ANTIMICROBIAL RESISTANCE PATTERN AMONG NON-FERMENTING GRAM NEGATIVE BACILLI ISOLATED FROM CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL IN INDIA.

Increasing antimicrobial resistance is a worldwide concern. The prevalence of resistance among hospitalized patients varies in different location. The right choice of antibiotic is utmost importance to initiate empirical therapy especially in critical care areas. To compare and assess the differences in the pattern of antimicrobial resistance shown by Gram Negative Bacilli (GNB) isolates from general wards and Intensive Care Unit (ICU) patients. This is a retrospective study conducted in a tertiary care hospital on 100 (50 + 50) isolates of GNB from clinical samples collected from General ward and ICU patients respectively. GNB isolates were identified by standard biochemical tests and their antimicrobial susceptibility pattern was determined as per CLSI guidelines and analyzed for both the groups. The resistant strains were identified for Extended Spectrum Beta Lactamases (ESBL) and Metallo Beta Lactamase (MBL) production. In general ward isolates, Enterobacteriaceae was the commonest (78%) and nonfermenters accounts to 22%. In ICU, Enterobacteriaceae 64% and nonfermenters 36%. In ICU maximum resistance to third generation cephalosporins and fluoroquinolones was noted among nonfermenters. In contrary, in general ward, Escherichia coli showed highest resistance to almost all the drugs except aminoglycosides. Majority of the isolates in both the groups were sensitive to aminoglycosides (80%). ESBL producer in ICU was 80% and in general ward 72%. MBL production among nonfermenters in ICU was 25%. This study provides information on antibiotic resistance in different areas of the hospital. Need of the day is that, each hospital should have a comprehensively drafted and strictly implemented antibiotic policy.

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Prevalence of multidrug-resistant and extended-spectrum beta-lactamase producing Gram-negative isolates from clinical samples in a tertiary care hospital of Nepal

Tropical Medicine and Health ◽

10.1186/s41182-021-00313-3 ◽

2021 ◽

Vol 49 (1) ◽

Author(s):

Aryatara Shilpakar ◽

Mehraj Ansari ◽

Kul Raj Rai ◽

Ganesh Rai ◽

Shiba Kumar Rai

Keyword(s):

Tertiary Care Hospital ◽

Tertiary Care ◽

Polymyxin B ◽

Multidrug Resistant ◽

Confirmatory Test ◽

Clinical Samples ◽

Care Hospital ◽

Gram Negative ◽

Extended Spectrum ◽

Esbl Producers

Abstract Background The existence of multidrug-resistant organisms, including extended-spectrum beta-lactamases (ESBLs), is on rise across the globe and is becoming a severe problem. Knowledge of the prevalence and antibiogram profile of such isolates is essential to develop an appropriate treatment methodology. This study aimed to study the prevalence of Gram-negative isolates exhibiting ESBL at a tertiary care hospital and study their antibiogram profile. Methods A cross-sectional study was conducted at Shahid Gangalal National Heart Centre, Kathmandu, Nepal, from June 2018 to November 2018. A total of 770 clinical samples were collected and identified using the conventional biochemical tests following the Clinical and Laboratory Standard Institute (CLSI) guidelines. Antimicrobial susceptibility testing (AST) was performed using the standardized Kirby-Bauer disk diffusion method. The screening test for ESBL producers was performed as recommended by the CLSI and the confirmatory test was performed phenotypically using the E-test. Results Out of the 92 isolates, 84 (91.3%) were multidrug-resistant, and 47 (51.1%) were found to be potential ESBL producers. Of these, 16 isolates were confirmed ESBL producers by the E-test. Escherichia coli and Klebsiella pneumoniae were the predominant isolates and were also the major ESBL producers. Besides polymyxin B (100% sensitive), meropenem and imipenem showed high efficacy against the ESBL producers. Conclusion Multidrug resistance was very high; however, ESBL production was low. Polymyxin B and carbapenems are the choice of drugs against ESBL producers but should be used only as the last line drugs.

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A retrospective study of Antimicrobial resistance pattern of uropathogens in a Tertiary Care Hospital in Upper Assam

Indian Journal of Medical Microbiology ◽

10.1016/j.ijmmb.2021.08.022 ◽

2021 ◽

Vol 39 ◽

pp. S6

Author(s):

Anindita Patowary ◽

Atanu Chakraborty ◽

Uttara Borkotoki ◽

Purnima Barua

Keyword(s):

Retrospective Study ◽

Antimicrobial Resistance ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Resistance Pattern ◽

Care Hospital

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Antimicrobial Sensitivity Pattern in the Mixed Intensive Care Unit in a Tertiary Care Hospital of Eastern Nepal

Eastern Green Neurosurgery ◽

10.3126/egn.v2i2.29237 ◽

2020 ◽

Vol 2 (2) ◽

pp. 9-15

Author(s):

Niraj Kumar Keyal ◽

Mahendra Shrestha ◽

Partima Sigdel Ghimire

Keyword(s):

Intensive Care Unit ◽

Staphylococcus Aureus ◽

Intensive Care ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Polymyxin B ◽

Clinical Samples ◽

Care Hospital ◽

Gram Negative ◽

Sensitivity Pattern

Background: Empirical antibiotics are used in the intensive care unit based on developing countries’ guidelines due to a lack of a bacteriological profile of individual ICU and institution policy. Therefore, this study was conducted to know the antibiogram of the intensive care unit and to make institution policy for antibiotic use in ICU. Materials and methods: It was a prospective descriptive cross-sectional study conducted in the mixed surgical and medical intensive care unit of a tertiary care hospital for one year in 625 patients. Various clinical samples were collected aseptically and organisms were identified by the cultural characteristics, morphology, gram stain, and different biochemical test. Antimicrobial susceptibility was done with a disc diffusion test. Data collection was done in a preformed sheet that included all tested antibiotic and demographic variables. Statistical analysis was done by using statistical package for the social sciences. The result was presented as frequency and percentage. Results: Out of 625 samples, 135(22%) showed growth in culture. Among them, 96(71%) and 39(29%) were gram-negative bacilli and gram-positive cocci respectively. The tracheal aspirate was the most common type of specimen which comprised 49(36.29%) isolates. The most common organism was Staphylococcus aureus which accounts for 27(20%) isolates, followed by Acinetobacter baumanni 25(18.51%), Klebsiella pneumoniae 22(16.29%) and Pseudomonas aeurignosa 21(15.55%). The incidence of multidrug-resistant and extended drug resistance was 44(32.5%) and 45(33%) respectively. Meanwhile, the incidence of methicillin-resistant staphylococcus aureus was 70%. However, in the case of Acinetobacter baumannii and Enterobacteriaceae, all were sensitive to polymyxin B and meropenem. Conclusion:Antibiotics should be prescribed based on the antibiogram of individual intensive care units that can decrease antibiotic resistance. Polymyxin B and meropenem can be prescribed for gram-negative bacilli and vancomycin for Staphylococcus aureus.

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High Prevalence of Antimicrobial Resistance Among Gram-Negative Isolated Bacilli in Intensive Care Units at a Tertiary-Care Hospital in Yucatán Mexico

Medicina ◽

10.3390/medicina55090588 ◽

2019 ◽

Vol 55 (9) ◽

pp. 588 ◽

Cited By ~ 2

Author(s):

Andrés H. Uc-Cachón ◽

Carlos Gracida-Osorno ◽

Iván G. Luna-Chi ◽

Jonathan G. Jiménez-Guillermo ◽

Gloria M. Molina-Salinas

Keyword(s):

Escherichia Coli ◽

Intensive Care ◽

Antimicrobial Resistance ◽

Intensive Care Units ◽

High Resistance ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Gram Negative ◽

Resistance Rates

Background and Objectives: Antimicrobial resistance (AMR) is increasing worldwide and imposes significant life-threatening risks to several different populations, especially to those in intensive care units (ICU). The most commonly isolated organisms in ICU comprise gram-negative bacilli (GNB), and these represent a leading cause of serious infections. This study was conducted to describe the prevalence of resistance in GNB isolated from patients in adults, pediatric, and neonatal ICU in a tertiary-care hospital in Mérida, Mexico. Materials and Methods: A retrospective study was done on samples collected in Neonatal (NICU), Pediatric (PICU) and Adult (AICU) ICU of Unidad Médica de Alta Especialidad, Instituto Mexicano del Seguro Social in Mérida, México. The identification of isolates and antimicrobial susceptibility testing were performed using an automated system. Results: A total of 517 GNB strains were isolated. The most common positive culture was bronchial secretions. Pseudomonas aeruginosa was the prevalent pathogen in NICU and PICU, whereas Escherichia coli was common in the AICU. Overall, GNB exhibited a high resistance rates for Ampicillin (95.85%), Cefuroxime (84.17%), Piperacillin (82.93%), Cefotaxime (78.07%), Ceftriaxone (77.41%), Aztreonam (75.23%), Cefazolin (75.00%), and Ceftazidime (73.19%). There are significant differences in the resistance rates of GNB from different ICUs for penicillins, cephalosporins, carbapenems and fluoroquinolones drugs. Escherichia coli (multidrug-resistant [MDR] = 91.57%, highly resistant microorganisms [HRMO] = 90.36%) and Acinetobacter baumannii (MDR = 86.79%, HRMO = 83.02%) exhibited the highest percentage of MDR and HRMO profiles. The prevalence of the extended-spectrum beta-lactamases (ESBL)-producing isolates was 83.13% in E. coli, 78.84% in Klebsiella pneumoniae, and 66.67% in Proteus mirabilis, respectively. Conclusions: The high resistance rates to drugs were exhibited by our GNB isolates. Continuous surveillance and control of the use of antimicrobials are urgently needed to reduce the emergence and spreading of MDR, HRMO, and/or ESBL-producing bacilli.

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