scholarly journals Vaccines and drugs against Neospora caninum, an important apicomplexan causing abortion in cattle and other farm animals [Corrigendum]

2016 ◽  
pp. 13
Author(s):  
Andrew Hemphill ◽  
Joachim Müller
Keyword(s):  
Neospora Caninum ◽  
Farm Animals

scholarly journals Neospora caninum: Structure and Fate of Multinucleated Complexes Induced by the Bumped Kinase Inhibitor BKI-1294

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 382 ◽  
Author(s):  
Pablo Winzer ◽  
Nicoleta Anghel ◽  
Dennis Imhof ◽  
Vreni Balmer ◽  
Luis-Miguel Ortega-Mora ◽  
...  
Keyword(s):  
Drug Exposure ◽  
Kinase Inhibitors ◽  
Kinase Inhibitor ◽  
Neospora Caninum ◽  
Farm Animals ◽  
In Vivo Studies ◽  
Drug Pressure ◽  
Drug Removal

Background: Bumped kinase inhibitors (BKIs) are potential drugs for neosporosis treatment in farm animals. BKI-1294 exposure results in the formation of multinucleated complexes (MNCs), which remain viable in vitro under constant drug pressure. We investigated the formation of BKI-1294 induced MNCs, the re-emergence of viable tachyzoites following drug removal, and the localization of CDPK1, the molecular target of BKIs. Methods: N. caninum tachyzoites and MNCs were studied by TEM and immunofluorescence using antibodies directed against CDPK1, and against NcSAG1 and IMC1 as markers for tachyzoites and newly formed zoites, respectively. Results: After six days of drug exposure, MNCs lacked SAG1 surface expression but remained intracellular, and formed numerous zoites incapable of disjoining from each other. Following drug removal, proliferation continued, and zoites lacking NcSAG1 emerged from the periphery of these complexes, forming infective tachyzoites after 10 days. In intracellular tachyzoites, CDPK1 was evenly distributed but shifted towards the apical part once parasites were extracellular. This shift was not affected by BKI-1294. Conclusions: CDPK1 has a dynamic distribution depending on whether parasites are located within a host cell or outside. During MNC-to-tachyzoite reconversion newly formed tachyzoites are generated directly from MNCs through zoites of unknown surface antigen composition. Further in vivo studies are needed to determine if MNCs could lead to a persistent reservoir of infection after BKI treatment.

scholarly journals Neospora caninum: Differential Proteome of Multinucleated Complexes Induced by the Bumped Kinase Inhibitor BKI-1294

2020 ◽  
Vol 8 (6) ◽  
pp. 801 ◽  
Author(s):  
Pablo Winzer ◽  
Joachim Müller ◽  
Dennis Imhof ◽  
Dominic Ritler ◽  
Anne-Christine Uldry ◽  
...  
Keyword(s):  
Kinase Inhibitors ◽  
Kinase Inhibitor ◽  
Neospora Caninum ◽  
Farm Animals ◽  
Drug Pressure ◽  
Related Sequence ◽  
Drug Candidates ◽  
Calcium Dependent ◽  
Dependent Protein

Background: the apicomplexan parasite Neospora caninum causes important reproductive problems in farm animals, most notably in cattle. After infection via oocysts or tissue cysts, rapidly dividing tachyzoites infect various tissues and organs, and in immunocompetent hosts, they differentiate into slowly dividing bradyzoites, which form tissue cysts and constitute a resting stage persisting within infected tissues. Bumped kinase inhibitors (BKIs) of calcium dependent protein kinase 1 are promising drug candidates for the treatment of Neospora infections. BKI-1294 exposure of cell cultures infected with N. caninum tachyzoites results in the formation of massive multinucleated complexes (MNCs) containing numerous newly formed zoites, which remain viable for extended periods of time under drug pressure in vitro. MNC and tachyzoites exhibit considerable antigenic and structural differences. Methods: Using shotgun mass spectrometry, we compared the proteomes of tachyzoites to BKI-1294 induced MNCs, and analyzed the mRNA expression levels of selected genes in both stages. Results: More than half of the identified proteins are downregulated in MNCs as compared to tachyzoites. Only 12 proteins are upregulated, the majority of them containing SAG1 related sequence (SRS) domains, and some also known to be expressed in bradyzoites Conclusions: MNCs exhibit a proteome different from tachyzoites, share some bradyzoite-like features, but may constitute a third stage, which remains viable and ensures survival under adverse conditions such as drug pressure. We propose the term “baryzoites” for this stage (from Greek βαρυσ = massive, bulky, heavy, inert).

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

Registration of animal rabies in Krasnodar region

2020 ◽  
pp. 3-4
Author(s):  
Oleg Yu. Chernykh ◽  
◽  
Vadim A. Bobrov ◽  
Sergey N. Zabashta ◽  
Roman A. Krivonos ◽  
...  
Keyword(s):  
Urban Form ◽  
Animal Species ◽  
Farm Animals ◽  
Natural Focus ◽  
Dead End ◽  
Wide Range ◽  
Epizootic Process ◽  
Krasnodar Region ◽  
Spread Of Infection ◽  
Reporting Data

Rabies remains a constant threat to humanity in many parts of the world. At the same time, scientifically grounded antiepizootic measures should be based on the peculiarities of the regional epizootology of this zooanthroponosis. The authors studied the epizootological and statistical reporting data of the Kropotkin Regional Veterinary Laboratory, presented an analysis of the registration of rabies in animals in Krasnodar region. From the obtained data, it should be noted that despite the wide range of animals involved in the epizootic process of rabies infection in Krasnodar region, dogs, cats and foxes play a major role in the reservation and spread of infection, which account for 78.6. Of the total number of registered cases, 15.5% falls on foxes, that indicates the natural focus of the disease, along with the manifestation of the disease in an urban form. At the same time, stray and neglected dogs and cats, which occupy a significant place among the total number of sick animals, are also sources and spread of the infection. Thus farm animals (8.3% of the total number of infected animals) are a biological dead end for the infection. Isolated cases of the disease were noted in muskrat, donkey, raccoon, raccoon dog, marten, ferret and jackal. The authors also established the specific morbidity of various animal species with rabies infection, that is an important aspect in the development and implementation of antiepizootic measures complex

Effect of CO2 on Motility of Spermatozoa of Farm Animals

1977 ◽  
Vol 48 (10) ◽  
pp. 573-575
Author(s):  
Seishiro KATO ◽  
Akira IRITANI ◽  
Yoshimasa NISHIKAWA
Keyword(s):  
Farm Animals

STRUCTURAL AND LOGISTIC MODEL OF MATERIAL FLOWS ON DIGITALLIVESTOCK FARM

2020 ◽  
pp. 26-32
Author(s):  
VLADIMIR V KIRSANOV VLADIMIR V ◽  
Keyword(s):  
Functional Model ◽  
Base Stations ◽  
Farm Animals ◽  
Biological Objects ◽  
Livestock Breeding ◽  
Video Images ◽  
Genetic Potential ◽  
Common Control ◽  
Biotechnical System ◽  
Multi Agent

The problems of the digitalization of livestock enterprises are closely related to the construction of models and algorithms describing the functioning of individual technological processes and subsystems united by a common control system. Based on the cluster approach, three groups of tasks for the intellectualization and digitalization of objects in livestock breeding are formulated: 1) recognition of images of biological objects and models of their group and individual behavior, 2) genomic assessment of farm animals, prediction of their genetic potential, with the possibility of better adaptation to technologies and specifi c economic conditions, 3) multi-agent management of automated and robotic technical means. The authors initialized the video images of biological objects, developed a structural and functional model of a complex biotechnical system “Man-Machine-Animal”, including automated workstations of key specialists, signal receiving-and-transmitting base stations, technological modules for animal service (feeding, watering, milking, microclimate, etc.), representing local biotechnical systems. The paper presents a structural-and-logistic “funnel” model of a livestock farm functioning. The model includes vectors of incoming material fl ows, outgoing production fl ows and outgoing byproducts (production waste) described using appropriate formalizations. The authors provide the structural typifi cation of technological modules and subsystems for their mathematical analysis and subsequent digital transformation of livestock farms.

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