scholarly journals A Study of hTERT Promoter Methylation in Circulating Tumour DNAs of Patients with Ovarian Magnificent Tumour

2020 ◽  
Vol Volume 13 ◽  
pp. 12317-12323
Author(s):  
Songyi Li ◽  
Wei Huang ◽  
Yinghua Li ◽  
Beibei Chen ◽  
Dingheng Li
Keyword(s):  
Promoter Methylation ◽  
Htert Promoter

Efficacy of decitabine in malignant meningioma cells: relation to promoter demethylation of distinct tumor suppressor and oncogenes and independence from TERT

2020 ◽  
pp. 1-10
Author(s):  
Louise Stögbauer ◽  
Christian Thomas ◽  
Andrea Wagner ◽  
Nils Warneke ◽  
Eva Christine Bunk ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Tumor Suppressor ◽  
Promoter Methylation ◽  
Tumor Suppressor Genes ◽  
Telomerase Activity ◽  
High Grade ◽  
Htert Promoter ◽  
Men 1 ◽  
Meningioma Cell ◽  
Tert Expression

OBJECTIVEChemotherapeutic options for meningiomas refractory to surgery or irradiation are largely unknown. Human telomerase reverse transcriptase (hTERT) promoter methylation with subsequent TERT expression and telomerase activity, key features in oncogenesis, are found in most high-grade meningiomas. Therefore, the authors investigated the impact of the demethylating agent decitabine (5-aza-2ʹ-deoxycytidine) on survival and DNA methylation in meningioma cells.METHODShTERT promoter methylation, telomerase activity, TERT expression, and cell viability and proliferation were investigated prior to and after incubation with decitabine in two benign (HBL-52 and Ben-Men 1) and one malignant (IOMM-Lee) meningioma cell line. The global effects of decitabine on DNA methylation were additionally explored with DNA methylation profiling.RESULTSHigh levels of TERT expression, telomerase activity, and hTERT promoter methylation were found in IOMM-Lee and Ben-Men 1 but not in HBL-52 cells. Decitabine induced a dose-dependent significant decrease of proliferation and viability after incubation with doses from 1 to 10 μM in IOMM-Lee but not in HBL-52 or Ben-Men 1 cells. However, effects in IOMM-Lee cells were not related to TERT expression, telomerase activity, or hTERT promoter methylation. Genome-wide methylation analyses revealed distinct demethylation of 14 DNA regions after drug administration in the decitabine-sensitive IOMM-Lee but not in the decitabine-resistant HBL-52 cells. Differentially methylated regions covered promoter regions of 11 genes, including several oncogenes and tumor suppressor genes that to the authors’ knowledge have not yet been described in meningiomas.CONCLUSIONSDecitabine decreases proliferation and viability in high-grade but not in benign meningioma cell lines. The effects of decitabine are TERT independent but related to DNA methylation changes of promoters of distinct tumor suppressor genes and oncogenes.

hTERT promoter methylation in meningiomas and central nervous hemangiopericytomas

2016 ◽  
Vol 130 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Gina Fürtjes ◽  
Michaela Köchling ◽  
Susanne Peetz-Dienhart ◽  
Andrea Wagner ◽  
Katharina Heß ◽  
...  
Keyword(s):  
Promoter Methylation ◽  
Htert Promoter

scholarly journals Exploring hTERT promoter methylation in cutaneous T‐cell lymphomas

2021 ◽  
Author(s):  
Alain Chebly ◽  
Joana Ropio ◽  
Jean‐Marie Peloponese ◽  
Sandrine Poglio ◽  
Martina Prochazkova‐Carlotti ◽  
...  
Keyword(s):  
T Cell ◽  
Promoter Methylation ◽  
Htert Promoter ◽  
T Cell Lymphomas

scholarly journals Aberrant hTERT promoter methylation predicts prognosis in Chinese patients with acral and mucosal melanoma

Medicine ◽  
2019 ◽  
Vol 98 (43) ◽  
pp. e17578
Author(s):  
Haixia Xu ◽  
Weijia Wang ◽  
Juan Zhao ◽  
Tingting Li ◽  
Xiaojing Kang
Keyword(s):  
Promoter Methylation ◽  
Mucosal Melanoma ◽  
Chinese Patients ◽  
Htert Promoter

scholarly journals hTERT promoter methylation promotes small cell lung cancer progression and radiotherapy resistance

2020 ◽  
Vol 61 (5) ◽  
pp. 674-683 ◽  
Author(s):  
Guangsheng Zhai ◽  
Jianbin Li ◽  
Jianbo Zheng ◽  
Peng An ◽  
Xiaohui Chen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Promoter Methylation ◽  
Promoter Region ◽  
Molecular Mechanisms ◽  
Small Cell ◽  
Htert Promoter ◽  
Small Cell Lung ◽  
Radiotherapy Resistance

Abstract Small cell lung cancer (SCLC) has been a devastating actuality in clinic and the molecular mechanisms underlying this disease remain unclear. The epigenetic alterations located in the promoter region of human telomerase reverse transcriptase (hTERT) have been demonstrated as one of the most prevalent non-coding genomic modifications in multiple cancers. However, alteration of hTERT promoter methylation in SCLC and the subsequently induced change in tumor cell behavior remains unclear. In this research, we hypothesized that abnormal methylation of hTERT promotor enhanced the progression of SCLC and the outcome of radiotherapy resistance. Quantitative real-time PCR and western blot assays were performed to evaluate the RNA and protein levels of hTERT and enhancer of zeste homolog 2 (EZH2), respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to estimate the viability and X-ray sensitivity of H20 and H446 cell lines. Functionally, upregulation of hTERT promoted the proliferation and migration of H20 and H446 cells, and the high-level of methylation in the promoter region of hTERT induced by radiation caused radio-resistance in SCLC. Mechanically, methylation of hTERT promoter enhanced the progression and radio-resistance of SCLC through upregulating the expression of its downstream effector EZH2.

hTERT promoter methylation in pituitary adenomas

2015 ◽  
Vol 33 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Michaela Köchling ◽  
Christian Ewelt ◽  
Gina Fürtjes ◽  
Susanne Peetz-Dienhart ◽  
Björn Koos ◽  
...  
Keyword(s):  
Promoter Methylation ◽  
Pituitary Adenomas ◽  
Htert Promoter

Abstract #60: PTEN Promoter Methylation is Specifically Detected in Differentiated Thyroid Cancers

2003 ◽  
Vol 9 ◽  
pp. 23-24
Author(s):  
Francisco Alvarez ◽  
Helena Bussaglia ◽  
Monica Vilar ◽  
Juan Ybarra ◽  
Alberto de Leiva ◽  
...  
Keyword(s):  
Promoter Methylation ◽  
Thyroid Cancers ◽  
Differentiated Thyroid Cancers
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