scholarly journals RTS,S malaria vaccine development: progress and considerations for postapproval introduction

2016 ◽  
pp. 25 ◽  
Author(s):  
Kwaku Poku Asante ◽  
George Adjei ◽  
Yeetey Enuameh ◽  
Seth Owusu-Agyei
Keyword(s):  
Malaria Vaccine ◽  
Vaccine Development

scholarly journals In silico characterisation of putative Plasmodium falciparum vaccine candidates in African malaria populations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
O. Ajibola ◽  
M. F. Diop ◽  
A. Ghansah ◽  
L. Amenga-Etego ◽  
L. Golassa ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Vaccine ◽  
Transmembrane Domain ◽  
Vaccine Development ◽  
Balancing Selection ◽  
Immune Recognition ◽  
African Countries ◽  
Vaccine Candidates ◽  
D Values ◽  
Tajima’S D

AbstractGenetic diversity of surface exposed and stage specific Plasmodium falciparum immunogenic proteins pose a major roadblock to developing an effective malaria vaccine with broad and long-lasting immunity. We conducted a prospective genetic analysis of candidate antigens (msp1, ama1, rh5, eba175, glurp, celtos, csp, lsa3, Pfsea, trap, conserved chrom3, hyp9, hyp10, phistb, surfin8.2, and surfin14.1) for malaria vaccine development on 2375 P. falciparum sequences from 16 African countries. We described signatures of balancing selection inferred from positive values of Tajima’s D for all antigens across all populations except for glurp. This could be as a result of immune selection on these antigens as positive Tajima’s D values mapped to regions with putative immune epitopes. A less diverse phistb antigen was characterised with a transmembrane domain, glycophosphatidyl anchors between the N and C- terminals, and surface epitopes that could be targets of immune recognition. This study demonstrates the value of population genetic and immunoinformatic analysis for identifying and characterising new putative vaccine candidates towards improving strain transcending immunity, and vaccine efficacy across all endemic populations.

scholarly journals Viral vectors in malaria vaccine development

2009 ◽  
Vol 31 (9) ◽  
pp. 501-519 ◽  
Author(s):  
K. J. LIMBACH ◽  
T. L. RICHIE
Keyword(s):  
Viral Vectors ◽  
Malaria Vaccine ◽  
Vaccine Development

Malaria Vaccine Development

2010 ◽  
pp. 409-422
Author(s):  
Yimin Wu ◽  
Ruth Ellis ◽  
Kazutoyo Miura ◽  
David Narum ◽  
Louis H. Miller
Keyword(s):  
Malaria Vaccine ◽  
Vaccine Development

Preerythrocytic malaria vaccine development

2007 ◽  
Vol 20 (5) ◽  
pp. 461-466 ◽  
Author(s):  
Sebastian A Mikolajczak ◽  
Ahmed SI Aly ◽  
Stefan HI Kappe
Keyword(s):  
Malaria Vaccine ◽  
Vaccine Development

scholarly journals Cross-Species Immunity in Malaria Vaccine Development: Two, Three, or Even Four for the Price of One?

2007 ◽  
Vol 76 (3) ◽  
pp. 873-878 ◽  
Author(s):  
Bruno Douradinha ◽  
Maria M. Mota ◽  
Adrian J. F. Luty ◽  
Robert W. Sauerwein
Keyword(s):  
Malaria Vaccine ◽  
Vaccine Development

Malaria Vaccine Development Using Synthetic Peptides as a Technical Platform

2012 ◽  
pp. 107-149 ◽  
Author(s):  
Giampietro Corradin ◽  
Nora Céspedes ◽  
Antonio Verdini ◽  
Andrey V. Kajava ◽  
Myriam Arévalo-Herrera ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Malaria Vaccine ◽  
Vaccine Development ◽  
Technical Platform

scholarly journals The Plasmodium falciparum circumsporozoite protein produced in Lactococcus lactis is pure and stable

2019 ◽  
Vol 295 (2) ◽  
pp. 403-414 ◽  
Author(s):  
Susheel K. Singh ◽  
Jordan Plieskatt ◽  
Bishwanath Kumar Chourasia ◽  
Vandana Singh ◽  
Judith M. Bolscher ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Lactococcus Lactis ◽  
Malaria Vaccine ◽  
Vaccine Development ◽  
Expression System ◽  
Surface Protein ◽  
Circumsporozoite Protein ◽  
Full Length

The Plasmodium falciparum circumsporozoite protein (PfCSP) is a sporozoite surface protein whose role in sporozoite motility and cell invasion has made it the leading candidate for a pre-erythrocytic malaria vaccine. However, production of high yields of soluble recombinant PfCSP, including its extensive NANP and NVDP repeats, has proven problematic. Here, we report on the development and characterization of a secreted, soluble, and stable full-length PfCSP (containing 4 NVDP and 38 NANP repeats) produced in the Lactococcus lactis expression system. The recombinant full-length PfCSP, denoted PfCSP4/38, was produced initially with a histidine tag and purified by a simple two-step procedure. Importantly, the recombinant PfCSP4/38 retained a conformational epitope for antibodies as confirmed by both in vivo and in vitro characterizations. We characterized this complex protein by HPLC, light scattering, MS analysis, differential scanning fluorimetry, CD, SDS-PAGE, and immunoblotting with conformation-dependent and -independent mAbs, which confirmed it to be both pure and soluble. Moreover, we found that the recombinant protein is stable at both frozen and elevated-temperature storage conditions. When we used L. lactis–derived PfCSP4/38 to immunize mice, it elicited high levels of functional antibodies that had the capacity to modify sporozoite motility in vitro. We concluded that the reported yield, purity, results of biophysical analyses, and stability of PfCSP4/38 warrant further consideration of using the L. lactis system for the production of circumsporozoite proteins for preclinical and clinical applications in malaria vaccine development.

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