Microbicidal activity of hydrolyzed toxoplasma immune mouse serum (HS-LKs) in heterologous cell cultures.

1982 ◽  
Vol 10 (3/4) ◽  
pp. 183-195 ◽  
Author(s):  
HARUHISA SAKURAI ◽  
MOTOYOSHI SATO ◽  
TSUNEO HIROSE ◽  
YOSHITAKA OMATA ◽  
ATSUSHI SAITO ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Mouse Serum ◽  
Immune Mouse ◽  
Microbicidal Activity ◽  
Heterologous Cell ◽  
Immune Mouse Serum

Resistance of Trypanosoma cruzi to Blood Clearance Induced by Acute-Phase Immune Mouse Serum

1992 ◽  
Vol 78 (6) ◽  
pp. 1074
Author(s):  
José M. Alvarez ◽  
Claudio Romero Farias Marinho ◽  
Ayumi Oshima ◽  
Liliane Guimaraes ◽  
Hércules Menezes ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Acute Phase ◽  
Mouse Serum ◽  
Blood Clearance ◽  
Immune Mouse ◽  
Immune Mouse Serum

Stage-specific proteins ofPlasmodium berghei-infected red blood cells detected by antibodies of immune mouse serum

1988 ◽  
Vol 75 (1) ◽  
pp. 69-72 ◽  
Author(s):  
T. P. M. Schetters ◽  
C. C. Hermsen ◽  
A. A. J. C. Van Zon ◽  
W. M. C. Eling
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mouse Serum ◽  
Immune Mouse ◽  
Specific Proteins ◽  
Immune Mouse Serum

scholarly journals Candidacidal activity of mouse macrophages in vitro

1980 ◽  
Vol 29 (2) ◽  
pp. 477-482 ◽  
Author(s):  
P K Maiti ◽  
R Kumar ◽  
L N Mohapatra
Keyword(s):  
Peritoneal Macrophages ◽  
Mouse Serum ◽  
Activated Macrophages ◽  
Immune Mouse ◽  
Mouse Macrophages ◽  
In Vitro Exposure ◽  
Candida Infections ◽  
Mouse Peritoneal Macrophages ◽  
Immune Mouse Serum

Mouse peritoneal macrophages were infected in vitro with Candida albicans, and the phagocytic and candidacidal activities were estimated by microscopic examination of Giemsa-stained cells. Activated macrophages obtained from either BCG-vaccinated animals or by in vitro exposure of normal macrophages to phytohemagglutinin-induced lymphokines exhibited higher phagocytic and candidacidal activities than did normal macrophages. However, activated macrophages obtained by in vitro exposure of macrophages to candida-induced lymphokines exhibited the highest phagocytic and candidacidal activities. The incorporation of immune mouse serum into the culture medium also enhanced the phagocytic and candidacidal activities of the normal macrophages but failed to improve the function of the activated macrophages. These results suggest that both activated macrophages and antibodies may be required for controlling candida infections in mice.

scholarly journals THE PASSIVE TRANSFER OF ACQUIRED RESISTANCE TO LISTERIA MONOCYTOGENES

1964 ◽  
Vol 120 (1) ◽  
pp. 93-103 ◽  
Author(s):  
K. Miki ◽  
G. B. Mackaness
Keyword(s):  
Listeria Monocytogenes ◽  
Acquired Resistance ◽  
Survival Rates ◽  
Peritoneal Macrophages ◽  
Passive Transfer ◽  
Mouse Serum ◽  
Immune Mouse ◽  
Passive Protection ◽  
Mouse Macrophages ◽  
Immune Mouse Serum

Five methods have been used in an effort to reveal an antibody that could account for the features of acquired resistance to Listeria monocytogenes: (a) a comparison of the growth rates of Listeria in the spleens of mice infused repeatedly with normal or immune mouse serum; (b) measurement of peritoneal clearance of Listeria in the presence of normal or immune mouse serum; (c) the survival rate of Listeria in monolayers of mouse macrophages infected in the presence of normal or immune mouse serum; (d) the effect of injecting urea extracts of spleens and peritoneal macrophages of normal or immune mice on the survival and growth of Listeria in recipient animals; (e) a comparison of survival rates in lethally infected mice following the passive transfer of cells and serum from normal or immune donors. The only evidence of passive protection was obtained when intact living cells from immune donors were used for transfer under conditions which permitted them to interact with the parasite population.

Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects--incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme.

1986 ◽  
Vol 32 (3) ◽  
pp. 476-481 ◽  
Author(s):  
R J Thompson ◽  
A P Jackson ◽  
N Langlois
Keyword(s):  
Blood Donors ◽  
Solid Phase ◽  
Normal Subjects ◽  
Rabbit Serum ◽  
Mouse Serum ◽  
Serum Samples ◽  
Immune Mouse ◽  
Creatine Kinase Mb ◽  
Circulating Antibodies ◽  
Immune Mouse Serum

Abstract With a two-site CK-MB assay, we screened serum samples from 1008 blood donors for the presence of antibodies to mouse monoclonal immunoglobulin. These antibodies were capable of cross-linking the labeled antibody with the solid-phase antibody in the two-site assay, thus generating a falsely high apparent CK-MB concentration. In 92 (9.12%) of the blood donors tested, apparent CK-MB concentrations of 10-1000 micrograms/L decreased to less than 3 micrograms/L when re-assayed with non-immune mouse serum (10 mL/L) included in the assay reagent. We tested the ability of non-immune sera from other animal species to lower the concentration of apparent CK-MB in 58 of the 92 samples. Bovine and ovine serum were almost as effective as mouse serum; feline, canine, and rabbit serum were less effective. Of the samples tested, 12% (1.1% of the original population screened) showed apparent CK-MB values that either were not depressed by bovine serum or were only partly depressed. We discuss the possible etiology of these antibodies in normal subjects and recommend that all mouse monoclonal two-site assays should contain non-immune mouse serum (or a suitable "irrelevant" mouse monoclonal antibody) to prevent false-positive results.

scholarly journals Trypanosoma cruzi: circulating antigens

1981 ◽  
Vol 76 (1) ◽  
pp. 71-82 ◽  
Author(s):  
V. Bongertz ◽  
Klaus-Dieter Hungerer ◽  
Bernardo Galvão-Castro
Keyword(s):  
Trypanosoma Cruzi ◽  
Infected Cell ◽  
Cell Cultures ◽  
Culture Medium ◽  
Mouse Serum ◽  
Circulating Antigens

Circulating antigens were detected in sera of mice experimentally infected with a high close of Trypanosoma cruzi by reaction with sera from chronically infected mice. The immunodiffusion reaction between homologous acute and chronic sera produced four precipitation lines. By reaction with chronic mouse serum, circulating antingens were detected in sera from heavily infected hamsters, dogs, rabbits and in sera from chagasic patients. A reaction was also found in urine from acutely infected mice and dogs. Trypanosoma cruzi exoantigen was detected in trypanosome culture medium and in the supernatant of infected cell cultures. Attempts to isolate the antigens are described.

scholarly journals Intranasal Administration of an Inactivated Yersinia pestis Vaccine with Interleukin-12 Generates Protective Immunity against Pneumonic Plague

2011 ◽  
Vol 18 (11) ◽  
pp. 1925-1935 ◽  
Author(s):  
Devender Kumar ◽  
Girish Kirimanjeswara ◽  
Dennis W. Metzger
Keyword(s):  
Yersinia Pestis ◽  
Protective Efficacy ◽  
Bacterial Colonization ◽  
Lethal Dose ◽  
Interleukin 12 ◽  
Cell Vaccine ◽  
Mouse Serum ◽  
Immune Mouse ◽  
Pneumonic Plague ◽  
Content Type

ABSTRACTInhalation ofYersinia pestiscauses pneumonic plague, which rapidly progresses to death. A previously licensed killed whole-cell vaccine is presently unavailable due to its reactogenicity and inconclusive evidence of efficacy. The present study now shows that vaccination intranasally (i.n.) with inactivatedY. pestisCO92 (iYp) adjuvanted with interleukin-12 (IL-12) followed by an i.n. challenge with a lethal dose ofY. pestisCO92 prevented bacterial colonization and protected 100% of mice from pneumonic plague. Survival of the vaccinated mice correlated with levels of systemic and lung antibodies, reduced pulmonary pathology and proinflammatory cytokines, and the presence of lung lymphoid cell aggregates. Protection against pneumonic plague was partially dependent upon Fc receptors and could be transferred to naïve mice with immune mouse serum. On the other hand, protection was not dependent upon complement, and following vaccination, depletion of CD4 and/or CD8 T cells before challenge did not affect survival. In summary, the results demonstrate the safety, immunogenicity, and protective efficacy of i.n. administered iYp plus IL-12 in a mouse model of pneumonic plague.

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