scholarly journals Serological evidence of canine influenza virus infection in shelter dogs in Turkey

2018 ◽  
Vol 74 (6) ◽  
pp. 5986-2018 ◽  
Author(s):  
HAKAN AYDIN ◽  
AKIN KIRBAS ◽  
MEHMET OZKAN TIMURKAN ◽  
MUSTAFA SINAN AKTAS ◽  
GULIZAR ACAR KIRMIZI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Influenza Virus ◽  
Virus Infection ◽  
Reverse Transcriptase ◽  
Influenza Virus Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Canine Influenza Virus ◽  
Polymerase Chain ◽  
Canine Influenza

Influenza virus infection is an important disease which occurs in humans and a variety of animals. Because of the wide host adaptation and segmented genome, there is always the possibility of mutations and interspecies transmission of the influenza virus. Our study is the first to draw attention to canine influenza infection in Turkey. For this purpose, 208 sera and swab samples were collected from dogs with respiratory and nonrespiratory signs in various seasons. Out of the 208 dogs, 94 (45.2%) were male and 114 (54.8%) were female; the average age was 4.7 years. A total of 208 sera samples were tested for the presence of canine influenza virusspecific antibodies by the indirect enzyme-linked immunosorbent assay. The seroprevalence of canine influenza virus infection was 11/208 (5.8%). With regard to seasonal distribution, the highest rate of seropositivity was detected in spring, and the lowest in summer. Molecular detection of the canine influenza virus from nasal swab samples was done by reverse transcriptase polymerase chain reaction using specific universal primers for the hemagglutinin gene. Influenza virus nucleic acid could not be detected by reverse transcriptase polymerase chain reaction. In this study, we revealed for the first time the existence of the canine influenza virus in Turkey. Although the seroprevalence was relatively low, it would be useful to investigate the canine influenza virus on a large scale and among dogs with infectious respiratory disease in the Turkish dog population..

scholarly journals Serologic Reports of H3N2 Canine Influenza Virus Infection in Dogs in Northeast China

2013 ◽  
Vol 75 (8) ◽  
pp. 1061-1062 ◽  
Author(s):  
Yong-Biao ZHANG ◽  
Ji-Dang CHEN ◽  
Jie-Xiong XIE ◽  
Wan-Jun ZHU ◽  
Chun-Ya WEI ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Northeast China ◽  
Influenza Virus Infection ◽  
Canine Influenza Virus ◽  
Canine Influenza

scholarly journals Prevalence of canine parvo virus and canine influenza virus infection in dogs in Dhaka, Mymensingh, Feni and Chittagong districts of Bangladesh

2016 ◽  
Vol 2 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Soumitra Sen ◽  
Md Siddiqur Rahman ◽  
Minakshi Nag ◽  
Mohummad Muklesur Rahman ◽  
Roma Rani Sarker ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Influenza Virus Infection ◽  
Canine Parvovirus ◽  
Age Group ◽  
Pet Dogs ◽  
Canine Influenza Virus ◽  
Test Kit ◽  
Canine Influenza ◽  
Age And Sex

Canine parvovirus (CPV) and canine influenza virus (CIV) are highly contagious virus infecting members of the canine family including dogs, coyotes, foxes and wolves. The research work was carried out to determine the prevalence of CPV and CIV in dogs (stray and pet dogs) in Dhaka, Mymensingh, Feni and Chittagong Districts by using Rapigen CPV and CIV Ag test kit. Fecal samples were collected from 50 dogs of different age and sex from different spots of Dhaka and Chittagong District. Nasal fluid samples were collected from 50 dogs of different age and sex from different spots. The samples were tested with Rapigen canine parvovirus Ag test kit and Rapigen canine influenza Ag test kit. Overall prevalence of CPV in pet dogs was 22% and in stray dogs was 30%. The prevalence of canine parvovirus in relation to age was gradually decreasing with higher age group and the prevalence was 28% in 1-6 months age, 16.66% in 7-12 months age group 11.11%o ver 18 months age group. The prevalence of canine parvovirus was significantly higher in male (24.13%) than that in female (19.04%). All samples showed negative test result for canine influenza virus. Prevalence of canine influenza virus infection were 00.00% in all age groups and sex. Overall prevalence of CIV in all pet and stray dogs were 00.00%.Asian J. Med. Biol. Res. March 2016, 2(1): 138-142

Serological evidence of H3N2 canine influenza virus infection among horses with dog exposure

2019 ◽  
Vol 66 (2) ◽  
pp. 915-920
Author(s):  
Pei Zhou ◽  
Aijian Luo ◽  
Xiangyu Xiao ◽  
Xue Hu ◽  
Dan Shen ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Influenza Virus Infection ◽  
Serological Evidence ◽  
Canine Influenza Virus ◽  
Canine Influenza

scholarly journals Bluetongue Virus Detection: A Safer Reverse-Transcriptase Polymerase Chain Reaction for Prediction of Viremia in Sheep

1997 ◽  
Vol 9 (2) ◽  
pp. 118-124 ◽  
Author(s):  
G. Shad ◽  
W. C. Wilson ◽  
J. O. Mecham ◽  
J. F. Evermann
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Bluetongue Virus ◽  
Virus Isolation ◽  
Extraction Procedure ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Antigen Capture ◽  
Virus Serotype ◽  
Polymerase Chain

A reversible target capture viral RNA extraction procedure was combined with a reverse-transcriptase nested polymerase chain reaction (PCR) to develop a capture PCR assay providing a rapid and safe prediction method for circulating bluetongue virus in infected ruminants. This new assay was compared with virus isolation and a recently developed antigen-capture enzyme-linked immunosorbent assay (ELISA) for the detection of bluetongue virus. Eight Warhill crossbred sheep were inoculated subcutaneously with bluetongue virus serotype 10, and blood samples were taken sequentially over a period of 28 days. The capture PCR detected the peak of viremia, as determined by virus isolation and antigen-capture ELISA, from day 5 to day 14 after challenge. The results indicate that the rapid-capture bluetongue virus PCR provides a rapid indicator of samples in which virus can be isolated. In addition, this capture bluetongue virus PCR procedure does not require a lengthy phenol extraction or the use of the highly toxic methyl mercury hydroxide denaturant.

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