Growth ability in simian cells of monkey cell-tropic HIV-1 is greatly affected by downstream region of the vif gene

Tomoki Yamashita; Naoya Doi; Akio Adachi; Masako Nomaguchi

doi:10.2152/jmi.55.236

Growth ability in simian cells of monkey cell-tropic HIV-1 is greatly affected by downstream region of the vif gene

The Journal of Medical Investigation ◽

10.2152/jmi.55.236 ◽

2008 ◽

Vol 55 (3,4) ◽

pp. 236-240 ◽

Cited By ~ 8

Author(s):

Tomoki Yamashita ◽

Naoya Doi ◽

Akio Adachi ◽

Masako Nomaguchi

Keyword(s):

Monkey Cell ◽

Downstream Region ◽

Hiv 1 ◽

Growth Ability

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Replication potentials of vif variant viruses generated from monkey cell-tropic HIV-1 derivative clones NL-DT5/NL-DT5R

Microbes and Infection ◽

10.1016/j.micinf.2008.06.007 ◽

2008 ◽

Vol 10 (10-11) ◽

pp. 1218-1222 ◽

Cited By ~ 2

Author(s):

K HATCHO ◽

K KAMADA ◽

T YAMASHITA ◽

A ADACHI ◽

M NOMAGUCHI

Keyword(s):

Monkey Cell ◽

Hiv 1

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Production of Immunogenic HIV-1 Viruslike Particles in Stably Engineered Monkey Cell Lines

AIDS Research and Human Retroviruses ◽

10.1089/aid.1991.7.17 ◽

1991 ◽

Vol 7 (1) ◽

pp. 17-27 ◽

Cited By ~ 25

Author(s):

JOEL R. HAYNES ◽

SHI XIAN CAO ◽

BENJAMIN ROVINSKI ◽

CHARLES SIA ◽

OLIVE JAMES ◽

...

Keyword(s):

Cell Lines ◽

Monkey Cell ◽

Viruslike Particles ◽

Hiv 1

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Growth ability in various macaque cell lines of HIV-1 with simian cell-tropism

The Journal of Medical Investigation ◽

10.2152/jmi.57.284 ◽

2010 ◽

Vol 57 (3,4) ◽

pp. 284-292 ◽

Cited By ~ 6

Author(s):

Naoya Doi ◽

Sachi Fujiwara ◽

Akio Adachi ◽

Masako Nomaguchi

Keyword(s):

Cell Lines ◽

Cell Tropism ◽

Hiv 1 ◽

Simian Cell ◽

Growth Ability

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Ultrastructural observations of human monocytes infected with HIV-1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084843 ◽

1991 ◽

Vol 49 ◽

pp. 108-109

Author(s):

James K. Koehler ◽

Steven G. Reed ◽

Joao S. Silva

Keyword(s):

Gradient Centrifugation ◽

Virus Production ◽

Human Monocyte ◽

Red Cross ◽

Human Monocytes ◽

Blood Monocytes ◽

Hiv Replication ◽

Electron Microscopic ◽

Ultrastructural Studies ◽

Hiv 1

As part of a larger study involving the co-infection of human monocyte cultures with HIV and protozoan parasites, electron microscopic observations were made on the course of HIV replication and infection in these cells. Although several ultrastructural studies of the cytopathology associated with HIV infection have appeared, few studies have shown the details of virus production in “normal,” human monocytes/macrophages, one of the natural targets of the virus, and suspected of being a locus of quiescent virus during its long latent period. In this report, we detail some of the interactions of developing virons with the membranes and organelles of the monocyte host.Peripheral blood monocytes were prepared from buffy coats (Portland Red Cross) by Percoll gradient centrifugation, followed by adherence to cover slips. 90-95% pure monocytes were cultured in RPMI with 5% non-activated human AB serum for four days and infected with 100 TCID50/ml of HIV-1 for four hours, washed and incubated in fresh medium for 14 days.

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