scholarly journals Occurrence of Anaplasma phagocytophilum in goats and sheep in Hebei Province, China

2019 ◽  
Vol 75 (11) ◽  
pp. 6344-2019
Author(s):  
QIUYUE WANG ◽  
YUAN LI ◽  
YAXUE ZOU ◽  
JIA LIU ◽  
LIFENG CHEN ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Genetic Characterization ◽  
Hebei Province ◽  
Emerging Pathogen ◽  
Granulocytic Anaplasmosis ◽  
16S Rna ◽  
Positive Rate ◽  
Epidemiologic Features ◽  
16S Rna Gene

Anaplasma phagocytophilum is an emerging pathogen known to cause human granulocytic anaplasmosis (HGA). Here we determined the prevalence and genetic characterization of A. phagocytophilum in Hebei Province, China. A total of 253 samples were taken from goats and sheep in Hebei Province, and 52 (20.6%) were positive for A. phagocytophilum. There was a higher positive rate in sheep (23.8%, 20/84) than in goats (18.9%, 32/169). Analysis of the partial 16S RNA gene sequences of A. phagocytophilum revealed that the isolates in this study were members of the same clade and were 100% homologous with each other. This study provides information on the epidemiologic features of A. phagocytophilum.

Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe

2013 ◽  
Vol 62 (12) ◽  
pp. 1891-1896 ◽  
Author(s):  
Beata Dzięgiel ◽  
Lukasz Adaszek ◽  
Mateusz Winiarczyk ◽  
Ignacio García-Bocanegra ◽  
Alfonso Carbonero ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Eastern Europe ◽  
Anaplasma Phagocytophilum ◽  
Genetic Material ◽  
Nucleotide Sequences ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Granulocytic Anaplasmosis ◽  
16S Rna ◽  
16S Rna Gene

The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagocytophilum was identified in the blood of 42 animals. The sequences of the 16S RNA gene amplicons that were obtained from our A. phagocytophilum isolates had 100 % similarity with each other and 96.4–100 % similarity with Anaplasma spp. sequences selected from those available in GenBank. Nucleotide substitutions at positions 248 and 249 were demonstrated in all the 16S RNA gene sequences of Anaplasma obtained in our study. This may indicate the emergence of a new rickettsial genotype that is the cause of equine granulocytic anaplasmosis in southern and eastern Europe. These results add new information on the epidemiology and genetic diversity of A. phagocytophilum detected in the blood of horses from southern and eastern Europe.

scholarly journals Genetic Characterization of Porcine Epidemic Diarrhea Virus in China Between 2014 and 2018: Emergence of the G1c Subtype

2020 ◽  
Vol 40 (04) ◽  
pp. 474-478
Author(s):  
Wenqiang Jiao
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Phylogenetic Trees ◽  
Genetic Characterization ◽  
Diagnostic Methods ◽  
Clinical Samples ◽  
Swine Industry ◽  
Diarrhea Virus ◽  
Positive Rate ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) has caused substantial economical loss to the Chinese swine industry. To illustrate the genetic characterization of PEDV circulating in China, 205 clinical samples between 2014 and 2018 were collected from 7 provinces in China. 93.17% (191 of 205) of the intestinal and fecal samples were positive for PEDV. 25 S1 amino acid (aa) together with 27 ORF3 genes from 8 provinces were sequenced and analyzed. The phylogenetic trees based on the S1 and ORF3 genes were constructed by the neighbor-joining method using MEGA 7 software. PEDV prevalence was 86.96% (40 of 46) of the swine farms in the 8 provinces and the PEDV positive rate was 93.17% (191 of 205) in the tested samples. Genetic analysis showed CH-JIANGXI-1-2016 CH-JIAGNXI-2-2016, CH-JIANGXI-3-2016 and CH-JIANGXI-2017 had three notable insertions or deletions occurred at aa 59-62, 160, and 139 (140) when compared to all of the strains in this study; moreover, phylogenetic analysis indicated that the four isolates formed a new branch significantly different from G1a, G1b and Indel subtype based on S1 gene: that is the G1c subtype. More research is needed to determine whether the insertions and deletions had biological influence on the virus. The results acquired in the present study showed the genetic diversity of PEDV circulating in 8 provinces, providing information for the development of new diagnostic methods and new vaccines

Characterization of hydrocortisone bioconversion and 16S RNA gene in Synechococcus nidulans cultures

2010 ◽  
Vol 46 (2) ◽  
pp. 191-197 ◽  
Author(s):  
S. Rasoul-Amini ◽  
Y. Ghasemi ◽  
M. H. Morowvat ◽  
M. B. Ghoshoon ◽  
M. J. Raee ◽  
...  
Keyword(s):  
16S Rna ◽  
16S Rna Gene

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

Clinical and genetic characterization of two cases of central hypogonadism in Klinefelter syndrome

2020 ◽  
Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Klinefelter Syndrome
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