scholarly journals IN VITRO PROPAGATION OF SPATHOGLOTTIS PLICATA BLUME VIA ASYMBIOTIC SEED GERMINATION.

2017 ◽  
Vol 5 (3) ◽  
pp. 431-438
Author(s):  
Aswathi a ◽  
◽  
Sahaya Shibu ◽  
Agila Gopinath ◽  
Akhila Mohan. ◽  
...  
Keyword(s):  
Seed Germination ◽  
In Vitro Propagation ◽  
Asymbiotic Seed Germination

Efficient protocol for in vitro propagation of Laelia rubescens Lindl. from asymbiotic seed germination

2020 ◽  
Vol 133 ◽  
pp. 264-272
Author(s):  
Alberto Mayo-Mosqueda ◽  
Luis F. Maceda-López ◽  
Silvia B. Andrade-Canto ◽  
Eliana Noguera-Savelli ◽  
Humberto Caamal-Velázquez ◽  
...  
Keyword(s):  
Seed Germination ◽  
In Vitro Propagation ◽  
Asymbiotic Seed Germination

scholarly journals Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

2017 ◽  
Vol 66 (4) ◽  
pp. 544-548 ◽  
Author(s):  
Seir Antonio Salazar Mercado ◽  
Nelson Alfonso Vega Contreras
Keyword(s):  
Seed Germination ◽  
Threatened Species ◽  
Growth And Development ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Ms Medium ◽  
Organic Additives ◽  
Natural Habitats ◽  
Asymbiotic Seed Germination

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

scholarly journals Asymbiotic seed germination and in vitro propagation of Brasiliorchis picta

2016 ◽  
Vol 15 (6) ◽  
pp. 134-144 ◽  
Author(s):  
Almeida dos Santos Silvia ◽  
de Camargo Smidt Eric ◽  
Andrian Padial Andr eacute ◽  
Lopes Fortes Ribas Luciana
Keyword(s):  
Seed Germination ◽  
In Vitro Propagation ◽  
Asymbiotic Seed Germination

scholarly journals In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid

2022 ◽  
Vol 31 (2) ◽  
pp. 153-160
Author(s):  
Saranjeet Kaur
Keyword(s):  
Seed Germination ◽  
Plant Tissue ◽  
Activated Charcoal ◽  
In Vitro Propagation ◽  
In Vitro Conservation ◽  
Coconut Water ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Medicinal Orchid

The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through in vitro asymbiotic seed germination technique in Mitra orchid medium supplemented with cytokinins (Kn - 4.65 μM, BAP - 4.44 μM), and auxin (NAA- 5.37 μM). The germination frequency and initiation of germination was higher in NAA augmented medium and seedlings developed in 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication of protocorm like bodies. Activated charcoal successfully checked the release of brownish exudates in the cultures. Plant Tissue Cult. & Biotech. 31(2): 153-160, 2021 (December)

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

scholarly journals In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Growth Regulators ◽  
Subsequent Development ◽  
Ms Medium ◽  
Asymbiotic Seed Germination ◽  
Endangered Orchid ◽  
Maximum Root ◽  
Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   

In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

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