scholarly journals High Centromere Protein-A (CENP-A) Expression Correlates with Progression and Prognosis in Gastric Cancer

2020 ◽  
Vol Volume 13 ◽  
pp. 13237-13246
Author(s):  
Yuan Xu ◽  
Chao Liang ◽  
Xianlei Cai ◽  
Miaozun Zhang ◽  
Weiming Yu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Protein A ◽  
Centromere Protein ◽  
Centromere Protein A

Anti-centromere protein A antibodies in systemic sclerosis: Significance and origin

2016 ◽  
Vol 15 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Federico Perosa ◽  
Marcella Prete ◽  
Giuseppe Di Lernia ◽  
Carmela Ostuni ◽  
Elvira Favoino ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Protein A ◽  
Centromere Protein ◽  
Centromere Protein A

scholarly journals Functional Complementation of Human Centromere Protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae

2004 ◽  
Vol 24 (15) ◽  
pp. 6620-6630 ◽  
Author(s):  
Gerhard Wieland ◽  
Sandra Orthaus ◽  
Sabine Ohndorf ◽  
Stephan Diekmann ◽  
Peter Hemmerich
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Budding Yeast ◽  
Protein A ◽  
Human Cells ◽  
Cycle Arrest ◽  
Centromere Protein ◽  
Centromere Protein A

ABSTRACT We have employed a novel in vivo approach to study the structure and function of the eukaryotic kinetochore multiprotein complex. RNA interference (RNAi) was used to block the synthesis of centromere protein A (CENP-A) and Clip-170 in human cells. By coexpression, homologous kinetochore proteins from Saccharomyces cerevisiae were then tested for the ability to complement the RNAi-induced phenotypes. Cse4p, the budding yeast CENP-A homolog, was specifically incorporated into kinetochore nucleosomes and was able to complement RNAi-induced cell cycle arrest in CENP-A-depleted human cells. Thus, Cse4p can structurally and functionally substitute for CENP-A, strongly suggesting that the basic features of centromeric chromatin are conserved between yeast and mammals. Bik1p, the budding yeast homolog of human CLIP-170, also specifically localized to kinetochores during mitosis, but Bik1p did not rescue CLIP-170 depletion-induced cell cycle arrest. Generally, the newly developed in vivo complementation assay provides a powerful new tool for studying the function and evolutionary conservation of multiprotein complexes from yeast to humans.

scholarly journals Centromere protein A dynamics in human pluripotent stem cell self-renewal, differentiation and DNA damage

2010 ◽  
Vol 19 (20) ◽  
pp. 3970-3982 ◽  
Author(s):  
Gayane Ambartsumyan ◽  
Rajbir K. Gill ◽  
Silvia Diaz Perez ◽  
Deirdre Conway ◽  
John Vincent ◽  
...  
Keyword(s):  
Dna Damage ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Protein A ◽  
Human Pluripotent Stem Cell ◽  
Self Renewal ◽  
Centromere Protein ◽  
Centromere Protein A

scholarly journals Anticentromere antibody induced by immunization with centromere protein a and Freund’s complete adjuvant may interfere with mouse oocyte meiosis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ying Ying ◽  
Shuang Liu ◽  
Yixuan Wu ◽  
Sichen Li ◽  
Qing Huang
Keyword(s):  
Protein A ◽  
Underlying Mechanism ◽  
Saline Group ◽  
Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Groups ◽  
Anticentromere Antibody ◽  
Centromere Protein ◽  
Centromere Protein A ◽  
Oocyte Meiosis

Abstract Background Anticentromere antibody (ACA) is a member of the antinuclear antibody (ANA) family, and recent studies have found that ACA may be associated with oocyte maturation disorders; however, the possible mechanism behind this phenomenon remains unknown. We conducted this study to investigate whether ACA could penetrate into the living oocytes and interfere with oocyte meiosis in a mouse model. Methods We divided mice into three groups: human recombinant centromere protein-A (human CENP-A, HA) and complete Freund’s adjuvant (CFA) were used to immunize mice for the study group (HA + CFA), and mice injected with CFA (CFA group) or saline (Saline group), respectively, served as controls. After immunization, serum anti-CENP-A antibody was detected by indirect immunofluorescence assay (IIFT) and enzyme-linked immunosorbent assay (ELISA). Chromosome alignment and intracellular IgG localization in MI- and MII-stage oocytes were investigated by immunofluorescence analysis. Results Positive ACAs were successfully induced by immunization with CENP-A and CFA, and results showed that the serum level of anti-CENP-A antibody was significantly higher in the HA + CFA group compared with the control groups. There was marked increase of chromosome misalignments in MI and MII oocytes in the HA + CFA group compared to the control groups. However, no oocytes from any of the three groups showed intracellular antibody immunofluorescence. Conclusions The development and maturation of oocytes were impaired in peripheral ACA positive mice, which exhibited severe chromosomal misalignments in metaphase meiosis; however, no evidence of ACAs entering the oocytes was observed, thus the underlying mechanism needs further exploration.

scholarly journals Anticentromere antibody induced by immunization with centromere protein and Freund’s complete adjuvant may interfere with mouse early-stage embryo

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hanyan Liu ◽  
Yufen Zhang ◽  
Haiying Liu ◽  
Qing Huang ◽  
Ying Ying
Keyword(s):  
Early Stage ◽  
Protein A ◽  
Immunofluorescence Assay ◽  
Immunofluorescence Test ◽  
Anticentromere Antibody ◽  
Indirect Immunofluorescence Test ◽  
Centromere Protein ◽  
Centromere Protein A ◽  
Stage Embryo ◽  
Early Stage Embryo

Abstract Background Anticentromere antibody (ACA) is a member of the antinuclear antibody spectrum (ANAs) which has been speculated to be associated with subfertility. Thus, the present study aimed to investigate the induction of ACA production and its potential interference with early-stage embryos. Methods Recombinant centromere protein-A (CENP-A) or centromere protein-B (CENP-B) and complete Freund’s adjuvant (CFA) were used to immunize mice. Serum ACA level was then evaluated by using an indirect immunofluorescence test. Immunofluorescence assay was performed to detect IgG in follicles in ovarian tissues and early-stage embryos. Results Following treatment, serum positive ACA was observed in mice treated with CENP and CFA. Furthermore, IgG were detected in follicular fluid and early-stage embryos from mice treated with CENP and CFA. Conclusions This study preliminarily indicated that ACA induced by CENP and CFA may penetrate into the living embryos of early-stage in mice.

Centromere protein A goes far beyond the centromere in cancers

2021 ◽  
pp. molcanres.0311.2021
Author(s):  
Xiaolan Liu ◽  
Haiping Wang ◽  
Guojun Zhao
Keyword(s):  
Protein A ◽  
Centromere Protein ◽  
Centromere Protein A

scholarly journals Uracil DNA N-Glycosylase Promotes Assembly of Human Centromere Protein A

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17151 ◽  
Author(s):  
Samantha G. Zeitlin ◽  
Brian R. Chapados ◽  
Norman M. Baker ◽  
Caroline Tai ◽  
Geir Slupphaug ◽  
...  
Keyword(s):  
Protein A ◽  
Human Centromere ◽  
Centromere Protein ◽  
Centromere Protein A
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