attention during the food production process since the presence of these bacteria can create problems including the formation of biofilms produced by these bacteria. Biofilm formations are initiated through cell-to-cell communication which is called quorum sensing mechanism. Hence, inhibition of this communication mechanism could be one of the solutions to inhibit biofilm formation. Therefore, exploration of bioactive compounds from various sources including hyllosphere bacteria with anti-quorum sensing inhibition activities is important. Phyllosphere bacteria are a community of bacteria found on the surface of plant leaves at a very large population. These bacteria can produce bioactive compounds that can inhibit quorum sensing mechanism. In this study, 54 phyllosphere bacteria isolates were tested, 8 bacterial isolates had potential effect to inhibit quorum sensing. From biofilm inhibition assay, the highest percentages were showed by ifferent phyllosphere isolates against each pathogen. Whereas, for biofilm destruction assay, JB 8F isolate had the highest percentage of destruction biofilm activity against biofilm formed by Bacillus cereus and Shewanella putrefaciens. Eight isolates of phyllosphere bacteria had the potential as quorum quencher and anti-biofilm agents, both for inhibition and destruction of biofilm.

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Antimicrobial, Antibiofilm, and Antioxidant Activity of Functional Poly(Butylene Succinate) Films Modified with Curcumin and Carvacrol

Materials ◽

10.3390/ma14247882 ◽

2021 ◽

Vol 14 (24) ◽

pp. 7882

Author(s):

Łukasz Łopusiewicz ◽

Szymon Macieja ◽

Artur Bartkowiak ◽

Mirosława El Fray

Keyword(s):

Bioactive Compounds ◽

Food Packaging ◽

Antibiofilm Activity ◽

Biodegradable Films ◽

Butylene Succinate ◽

E Coli ◽

Industry Waste ◽

Potential Applications ◽

Bifunctional Properties

The use of food industry waste as bioactive compounds in the modification of biodegradable films as food packaging remains a major challenge. This study describes the preparation and bioactivity characterization of poly(butylene succinate) (PBS)-based films with the addition of the bioactive compounds curcumin (CUR) and carvacrol (CAR). Films based on PBS modified with curcumin and carvacrol at different concentration variations (0%/0.1%/1%) were prepared by solvent casting method. The antioxidant, antimicrobial, and antibiofilm properties were investigated against bacteria (Escherichia coli, Staphylococcus aureus) and fungi (Candida albicans). As a result of the modification, the films exhibited free radicals scavenging (DPPH up to 91.47% and ABTS up to 99.21%), as well as antimicrobial (6 log, 4 log, and 2 log reductions for E. coli, S. aureus, and C. albicans, respectively, for samples modified with 1% CUR and 1% CAR) activity. Moreover, antibiofilm activity of modified materials was observed (8.22–87.91% reduction of biofilm, depending on bioactive compounds concentration). PBS films modified with curcumin and carvacrol with observed bifunctional properties have many potential applications as active packaging.

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Screening of antibiofilm and anti-quorum sensing activty of Actinomycetes isolates extracts against aquaculture pathogenic bacteria

BMC Microbiology ◽

10.1186/s12866-020-02022-z ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Gloria Raissa ◽

Diana Elizabeth Waturangi ◽

Dinamella Wahjuningrum

Keyword(s):

Quorum Sensing ◽

Bioactive Compounds ◽

Inhibitory Activity ◽

Pathogenic Bacteria ◽

Bioactive Compound ◽

Artemia Salina ◽

Antibiofilm Activity ◽

Biofilm Structure ◽

Antibiofilm Agents ◽

The Impact

Abstract Background Indonesia is the third largest producer of fish and other aquaculture products in the world, making this industry a major contributor in the economy of Indonesia. However, this industry continually overcome challenges, one of them are bacterial outbreaks. In addition, the emergence of these bacterial outbreaks were worsen due to the biofilm produced by many significant pathogenic bacteria and the impact of increased antibiotic resistance. These issues have become a global concern, because antibiotics are currently one of the main treatments available to overcome this problems. Therefore, studies aimed at finding and characterizing bioactive compounds to combat these issues. In this study actinomycetes isolates were screened and characterized for their bioactive compounds produced which have inhibitory and destructive activity and also QS inhibitors against biofilm structure of aquatic pathogenic bacteria, such as Vibrio harveyi, A. hydrophila, and S. agalactiae. Result Extracts (20 mg/mL) produced by sixteen Actinomycetes isolates showed anti-quorum sensing activity towards reporter stain Chromobacterium violaceum wild-type. Most of these extracts showed better inhibitory activity on all of the pathogenic bacteria biofilm structure tested than the destructive activity on the preformed of those biofilm structure. Subsequently, we also performed characterization of bioactive compound and found that in this study, polysaccharide is the most common antibiofilm agents, which were responsible to their antibiofilm activity. Finally, we found that the value of LC50 of all extracts tested were more than 1 mg/mL, thereby all of extracts tested did not show cyto-toxic effect against Artemia salina. Conclusion All of the extracts of Actinomycetes isolates showed promising inhibitory activity towards biofilm structure of pathogenic bacteria tested. So far, all of the extracts are potential to be QS inhibitors and antibiofilm agents of all pathogenic bacteria tested.

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