scholarly journals VIGNA UNGUICULATA (L).WALP BY AZOTOBACTER SP. INDOLE-3-ACETIC ACID PRODUCTION AND ENHANCED PLANT GROWTH PROMOTION OF

2016 ◽  
Vol 4 (5) ◽  
pp. 1445-1450
Author(s):  
B. Sivasankari ◽  
◽  
Anandharaj. M. ◽  
F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 112 ◽  
Author(s):  
Arun Karnwal ◽  
Aradhana Dohroo

Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) endophytes resulted in better PGP activity against the other 54 isolates. Both endophytes were tested to screen indole-3-acetic acid production ability in pure culture conditions with L-tryptophan at 0, 50, 100, 200 and 500µg/ml concentrations. Results: P. fluorescens RE1 was recorded efficient for indole production in comparison to P. fluorescens RE17 at various L-tryptophan concentrations. P. fluorescens RE1 was shown to produce between 0.8 µg/ml and 11.5µg/ml of indole at various tryptophan concentrations, while RE17 produced between 1.2µg/ml and 10.2µg/ml. At 200 and 500µg/ml tryptophan concentration, P. fluorescens RE17 produced 7.4pmol/ml and 9.3pmol/ml IAA, respectively.  Conclusions: Inoculation of maize seed with P. fluorescens RE1 and RE17 showed a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes proved that plant growth regulators produced by Pseudomonas species could also play a critical role in plant growth promotion.


2017 ◽  
Vol 168 (3) ◽  
pp. 283-292 ◽  
Author(s):  
Eddie Luidy Imada ◽  
Amanda Alves de Paiva Rolla dos Santos ◽  
André Luiz Martinez de Oliveira ◽  
Mariangela Hungria ◽  
Elisete Pains Rodrigues

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