scholarly journals Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation

2021 ◽  
Vol 68 (3.4) ◽  
pp. 347-353
Author(s):  
Shuhai Chen ◽  
Tetsuya Ikemoto ◽  
Takuya Tokunaga ◽  
Shohei Okikawa ◽  
Katsuki Miyazaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Folic Acid ◽  
Folic Acid Supplementation ◽  
Adipose Derived Stem Cells ◽  
Acid Supplementation

Folate: In Vitro and in Vivo Effects on VLDL and LDL Oxidation

2007 ◽  
Vol 77 (1) ◽  
pp. 66-72 ◽  
Author(s):  
McEneny ◽  
Couston ◽  
McKibben ◽  
Young ◽  
Woodside
Keyword(s):  
Folic Acid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Folic Acid Supplementation ◽  
Serum Folate ◽  
Ldl Oxidation ◽  
Low Density ◽  
Acid Supplementation

Raised total homocysteine (tHcy) levels may be involved in the etiology of cardiovascular disease and can lead to damage of vascular endothelial cells and arterial wall matrix. Folic acid supplementation can help negate these detrimental effects by reducing tHcy. Recent evidence has suggested an additional anti-atherogenic property of folate in protecting lipoproteins against oxidation. This study utilized both an in vitro and in vivo approach. In vitro: Very-low-density lipoprotein (VLDL) and low density lipoprotein (LDL) were isolated by rapid ultracentrifugation and then oxidized in the presence of increasing concentrations (0→ μmol/L) of either folic acid or 5-methyltetrahydrofolate (5-MTHF). In vivo: Twelve female subjects were supplemented with folic acid (1 mg/day), and the pre- and post-VLDL and LDL isolates subjected to oxidation. In vitro: 5-MTHF, but not folic acid, significantly increased the resistance of VLDL and LDL to oxidation. In vivo: Following folic acid supplementation, tHcy decreased, serum folate increased, and both VLDL and LDL displayed a significant increase in their resistance to oxidation. These results indicated that in vitro, only the active form of folate, 5-MTHF, had antioxidant properties. In vivo results demonstrated that folic acid supplementation reduced tHcy and protected both VLDL and LDL against oxidation. These findings provide further support for the use of folic acid supplements to aid in the prevention of atherosclerosis.

Folic acid supplementation affects apoptosis and differentiation of embryonic neural stem cells exposed to high glucose

2008 ◽  
Vol 440 (1) ◽  
pp. 27-31 ◽  
Author(s):  
De-yong Jia ◽  
Hui-juan Liu ◽  
Fu-wu Wang ◽  
Shang-ming Liu ◽  
Eng-Ang Ling ◽  
...  
Keyword(s):  
Stem Cells ◽  
Folic Acid ◽  
Neural Stem Cells ◽  
High Glucose ◽  
Folic Acid Supplementation ◽  
Acid Supplementation

Folic Acid Supplementation Modifies β-Adrenoceptor–Mediated In Vitro Lipolysis of Obese/Diabetic (+db/+db) Mice

2009 ◽  
Vol 234 (9) ◽  
pp. 1047-1055 ◽  
Author(s):  
Tsz Yan Lam ◽  
Sai Wang Seto ◽  
Alice Lai Shan Au ◽  
Christina Chui Wa Poon ◽  
Rachel Wai Sum Li ◽  
...  
Keyword(s):  
Folic Acid ◽  
Protein Expression ◽  
Folic Acid Supplementation ◽  
Adipose Tissues ◽  
Acid Supplementation ◽  
Acid Consumption ◽  
Cgp 12177 ◽  
Dose Dependent Increase ◽  
Dose Dependent

The effects of folic acid (5.7 and 71 μg/kg, 4 weeks) consumption on the β-adrenoceptors (β-ARs)–elicited lipolysis in vitro of the abdominal adipocytes of lean/control (+ m/+ db) and obese/diabetic (+ db/+ db) mice (female) were investigated. β-AR agonists (salbutamol, a β2-AR agonist; BRL 37344 and CGP 12177, β3-AR agonists; adrenaline, a β-AR agonist)–mediated lipolysis, β2-, and β3-ARs protein expression of the adipose tissues after folic acid consumption were evaluated. Our results demonstrate that a smaller magnitude of the basal (spontaneous) and the β-AR agonists–triggered lipolysis was observed in + db/+ db mice, and folic acid supplementation (71 μg/kg) resulted in an improvement of both the baseline and the β-ARs–mediated lipolysis. In controls, a lower β2-and β3-ARs protein expression of the adipose tissues was detected in + db/+ db mice, compared to + m/+ db mice. In both strains fed with folic acid (71 μg/kg), a reduction of β2-AR protein expression was observed compared to the respective controls. In + db/+ db mice, folic acid (5.7 and 71 μg/kg) consumption caused a dose-dependent increase of β3-AR protein expression compared to controls. We demonstrate that lipolysis elicited by β-AR (β2- and β3-ARs) agonists was blunted in + db/+ db mice. Folic acid consumption has significant modulatory effects on β-ARs protein expression and lipolysis.

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