scholarly journals Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model

2018 ◽  
Vol Volume 10 ◽  
pp. 127-133
Author(s):  
Safendra Siregar ◽  
Reni Farenia ◽  
Suwandi Sugandi ◽  
Rully M Roesli
Keyword(s):  
New Zealand ◽  
Angiotensin Ii ◽  
Urethral Stricture ◽  
Collagen Type ◽  
Collagen Type I ◽  
Angiotensin Ii Receptor Blocker ◽  
Type I ◽  
Tgf Beta ◽  
Angiotensin Ii Receptor ◽  
Zealand Rabbit

PPARβ/δ activation inhibits angiotensin II-induced collagen type I expression in rat cardiac fibroblasts

2007 ◽  
Vol 460 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Huijie Zhang ◽  
Rongbiao Pi ◽  
Ruifang Li ◽  
Ping Wang ◽  
Futian Tang ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Collagen Type ◽  
Cardiac Fibroblasts ◽  
Collagen Type I ◽  
Type I

scholarly journals Discoidin Domain Receptor 2 regulates AT1 receptor expression in Angiotensin II-stimulated cardiac fibroblasts via fibronectin-dependent Integrin-β1 signalling

2021 ◽  
Author(s):  
Allen Sam Titus ◽  
Harikrishnan V ◽  
Mingyi Wang ◽  
Edward G Lakkatta ◽  
Shivakumar Kailasam
Keyword(s):  
Angiotensin Ii ◽  
Collagen Type ◽  
Cardiac Fibroblasts ◽  
Collagen Type I ◽  
Cardiac Fibroblast ◽  
Regulatory Role ◽  
Type I ◽  
Integrin Β1 ◽  
Fibronectin Expression

Fibronectin is an extracellular matrix glycoprotein with a regulatory role in fundamental cellular processes. Recent reports on the cardioprotective effect of fibronectin inhibition in a setting of myocardial injury suggest a role for fibronectin in cardiac fibroblast function, which remains largely unexplored. This study probed the molecular basis and functional implications of fibronectin gene expression in cardiac fibroblasts exposed to Angiotensin II, a potent pro-fibrotic factor in the myocardium. Using gene knockdown and over-expression approaches, western blotting and promoter pull-down assay, we show that collagen type I-activated Discoidin Domain Receptor 2 (DDR2) mediates Angiotensin II-stimulated transcriptional up-regulation of fibronectin expression by Yes-activated Protein in cardiac fibroblasts. Further, siRNA-mediated fibronectin knockdown attenuated Angiotensin II-dependent expression of anti-apoptotic cIAP2 and promoted cell death under oxidative stress. Fibronectin was also found to mediate Angiotensin II-stimulated collagen type I expression. Importantly, an obligate role for fibronectin was observed in Angiotensin II-stimulated expression of its receptor, AT1R, which would link ECM signalling and Angiotensin II signalling in cardiac fibroblasts. Moreover, the regulatory role of DDR2-dependent fibronectin expression in Ang II-stimulated cIAP2, collagen type I and AT1R expression was mediated by Integrin-β1-integrin-linked kinase signalling. The pro-survival role of fibronectin in cardiac fibroblasts and its regulatory role in collagen and AT1R expression, downstream of DDR2, could be critical determinants of cardiac fibroblast-mediated wound healing following myocardial injury. Our findings point to a complex mechanism of regulation of cardiac fibroblast function involving two major extracellular matrix proteins, collagen type I and fibronectin, and their receptors, DDR2 and Integrin-β1.

scholarly journals Angiotensin II Activates Collagen Type I Gene in the Renal Cortex and Aorta of Transgenic Mice through Interaction with Endothelin and TGF-β

2001 ◽  
Vol 12 (12) ◽  
pp. 2701-2710
Author(s):  
Fadi Fakhouri ◽  
Sandrine Placier ◽  
Raymond Ardaillou ◽  
Jean-Claude Dussaule ◽  
Christos Chatziantoniou
Keyword(s):  
Angiotensin Ii ◽  
Transgenic Mice ◽  
Collagen Type ◽  
Renal Cortex ◽  
Collagen Type I ◽  
Collagen I ◽  
Type I ◽  
Cortical Slices ◽  
I Gene

ABSTRACT. Hypertension is frequently associated with the development of renal vascular fibrosis. This pathophysiologic process is due to the abnormal formation of extracellular matrix proteins, mainly collagen type I. In previous studies, it has been observed that the pharmacologic blockade of angiotensin II (Ang II) or endothelin (ET) blunted the development of glomerulo- and nephroangiosclerosis in nitric oxide-deficient hypertensive animals by inhibiting collagen I gene activation. The purpose of this study was to investigate whether and how AngII interacts with ET to activate the collagen I gene and whether transforming growth factor-β (TGF-β) could be a player in this interaction. Experiments were performedin vivoon transgenic mice harboring the luciferase gene under the control of the collagen I-α2 chain promoter (procolα2[I]). Bolus intravenous administration of AngII or ET produced a rapid, dose-dependent activation of collagen I gene in aorta and renal cortical slices (threefold increase over control at 2 h,P< 0.01). The AngII-induced effect on procolα2(I) was completely inhibited by candesartan (AngII type 1 receptor antagonist) and substantially blunted by bosentan (dual ET receptor antagonist) (P< 0.01), whereas the ET-induced activation of collagen I gene was blocked only by bosentan. In subsequent experiments, TGF-β (also administered intravenously) produced a rapid increase of procolα2(I) in aorta and renal cortical slices (twofold increase over control at 1 h,P< 0.01) that was completely blocked by decorin (scavenger of the active form of TGF-β). In addition, decorin attenuated the activation of collagen I gene produced by AngII (P< 0.01). These data indicate that AngII can activate collagen I gene in aorta and renal cortexin vivoby a mechanism(s) requiring participation and/or cooperation of ET and TGF-β.

scholarly journals Angiotensin II Regulation of Collagen Type I Expression in Cardiac Fibroblasts

Hypertension ◽  
2004 ◽  
Vol 44 (5) ◽  
pp. 655-661 ◽  
Author(s):  
Kui Chen ◽  
Jiawei Chen ◽  
Dayuan Li ◽  
Xingjian Zhang ◽  
Jawahar L. Mehta
Keyword(s):  
Angiotensin Ii ◽  
Collagen Type ◽  
Cardiac Fibroblasts ◽  
Collagen Type I ◽  
Type I

scholarly journals Culture on a Fragmin/protamine-Coated Plate Suppresses the Collagen Type I^|^alpha;I and TGF-^|^beta;1 mRNA Expression of Rat Hepatic Stellate RI-T Cells

2013 ◽  
Vol 75 (5) ◽  
pp. 553-559 ◽  
Author(s):  
Hitomi SEKIGUCHI ◽  
Natsuko HEMMI ◽  
Takehiro MAKI ◽  
Aisa OZAWA ◽  
Erina KADOWAKI ◽  
...  
Keyword(s):  
T Cells ◽  
Mrna Expression ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Tgf Beta ◽  
Coated Plate

scholarly journals Substance P increases CCN2 dependent on TGF-beta yet Collagen Type I via TGF-beta1 dependent and independent pathways in tenocytes

2017 ◽  
Vol 59 (1) ◽  
pp. 30-44 ◽  
Author(s):  
Nagat Frara ◽  
Paul W. Fisher ◽  
Yingjie Zhao ◽  
Joseph T. Tarr ◽  
Mamta Amin ◽  
...  
Keyword(s):  
Substance P ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Tgf Beta
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