scholarly journals Antioxidant activity of capsaicin on radiation-induced oxidation of murine hepatic mitochondrial membrane preparation

2015 ◽  
pp. 163 ◽  
Author(s):  
Ravi Joshi ◽  
R Gangabhagirathi
Keyword(s):  
Antioxidant Activity ◽  
Mitochondrial Membrane ◽  
Membrane Preparation ◽  
Radiation Induced

Antioxidant activity in sugarcane juice and its protective role against radiation induced DNA damage

2008 ◽  
Vol 106 (3) ◽  
pp. 1154-1160 ◽  
Author(s):  
U.S. Kadam ◽  
S.B. Ghosh ◽  
Strayo De ◽  
P. Suprasanna ◽  
T.P.A. Devasagayam ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Activity ◽  
Protective Role ◽  
Sugarcane Juice ◽  
Radiation Induced

scholarly journals Evaluation of the Antioxidant Activity of Nigella sativa L. and Allium ursinum Extracts in a Cellular Model of Doxorubicin-Induced Cardiotoxicity

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5259
Author(s):  
Raluca Maria Pop ◽  
Ioana Corina Bocsan ◽  
Anca Dana Buzoianu ◽  
Veronica Sanda Chedea ◽  
Sonia Ancuța Socaci ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Mitochondrial Membrane ◽  
Methanolic Extract ◽  
Nigella Sativa ◽  
Membrane Depolarization ◽  
Cellular Model ◽  
H9c2 Cells ◽  
Mitochondrial Membrane Depolarization ◽  
Ft Ir

Natural products black cumin—Nigella sativa (N. sativa) and wild garlic—Allium ursinum (AU) are known for their potential role in reducing cardiovascular risk factors, including antracycline chemotherapy. Therefore, this study investigates the effect of N. sativa and AU water and methanolic extracts in a cellular model of doxorubicin (doxo)-induced cardiotoxicity. The extracts were characterized using Ultraviolet-visible (UV-VIS) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, Liquid Chromatography coupled with Mass Spectrometry (LC-MS) and Gas Chromatography coupled with Mass Spectrometry (GC-MS) techniques. Antioxidant activity was evaluated on H9c2 cells. Cytosolic and mitochondrial reactive oxygen species (ROS) release was evaluated using 2′,7′-dichlorofluorescin-diacetate (DHCF-DA) and mitochondria-targeted superoxide indicator (MitoSOX red), respectively. Mitochondrial membrane depolarization was evaluated by flow cytometry. LC-MS analysis identified 12 and 10 phenolic compounds in NSS and AU extracts, respectively, with flavonols as predominant compounds. FT-IR analysis identified the presence of carbohydrates, amino acids and lipids in both plants. GC-MS identified the sulfur compounds in the AU water extract. N. sativa seeds (NSS) methanolic extract had the highest antioxidant activity reducing both intracellular and mitochondrial ROS release. All extracts (excepting AU methanolic extract) preserved H9c2 cells viability. None of the investigated plants affected the mitochondrial membrane depolarization. N. sativa and AU are important sources of bioactive compounds with increased antioxidant activities, requiring different extraction solvents to obtain the pharmacological effects.

scholarly journals Sodium Tanshinone IIA Sulfonate Prevents Radiation-Induced Toxicity in H9c2 Cardiomyocytes

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Wenjing Zhang ◽  
Yi Li ◽  
Rui Li ◽  
Yaya Wang ◽  
Mengwen Zhu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Antioxidant Activity ◽  
Cell Cycle Arrest ◽  
Tanshinone Iia ◽  
H9c2 Cells ◽  
X Ray ◽  
Cycle Arrest ◽  
H9c2 Cardiomyocytes ◽  
Radiation Induced

The present study was designed to elucidate the key parameters associated with X-ray radiation induced oxidative stress and the effects of STS on X-ray-induced toxicity in H9c2 cardiomyocytes. Cytotoxicity of STS and radiation was assessed by MTT. Antioxidant activity was evaluated by SOD and MDA. Apoptosis was measured by the flow cytometry, Hoechst 33258, clonogenic survival assay, and western blot. It was found that the cell viability of H9c2 cells exposed to X-ray radiation was significantly decreased in a dose-dependent manner and was associated with cell cycle arrest at the G0/G1 phase as well as apoptosis. STS treatment significantly reversed the morphological changes, attenuated radiation-induced apoptosis, and improved the antioxidant activity in the H9c2 cells. STS significantly increased the Bcl-2 and Bcl-2/Bax levels and decreased the Bax and caspase-3 levels, compared with the cells treated with radiation alone. STS treatment also resulted in a significant increase in p38-MAPK activation. STS could protect the cells from X-ray-induced cell cycle arrest, oxidative stress, and apoptosis. Therefore, we suggest the STS could be useful for the treatment of radiation-induced cardiovascular injury.

Antioxidant Power, Lipid Oxidation, Color, and Viability of Listeria monocytogenes in Beef Bologna Treated with Gamma Radiation and Containing Various Levels of Glucose†

2002 ◽  
Vol 65 (11) ◽  
pp. 1750-1755 ◽  
Author(s):  
CHRISTOPHER H. SOMMERS ◽  
XUETONG FAN
Keyword(s):  
Antioxidant Activity ◽  
Listeria Monocytogenes ◽  
Ionizing Radiation ◽  
Lipid Oxidation ◽  
Thermal Processing ◽  
Meat Products ◽  
Color Analysis ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Radiation Induced

Ionizing radiation can be used to pasteurize ready-to-eat (RTE) meat products. Thermal processing of RTE meats that contain dextrose results in the production of antioxidants that may interfere with ionizing radiation pasteurization of RTE meat products. Beef bologna was manufactured with dextrose concentrations of 0, 2, 4, 6, and 8%. Antioxidant activity, as measured by the Ferric Reducing Antioxidant Power assay, increased with dextrose concentration but was unaffected by ionizing radiation. Lipid oxidation increased significantly in irradiated bologna (4 kGy) that contained dextrose. Hunter color analysis indicated that the addition of dextrose reduced the ionizing radiation-induced loss of redness (a-value) but promoted the loss of brightness (L-value). The radiation resistance, D10-value, of Listeria monocytogenes that was surface-inoculated onto bologna slices was not affected by dextrose concentration. L. monocytogenes strains isolated from RTE meats after listeriosis outbreaks were utilized. Increased antioxidant activity generated by thermal processing of dextrose in fine emulsion sausages does not present a barrier to radiation pasteurization of RTE meats. However, a high dextrose concentration in combination with gamma irradiation increases lipid oxidation significantly.

scholarly journals A Multiparametric Study of Internalization of Fullerenol C60(OH)36 Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation

2020 ◽  
Vol 21 (7) ◽  
pp. 2281 ◽  
Author(s):  
Anna Lichota ◽  
Ireneusz Piwoński ◽  
Sylwia Michlewska ◽  
Anita Krokosz
Keyword(s):  
Oxidative Stress ◽  
Ionizing Radiation ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mitochondrial Membrane ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Radiation Induced ◽  
Radioprotective Properties

The aim of this study was to investigate the uptake and accumulation of fullerenol C60(OH)36 into peripheral blood mononuclear cells (PBMCs). Some additional studies were also performed: measurement of fullerenol nanoparticle size, zeta potential, and the influence of fullerenol on the ionizing radiation-induced damage to PMBCs. Fullerenol C60(OH)36 demonstrated an ability to accumulate in PBMCs. The accumulation of fullerenol in those cells did not have a significant effect on cell survival, nor on the distribution of phosphatidylserine in the plasma membrane. However, fullerenol-induced depolarization of the mitochondrial membrane proportional to the compound level in the medium was observed. Results also indicated that increased fullerenol level in the medium was associated with its enhanced transport into cells, corresponding to its influence on the mitochondrial membrane. The obtained results clearly showed the ability of C60(OH)36 to enter cells and its effect on PBMC mitochondrial membrane potential. However, we did not observe radioprotective properties of fullerenol under the conditions used in our study.

scholarly journals Inhibitory Effect of Ursolic Acid on Ultraviolet B Radiation-Induced Oxidative Stress and Proinflammatory Response-Mediated Senescence in Human Skin Dermal Fibroblasts

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Ramachandran Samivel ◽  
Rajendra Prasad Nagarajan ◽  
Umadevi Subramanian ◽  
Adnan Ali Khan ◽  
Ali Masmali ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Human Skin ◽  
Mitochondrial Membrane ◽  
Antioxidant Status ◽  
Dermal Fibroblasts ◽  
Ultraviolet B ◽  
Uvb Radiation ◽  
Proinflammatory Response ◽  
Radiation Induced ◽  
Apoptotic Induction

Ultraviolet radiation is an environmental carcinogenic agent that enhances inflammation and immunological reactions in the exposed human skin cells leading to oxidative photoaging of the epidermal and dermal segment. In the present study, we investigated the protective role of ursolic acid (UA) against ultraviolet B (UVB) radiation- induced photoaging an in vitro model of human skin dermal fibroblasts. UA-pretreated human skin dermal fibroblast (HDF) cells were exposed to UVB radiation to evaluated cell viability, reactive oxygen species (ROS), mitochondrial membrane potential, lipid peroxidation, antioxidant status, DNA damage, proinflammatory response, apoptotic induction, and matrix metalloproteinase (MMP) alteration. The UA pretreatment of HDFs mitigated the UVB irradiation-induced cytotoxicity, ROS generation, and mitochondrial membrane potential alteration and lipid peroxidation, depletion of antioxidant status, DNA damage, and apoptotic induction. UA pretreatment of HDFs also attenuated the UVB-induced expression of inflammatory (TNF-α and NF-κB) and apoptotic (p53, Bax, and caspase-3) and MMPs (MMP-2 and MMP-9) and enhanced the Bcl-2 protein levels in 20 μM UA treatment, when compared to concentrations. Hence, these results revealed that UA has the potential to mitigate UVB-induced extracellular damage by interfering with the ROS-mediated apoptotic induction and photoaging senescence and thus is a potential therapeutic agent to protect the skin against UVB-irradiation induced photooxidative damage.

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