scholarly journals Role of serine 249 of ezrin in the regulation of sodium-dependent phosphate transporter NaPi-IIa activity in renal proximal tubular cells

2013 ◽  
Vol 60 (1.2) ◽  
pp. 27-34 ◽  
Author(s):  
Fumiyo Yamada ◽  
Daisuke Horie ◽  
Asako Nakamura ◽  
Ayako Tanimura ◽  
Hironori Yamamoto ◽  
...  
Keyword(s):  
Phosphate Transporter ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Sodium Dependent ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular

Regulation of renal sodium-dependent phosphate co-transporter genes (Npt2a and Npt2c) by all-trans-retinoic acid and its receptors

2010 ◽  
Vol 429 (3) ◽  
pp. 583-592 ◽  
Author(s):  
Masashi Masuda ◽  
Hironori Yamamoto ◽  
Mina Kozai ◽  
Sarasa Tanaka ◽  
Mariko Ishiguro ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Sodium Dependent ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular

The type II sodium-dependent phosphate co-transporters Npt2a and Npt2c play critical roles in the reabsorption of Pi by renal proximal tubular cells. The vitamin A metabolite ATRA (all-trans-retinoic acid) is important for development, cell proliferation and differentiation, and bone formation. It has been reported that ATRA increases the rate of Pi transport in renal proximal tubular cells. However, the molecular mechanism is still unknown. In the present study, we observed the effects of a VAD (vitamin A-deficient) diet on Pi homoeostasis and the expression of Npt2a and Npt2c genes in rat kidney. There was no change in the plasma levels of Pi, but VAD rats significantly increased renal Pi excretion. Renal brush-border membrane Pi uptake activity and renal Npt2a and Npt2c expressions were significantly decreased in VAD rats. The transcriptional activity of a luciferase reporter plasmid containing the promoter region of human Npt2a and Npt2c genes was increased markedly by ATRA and a RAR (retinoic acid receptor)-specific analogue TTNPB {4-[E-2-(5,6,7,8-tetrahydro-5,5,8,8-tetra-methyl-2-naphtalenyl)-1-propenyl] benzoic acid} in renal proximal tubular cells overexpressing RARs and RXRs (retinoid X receptors). Furthermore, we identified RAREs (retinoic acid-response elements) in both gene promoters. Interestingly, the half-site sequences (5′-GGTTCA-3′: −563 to −558) of 2c-RARE1 overlapped the vitamin D-responsive element in the human Npt2c gene and were functionally important motifs for transcriptional regulation of human Npt2c by ATRA and 1,25(OH)2D3 (1α,25-dihydroxyvitamin D3), in both independent or additive actions. In summary, we conclude that VAD induces hyperphosphaturia through the down-regulation of Npt2a and Npt2c gene expression in the kidney.

scholarly journals Role of P-Glycoprotein Expression and Function in Cystinotic Renal Proximal Tubular Cells

Pharmaceutics ◽  
2011 ◽  
Vol 3 (4) ◽  
pp. 782-792 ◽  
Author(s):  
Karen Peeters ◽  
Martijn J. Wilmer ◽  
Joost P. Schoeber ◽  
Dorien Reijnders ◽  
Lambertus P. van den Heuvel ◽  
...  
Keyword(s):  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
P Glycoprotein ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular ◽  
And Function

scholarly journals Sodium-dependent phosphate cotransporter type 1 sequence polymorphisms in male patients with gout

2009 ◽  
Vol 69 (6) ◽  
pp. 1232-1234 ◽  
Author(s):  
Wako Urano ◽  
Atsuo Taniguchi ◽  
Naohiko Anzai ◽  
Eisuke Inoue ◽  
Yoshikatsu Kanai ◽  
...  
Keyword(s):  
Nucleotide Polymorphisms ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Sodium Dependent ◽  
Proximal Tubular ◽  
Male Patients ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular

ObjectivesMolecular biological approaches have recently identified urate transporters in renal proximal tubular cells. Human sodium-dependent phosphate cotransporter type 1 encoded by SLC17A1 is a urate transporter localised to the renal proximal tubular cells and candidate molecule to secret urate from renal tubular cells to urine. This study investigated the roles of SLC17A1 in the development of gout.Patients and MethodsSingle nucleotide polymorphisms in the human SLC17A1 gene (rs1165176, rs1165151, rs1165153, rs1165196, rs1165209, rs1165215, rs1179086, rs3799344 and rs3757131) were selected, and an association study was conducted using male patients with gout (n=175) and male controls (n=595).ResultsThere were significant differences between gout and control groups in the distribution of genotypes at rs1165196 (T806C; Ile269Thr, odds ratio (OR) 0.55, p=0.0035), rs1179086 (OR 0.57, p=0.0018) and rs3757131 (OR 0.54, p=0.0026). In controls, T806C alone had no effect on serum uric acid (sUA) levels. However, T806C showed significant interaction with a reduction of sUA in obese individuals (body mass index ≥25) using multiple regression analysis.ConclusionsOur data suggest that SLC17A1 polymorphisms are associated with the development of gout.

Role of metabolites in MDMA (ecstasy)-induced nephrotoxicity: an in vitro study using rat and human renal proximal tubular cells

2002 ◽  
Vol 76 (10) ◽  
pp. 581-588 ◽  
Author(s):  
Márcia Carvalho ◽  
Gabrielle Hawksworth ◽  
Nuno Milhazes ◽  
Fernanda Borges ◽  
Terrence Monks ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular
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