scholarly journals MicroRNA-1249 Targets G Protein Subunit Alpha 11 and Facilitates Gastric Cancer Cell Proliferation, Motility and Represses Cell Apoptosis

2021 ◽  
Vol Volume 14 ◽  
pp. 1249-1259
Author(s):  
Hongzhu Zhang ◽  
Tingting Fu ◽  
Cuiping Zhang
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
G Protein ◽  
Cancer Cell ◽  
Cell Apoptosis ◽  
Gastric Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Protein Subunit ◽  
Gastric Cancer Cell Proliferation

scholarly journals miR-504 Promoted Gastric Cancer Cell Proliferation and Inhibited Cell Apoptosis by Targeting RBM4

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yi Zhang ◽  
Hongmei Yong ◽  
Jing Fu ◽  
Guangyi Gao ◽  
Huichang Shi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Cell Apoptosis ◽  
Gastric Cancer Cell ◽  
Normal Group ◽  
Cancer Cell Proliferation ◽  
Gastric Cancer Cells ◽  
Gastric Cancer Cell Proliferation

Background. The purpose of this study was to explore the role and underlying mechanism of miR-504 and RBM4 in gastric cancer. Methods. The qRT-PCR or Western blot was performed to determine the expressions of miR-504 and RBM4 in the gastric cancer tissues and normal tissues. Human SGC-7901 cells were transfected with miR-504 mimic/inhibitor or pcDNA-RBM4. Cell proliferation and cell apoptosis were assessed by colony formation assay and flow cytometry, respectively. Luciferase reporter gene assays were used to investigate interactions between miR-504 and RBM4 in SGC-7901 cells. Results. The relative expression of miR-504 was significantly upregulated in the gastric cancer group ( n = 25 ) than in the paired normal group ( n = 25 ), but the relative RBM4 expression was remarkably downregulated in the gastric tumor group, compared with the normal group. Additionally, miR-504 overexpression increased the viability of gastric cancer cells. Moreover, RBM4 is a functional target of miR-504 in gastric cancer cells. miR-504 was further confirmed to promote SGC-7901 cell proliferation and inhibit cell apoptosis by downregulation RBM4 in vitro. Conclusions. miR-504 promotes gastric cancer cell proliferation and inhibits cell apoptosis by targeting RBM4, and this provides a potential diagnostic biomarker and treatment for patients with gastric cancer.

MiR-203 Affects Proliferation, Apoptosis and Cisplatin Resistance of Gastric Cancer Cells Through DJ-1-PTEN-PI3K/AKT Pathway

2019 ◽  
Vol 9 (11) ◽  
pp. 1557-1562
Author(s):  
Liangrun Zhu ◽  
Nan Zhang ◽  
Xia Shao ◽  
Ning Zhang Lili Dong ◽  
Liya Song
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cancer Cell ◽  
Cell Apoptosis ◽  
Gastric Cancer Cell ◽  
Cancer Drug ◽  
Akt Pathway ◽  
Cancer Cell Proliferation ◽  
Gastric Cancer Cells ◽  
Gastric Cancer Cell Proliferation

DJ-1 negatively regulates phosphatase and tensin homolog gene (PTEN). Abnormal miR-203 expression is associated with gastric cancer. Bioinformatics analysis showed a targeting relationship between miR-203 and DJ-1 3′-UTR. Our study evaluated the role of miR-203 gastric cancer cell proliferation, apoptosis, and cisplatin (DDP) resistance. The CDDP-resistant cell line SGC7901/CDDP was established and divided into miR-NC group and miR-203 mimic group followed by detecting DJ-1, PTEN and p-AKT expression, and cell apoptosis and proliferation by flow cytometry. There was a targeted relationship between miR-203 and DJ-1 mRNA. miR-203 expression in SGC7901/CDDP cells was significantly reduced compared with SGC7901 cells with elevated DJ-1 mRNA and protein level. Compared with miR-NC group, DJ-1 and p-AKT in SGC7901/CDDP cells was significantly downregulated in miR-203 mimic transfection group, while PTEN expression was significantly increased, cell apoptosis was increased, and proliferation and CDDP resistance were reduced. Decreased miR-203 and increased DJ-1 level is associated with gastric cancer drug resistance. Elevated miR-203 can downregulate DJ-1, affect the activity of PTEN-PI3K/AKT pathway, inhibit gastric cancer cell proliferation, promote cell apoptosis, and enhance the drug sensitivity to CDDP.

scholarly journals Carnosic Acid Induces Apoptosis and Inhibits Akt/mTOR Signaling in Human Gastric Cancer Cell Lines

2021 ◽  
Vol 14 (3) ◽  
pp. 230
Author(s):  
Waseem El-Huneidi ◽  
Khuloud Bajbouj ◽  
Jibran Sualeh Muhammad ◽  
Arya Vinod ◽  
Jasmin Shafarin ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Carnosic Acid ◽  
Human Gastric Cancer ◽  
Cancer Cell Proliferation ◽  
Human Gastric Cancer Cell ◽  
Gastric Cancer Cell Lines ◽  
Gastric Cancer Cell Proliferation

Gastric cancer is among the most common malignancies worldwide. Due to limited availability of therapeutic options, there is a constant need to find new therapies that could target advanced, recurrent, and metastatic gastric cancer. Carnosic acid is a naturally occurring polyphenolic abietane diterpene derived from Rosmarinus officinalis and reported to have numerous pharmacological effects. In this study, the cytotoxicity assay, Annexin V-FITC/PI, caspases 3, 8, and 9, cell cycle analysis, and Western blotting were used to assess the effect of carnosic acid on the growth and survival of human gastric cancer cell lines (AGS and MKN-45). Our findings showed that carnosic acid inhibited human gastric cancer cell proliferation and survival in a dose-dependent manner. Additionally, carnosic acid is found to inhibit the phosphorylation/activation of Akt and mTOR. Moreover, carnosic acid enhanced the cleavage of PARP and downregulated survivin expression, both being known markers of apoptosis. In conclusion, carnosic acid exhibits antitumor activity against human gastric cancer cells via modulating the Akt-mTOR signaling pathway that plays a crucial role in gastric cancer cell proliferation and survival.

scholarly journals Reduction of Hip2 suppresses gastric cancer cell proliferation, migration, invasion and tumorigenesis

2020 ◽  
Vol 9 (2) ◽  
pp. 774-785
Author(s):  
Jugang Wu ◽  
Baoxing Tian ◽  
Jianjun Yang ◽  
Haizhong Huo ◽  
Zhicheng Song ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Gastric Cancer Cell Proliferation
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