scholarly journals Campylobacter colonization is not associated with proventricular dilatation disease in psittacines

2017 ◽  
Vol Volume 8 ◽  
pp. 37-40
Author(s):  
Holden Bulbow ◽  
Jing Wu ◽  
Debra Turner ◽  
Michael McEntire ◽  
Ian Tizard
Keyword(s):  
Proventricular Dilatation Disease

scholarly journals A New Multiplex Real-Time RT-PCR for Simultaneous Detection and Differentiation of Avian Bornaviruses

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1358
Author(s):  
Brigitte Sigrist ◽  
Jessica Geers ◽  
Sarah Albini ◽  
Dennis Rubbenstroth ◽  
Nina Wolfrum
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Epidemiological Studies ◽  
Virus Species ◽  
Rt Pcr ◽  
P Gene ◽  
Field Samples ◽  
Causative Agents ◽  
Species Specific ◽  
Proventricular Dilatation Disease

Avian bornaviruses were first described in 2008 as the causative agents of proventricular dilatation disease (PDD) in parrots and their relatives (Psittaciformes). To date, 15 genetically highly diverse avian bornaviruses covering at least five viral species have been discovered in different bird orders. Currently, the primary diagnostic tool is the detection of viral RNA by conventional or real-time RT-PCR (rRT-PCR). One of the drawbacks of this is the usage of either specific assays, allowing the detection of one particular virus, or of assays with a broad detection spectrum, which, however, do not allow for the simultaneous specification of the detected virus. To facilitate the simultaneous detection and specification of avian bornaviruses, a multiplex real-time RT-PCR assay was developed. Whole-genome sequences of various bornaviruses were aligned. Primers were designed to recognize conserved regions within the overlapping X/P gene and probes were selected to detect virus species-specific regions within the target region. The optimization of the assay resulted in the sensitive and specific detection of bornaviruses of Psittaciformes, Passeriformes, and aquatic birds. Finally, the new rRT-PCR was successfully employed to detect avian bornaviruses in field samples from various avian species. This assay will serve as powerful tool in epidemiological studies and will improve avian bornavirus detection.

scholarly journals Recombinant Modified Vaccinia Virus Ankara (MVA) Vaccines Efficiently Protect Cockatiels Against Parrot Bornavirus Infection and Proventricular Dilatation Disease

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1130 ◽  
Author(s):  
Isabell Rall ◽  
Ralf Amann ◽  
Sara Malberg ◽  
Christiane Herden ◽  
Dennis Rubbenstroth
Keyword(s):  
Vaccinia Virus ◽  
Challenge Infection ◽  
Control Group ◽  
Viral Loads ◽  
Breeding Populations ◽  
Modified Vaccinia Virus Ankara ◽  
Associated Lesions ◽  
Unvaccinated Control ◽  
Causative Agents ◽  
Proventricular Dilatation Disease

Parrot bornaviruses (PaBVs) are the causative agents of proventricular dilatation disease (PDD), a chronic and often fatal neurologic disorder in Psittaciformes. The disease is widely distributed in private parrot collections and threatens breeding populations of endangered species. Thus, immunoprophylaxis strategies are urgently needed. In previous studies we demonstrated a prime-boost vaccination regime using modified vaccinia virus Ankara (MVA) and Newcastle disease virus (NDV) constructs expressing the nucleoprotein and phosphoprotein of PaBV-4 (MVA/PaBV-4 and NDV/PaBV-4, respectively) to protect cockatiels (Nymphicus hollandicus) against experimental challenge infection. Here we investigated the protective effect provided by repeated immunization with either MVA/PaBV-4, NDV/PaBV-4 or Orf virus constructs (ORFV/PaBV-4) individually. While MVA/PaBV-4-vaccinated cockatiels were completely protected against subsequent PaBV-2 challenge infection and PDD-associated lesions, the course of the challenge infection in NDV/PaBV-4- or ORFV/PaBV-4-vaccinated birds did not differ from the unvaccinated control group. We further investigated the effect of vaccination on persistently PaBV-4-infected cockatiels. Remarkably, subsequent immunization with MVA/PaBV-4 and NDV/PaBV-4 neither induced obvious immunopathogenesis exacerbating the disease nor reduced viral loads in the infected birds. In summary, we demonstrated that vaccination with MVA/PaBV-4 alone is sufficient to efficiently prevent PaBV-2 challenge infection in cockatiels, providing a suitable vaccine candidate against avian bornavirus infection and bornavirus-induced PDD.

scholarly journals Proventricular Dilatation Disease Associated with Avian Bornavirus Infection in a Citron-Crested Cockatoo that Was Born and Hand-Reared in Japan

2011 ◽  
Vol 73 (6) ◽  
pp. 837-840 ◽  
Author(s):  
Hirohito OGAWA ◽  
Yasuyuki SANADA ◽  
Naoko SANADA ◽  
Megumi KUDO ◽  
Kotaro TUCHIYA ◽  
...  
Keyword(s):  
Avian Bornavirus ◽  
Proventricular Dilatation Disease

scholarly journals Novel Avian Bornavirus in a Nonpsittacine Species (Canary; Serinus canaria) with Enteric Ganglioneuritis and Encephalitis

2009 ◽  
Vol 83 (21) ◽  
pp. 11367-11371 ◽  
Author(s):  
Herbert Weissenböck ◽  
Karin Sekulin ◽  
Tamás Bakonyi ◽  
Sandra Högler ◽  
Norbert Nowotny
Keyword(s):  
Sequence Analysis ◽  
Reverse Transcription ◽  
Avian Species ◽  
Serinus Canaria ◽  
Viral Dissemination ◽  
Reverse Transcription Pcr ◽  
The Family ◽  
Avian Bornavirus ◽  
Proventricular Dilatation Disease ◽  
Unique Genotype

ABSTRACT A canary bird (Serinus canaria) died with nonsuppurative ganglioneuritis of the proventriculus and gizzard and encephalitis, lesions comparable to proventricular dilatation disease (PDD) of psittacine birds. Recently, several genotypes of a novel avian bornavirus have been linked to PDD. In the canary, bornaviral antigen was detected by immunohistochemistry in both neural and extraneural tissues. The widespread viral dissemination was confirmed by reverse transcription-PCR. Sequence analysis revealed a unique genotype of avian bornavirus. This observation suggests that bornaviruses are natural pathogens of several avian species and that the family Bornaviridae comprises more viral genotypes (or viral species) than previously assumed.

scholarly journals Analysis of Naturally Occurring Avian Bornavirus Infection and Transmission during an Outbreak of Proventricular Dilatation Disease among Captive Psittacine Birds

2009 ◽  
Vol 84 (4) ◽  
pp. 2176-2179 ◽  
Author(s):  
Amy L. Kistler ◽  
Jeanne M. Smith ◽  
Alexander L. Greninger ◽  
Joseph L. DeRisi ◽  
Don Ganem
Keyword(s):  
Western Blotting ◽  
Reverse Transcription ◽  
Fatal Case ◽  
Natural Conditions ◽  
Reverse Transcription Pcr ◽  
Naturally Occurring ◽  
Avian Bornavirus ◽  
Proventricular Dilatation Disease

ABSTRACT A proventricular dilatation disease (PDD) outbreak provided the opportunity to investigate the transmissibility of avian Bornavirus (ABV) and its linkage to PDD under natural conditions. Upon exposure to a bird with a fatal case of PDD, 10 birds became symptomatic and died. ABV2 RNA was recovered from available tissues. Further screening revealed that 12/46 exposed birds were ABV2+. Three chicks boarded at this aviary developed PDD. They harbored the same ABV2 isolate and transmitted it to five of eight chicks in their home aviary. These findings demonstrate that ABV infection precedes the development of PDD. ABV-specific Western blotting and reverse transcription-PCR indicate that ABV2 is not strictly neurotropic.

scholarly journals Avian Bornavirus Associated with Fatal Disease in Psittacine Birds

2010 ◽  
Vol 84 (13) ◽  
pp. 6269-6275 ◽  
Author(s):  
Peter Staeheli ◽  
Monika Rinder ◽  
Bernd Kaspers
Keyword(s):  
New Technologies ◽  
Rna Virus ◽  
Single Species ◽  
Farm Animals ◽  
Research Groups ◽  
Fatal Disease ◽  
Highly Pathogenic ◽  
Avian Bornavirus ◽  
Proventricular Dilatation Disease ◽  
Borna Disease

ABSTRACT Thanks to new technologies which enable rapid and unbiased screening for viral nucleic acids in clinical specimens, an impressive number of previously unknown viruses have recently been discovered. Two research groups independently identified a novel negative-strand RNA virus, now designated avian bornavirus (ABV), in parrots with proventricular dilatation disease (PDD), a severe lymphoplasmacytic ganglioneuritis of the gastrointestinal tract of psittacine birds that is frequently accompanied by encephalomyelitis. Since its discovery, ABV has been detected worldwide in many captive parrots and in one canary with PDD. ABV induced a PDD-like disease in experimentally infected cockatiels, strongly suggesting that ABV is highly pathogenic in psittacine birds. Until the discovery of ABV, the Bornaviridae family consisted of a single species, classical Borna disease virus (BDV), which is the causative agent of a progressive neurological disorder that affects primarily horses, sheep, and some other farm animals in central Europe. Although ABV and BDV share many biological features, there exist several interesting differences, which are discussed in this review.

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