scholarly journals Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin

2017 ◽  
Vol 73 (9) ◽  
pp. 585-590
Author(s):  
Michał Dąbrowski ◽  
Łukasz Zielonka ◽  
Mirosław Baranowski ◽  
Magdalena Gajęcka ◽  
Anna Rykaczewska ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Low Dose ◽  
Lymphocyte Subsets ◽  
Lymphocyte Subpopulations ◽  
T Lymphocyte Subsets ◽  
T Lymphocyte Subpopulations ◽  
Small Doses ◽  
And Control ◽  
Average Body ◽  
Average Body Weight

Most plant food products and feed ingredients can be contaminated with small doses of fusarial mycotoxins, which cause subclinical changes in humans and animals. The purpose of this study has been to evaluate the effect of low doses of zearalenone (40 µg/kg BW) and deoxynivalenol (12 µg/kg BW) administered daily per os to gilts on T-lymphocyte subpopulations (CD4+CD8-, CD4+CD8+, CD4-CD8+) in mesenteric blood during six-week exposure. The experiment was conducted on 36 gilts with an average body weight of 25±2 kg, divided into two groups: experimental (E – which received ZEN + DON) and control (C – which received a placebo). Changes in percentages of particular T-lymphocyte subsets were assessed by flow cytometry. Blood samples were taken at regular weekly intervals from 6 gilts during laparotomy, immediately before the heart’s action ceased. The analyses demonstrated that the E group had a transient decrease in the percentage of T-lymphocytes CD4+CD8+, as well as some disturbance in the linear correlation of growth within the same population of lymphocytes. Mixed low-dose mycotoxin can also be a cause of temporary immunity decline, as well as a factor responsible for disturbances during the maturation of the immune system.

Effect of low-dose interleukin-2 and interferon alfa on t-lymphocyte subsets in melanoma

Nutrition ◽  
1996 ◽  
Vol 12 (11-12) ◽  
pp. 845
Author(s):  
Eva Burešová ◽  
Pavel Živný ◽  
Jan Krejsek ◽  
Otakar Kopecký ◽  
Doris Vokurková ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Low Dose ◽  
Lymphocyte Subsets ◽  
Interleukin 2 ◽  
Interferon Alfa ◽  
T Lymphocyte Subsets

scholarly journals The absolute counts of peripheral T lymphocyte subsets in patient with ankylosing spondylitis and the effect of low-dose interleukin-2

Medicine ◽  
2019 ◽  
Vol 98 (15) ◽  
pp. e15094 ◽  
Author(s):  
Haizhuan An ◽  
Xin Li ◽  
Fang Li ◽  
Chong Gao ◽  
Xiaofeng Li ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
T Lymphocyte ◽  
Low Dose ◽  
Lymphocyte Subsets ◽  
Interleukin 2 ◽  
T Lymphocyte Subsets ◽  
The Absolute

T-lymphocyte subsets and thymic size in malnourished infants in Egypt: a hospital-based study

2007 ◽  
Vol 13 (5) ◽  
pp. 1031-1042 ◽  
Author(s):  
M.F. Nassar ◽  
N.T. Younis ◽  
A.G. Tohamy ◽  
D.M. Dalam ◽  
M.A. El Badawy
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets

scholarly journals Flow cytometric analysis of T lymphocytes and cytokines in aqueous humor of patients with varicella zoster virus-mediated acute retinal necrosis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hao Kang ◽  
Yunbo Wei ◽  
Ming Liu ◽  
Di Yu ◽  
Yong Tao
Keyword(s):  
T Lymphocytes ◽  
Varicella Zoster Virus ◽  
Aqueous Humor ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Flow Cytometric Analysis ◽  
T Lymphocyte Subsets ◽  
Varicella Zoster ◽  
Flow Cytometric ◽  
Cd8 T Lymphocytes

Abstract Background The purpose of this study is to investigate the aqueous humor (AH) T lymphocyte subsets and cytokines of acute retinal necrosis (ARN) to elucidate the immunologic inflammatory features of this disorder. Methods Three patients with ARN infected with varicella zoster virus (VZV) who underwent multiple intravitreal injections of ganciclovir were enrolled in this study. The control group consisted of four non-infectious patients with acute anterior uveitis (AAU). Flow cytometric analysis was performed on the lymphocyte subsets from the AH and peripheral blood (PB) samples during the active phase of intraocular inflammation. Five inflammatory cytokines were measured in each AH sample and various clinical characteristics were also assessed. Results VZV deoxyribonucleic acid (DNA) was detected by real-time polymerase chain reaction (PCR) in AH from all the ARN patients, who showed higher CD8+ T lymphocytes population in AH than the AAU patients (p = 0.006). CD4/CD8 ratios of T lymphocytes and the percentage of CD8 + CD25+ T lymphocytes in AH were significantly lower in ARN than in AAU (p = 0.006; p = 0.012). In the ARN patients, the percentages of CD4+ and CD8+ T lymphocytes in AH were higher than those found in PB. The percentage of CD4 + CD25+ T lymphocytes in AH was significantly higher than the proportion in PB in the AAU patients (p = 0.001). Immunoregulatory cytokine Interleukin-10 in AH was significantly elevated in the ARN patients in comparison with the case of the AAU patients (p = 0.036). In ARN, the copy number of VZV DNA in AH positively correlated with the percentage of CD8+ T lymphocytes in AH and negatively correlated with the CD4/CD8 ratio in AH during the course of disease treatment (p = 0.009, r = 0.92; p = 0.039, r = − 0.834). Conclusion The ARN patients caused by VZV had different intraocular T lymphocyte subsets and cytokines profile than those of the non-infectious patients. High percentages of CD8+ T lymphocytes and low CD4/CD8 T cell ratios may be a potential biomarker for diagnosis of viral-infectious uveitis. T lymphocytes examination at the inflammatory sites has the potential to become a useful research tool for differentiating viral and non-viral uveitis.

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