scholarly journals Characterization of Sonic Hedgehog/Gli1 Signal Expression in Human Ureter Either Un-Stented or Fitted with Double-Pigtail Stent or a Thread

2021 ◽  
Vol Volume 13 ◽  
pp. 529-533
Author(s):  
Benoît Vogt ◽  
Ilham Chokri
Keyword(s):  
Sonic Hedgehog ◽  
Pigtail Stent ◽  
Human Ureter

Characterization of sonic hedgehog as a novel NF‐κB target gene that promotes NF‐κB‐mediated apoptosis resistance and tumor growth in vivo

2008 ◽  
Vol 23 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Hubert Kasperczyk ◽  
Bernd Baumann ◽  
Klaus‐Michael Debatin ◽  
Simone FuMa
Keyword(s):  
Tumor Growth ◽  
Sonic Hedgehog ◽  
Target Gene ◽  
Apoptosis Resistance

scholarly journals Histological Inflammation in Human Ureter either Healthy or Fitted with Double-Pigtail Stent or a Thin 0.3 F Suture Thread: A Preliminary Study

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Benoît Vogt ◽  
Ilham Chokri
Keyword(s):  
Histological Section ◽  
Muscle Layer ◽  
Number Of Patients ◽  
Suture Thread ◽  
Preliminary Study ◽  
Pigtail Stent ◽  
Hematoxylin Eosin ◽  
Human Ureter ◽  
Inflammation Score

Background. Ureteral stent intolerance reduces patients’ quality of life. It has been suggested that changes in the shape of stents could decrease discomfort. In previous studies, the innovative pigtail-suture stent (i.e., JFil® or MiniJFil®) with a thin 0.3 F suture thread significantly decreased stent-related symptoms. Fortuitously, a dilation of the ureter containing the sutures was discovered. In addition, no inflammation was seen on the ureter wall around the suture in endoscopy. In this preliminary study, we assessed ureteral inflammation in the human ureter when it was healthy or when fitted with a double-pigtail stent or a thread. Materials and Methods. After consent and inclusion of patients in the protocol, fifteen segments of ureters were collected during cystectomy procedures for bladder tumors. Ureteral inflammation was assessed on the histological section stained with hematoxylin-eosin. Histological grading (cumulative range of 0 to 6) assessing inflammation was performed on the ureter section for mucosa inflammation and inflammation in the muscle layer. Results. A marked ureteral inflammatory reaction was observed in all cases of ureters fitted with a double-pigtail stent with a mean inflammation score of 4.8 ± 0.4. The ureter fitted with the thin suture thread showed inflammation in only one case with a mean inflammation score of 1.8 ± 1.3 p=0.001. Conclusion. Although the study was limited by the small number of patients, it confirmed that the double-pigtail stent induced ureteral inflammation in all cases and the thin 0.3 F suture thread caused less ureteral inflammation than the double-pigtail stent. The concept of material reduction within the urinary tract seems necessary in order to decrease mucosal irritation. The JFil® or the MiniJFil® thread could meet this requirement.

Complex expression of the zp-50 pou gene in the embryonic zebrafish brain is altered by overexpression of sonic hedgehog

Development ◽  
1996 ◽  
Vol 122 (6) ◽  
pp. 1769-1780 ◽  
Author(s):  
G. Hauptmann ◽  
T. Gerster
Keyword(s):  
Sonic Hedgehog ◽  
Similar Response ◽  
Intercellular Signaling ◽  
Class Iii ◽  
Dorsoventral Patterning ◽  
Gene Encoding ◽  
Pou Domain ◽  
Distinct Cell ◽  
The Central Nervous System

We report the characterization of the zebrafish zp-50 class III POU domain gene. This gene is first activated in the prospective diencephalon after the end of the gastrula period. During somitogenesis, zp-50 is expressed in a very dynamic and complex fashion in all major subdivisions of the central nervous system. After one day of development, zp-50 transcripts are present in the fore- and midbrain in several distinct cell clusters. In the hindbrain, zp-50 expression is found in two types of domains. Correct zp-50 expression in the ventral fore- and midbrain requires genes known to be involved in dorsoventral patterning of the zebrafish CNS. Transcripts of the sonic hedgehog (shh) gene encoding an intercellular signaling molecule are detected in the forming diencephalon shortly prior to the appearance of zp-50 mRNA. Correct expression in this region of both shh, and zp-50, requires a functional cyclops (cyc) locus: shh and zp-50 transcripts are likewise absent from the ventral rostral brain of mutant cyc−/− embryos. Injection of synthetic shh mRNA into fertilized eggs causes ectopic zp-50 expression at more dorsal positions of the embryonic brain. The close spatial and temporal coincidence of expression in the rostral brain, the similar response to the cyc- mutation, and the ectopic zp-50 expression in the injection experiments all suggest that zp-50 may directly respond to the reception of the Shh signal.

scholarly journals Genomic characterization of Gli-activator targets in sonic hedgehog-mediated neural patterning

Development ◽  
2007 ◽  
Vol 134 (10) ◽  
pp. 1977-1989 ◽  
Author(s):  
S. A. Vokes ◽  
H. Ji ◽  
S. McCuine ◽  
T. Tenzen ◽  
S. Giles ◽  
...  
Keyword(s):  
Sonic Hedgehog ◽  
Neural Patterning ◽  
Genomic Characterization

Characterization of the promoter region and genomic organization of GLI; a member of the Sonic hedgehog-Patched signaling pathway

Gene ◽  
1998 ◽  
Vol 209 (1-2) ◽  
pp. 1-11 ◽  
Author(s):  
Cheng Zheng Liu ◽  
Jian Tao Yang ◽  
Joon Won Yoon ◽  
David Walterhouse ◽  
Philip Iannaccone
Keyword(s):  
Signaling Pathway ◽  
Sonic Hedgehog ◽  
Promoter Region ◽  
Genomic Organization

scholarly journals The structure of the oligosaccharides of alpha3beta1 integrin from human ureter epithelium (HCV29) cell line.

2002 ◽  
Vol 49 (2) ◽  
pp. 491-500 ◽  
Author(s):  
Anna Lityńska ◽  
Ewa Pocheć ◽  
Dorota Hoja-Lukowicz ◽  
Elzbieta Kremser ◽  
Piotr Laidler ◽  
...  
Keyword(s):  
Cell Line ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Beta Subunits ◽  
Ionization Mass ◽  
Complex Type ◽  
Peptide Characterization ◽  
Alpha3beta1 Integrin ◽  
Human Ureter

There is a growing line of evidence that glycosylation of alpha and beta subunits is important for the function of integrins. Integrin alpha3beta1, from human ureter epithelium cell-line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin alpha3beta1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.

scholarly journals 1080 – Characterization of Sonic Hedgehog in Esophageal Cancerderived Exosomes

2019 ◽  
Vol 156 (6) ◽  
pp. S-228
Author(s):  
Wei Zhang ◽  
Thai Pham ◽  
Qiuyang Zhang ◽  
David H. Wang
Keyword(s):  
Sonic Hedgehog
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