scholarly journals Cas9 Mediated Correction of β-catenin Mutation and Restoring the Expression of Protein Phosphorylation in Colon Cancer HCT-116 Cells Decrease Cell Proliferation in vitro and Hamper Tumor Growth in Mice in vivo

2020 ◽  
Vol Volume 13 ◽  
pp. 17-29 ◽  
Author(s):  
Yanlan Li ◽  
Xiangning Li ◽  
Jiayao Qu ◽  
Dixian Luo ◽  
Zheng Hu
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Tumor Growth ◽  
Protein Phosphorylation ◽  
Decrease Cell Proliferation ◽  
Hct 116 ◽  
Hct 116 Cells ◽  
Proliferation In Vitro

T1374 EZH2 Knock Down by Lentiviral shRNA Inhibits Pancreatic Ductal Adeno-Carcinoma Cell Proliferation In Vitro and Tumor Growth In Vivo

2010 ◽  
Vol 138 (5) ◽  
pp. S-548
Author(s):  
Ramesh B. Batchu ◽  
Aamer Qazi ◽  
Shelly Seward ◽  
Masood A. Shammas ◽  
Sreedhar Chamala ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Carcinoma Cell ◽  
Knock Down ◽  
Adeno Carcinoma ◽  
Proliferation In Vitro

scholarly journals Targeting MYC and HDAC8 with a Combination of siRNAs Inhibits Neuroblastoma Cells Proliferation In Vitro and In Vivo Xenograft Tumor Growth

2021 ◽  
Author(s):  
Nagindra Prashad
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Cellular Differentiation ◽  
Tumor Model ◽  
Significant Inhibition ◽  
Luciferase Reporter Plasmid ◽  
Combination Of Sirnas ◽  
Proliferation In Vitro

HDAC8, c MYC and MYCN are involved in the tumorigenesis of neuroblastoma. A mouse Neuroblastoma (NB) tumor model was used to understand the role of miRNA, miR-665 in NB tumorigenesis and cellular differentiation. During cellular differentiation of NB cells there is an up regulated miRNA-665. We found that HDAC 8, c MYC and MYCN are the direct targets of mimic miR-665 which was validated by luciferase reporter plasmid with 3’ UTR and ELISA. Mimic miR-665 inhibited cell proliferation, arrested cells in G1 stage and decreased S Phase in cell cycle. miR-665 increased the acetylation of histones and activated Caspase 3. This is the first report to recognize miRNA 665 as a suppressor miRNA of NB. The effects of miR-665 were confirmed with the transfection of siRNA for HDAC8 and siRNA for MYC. Individual siRNA- HDAC8 or siRNA-MYC inhibited 40–50% of cell proliferation in vitro, however, the treatment with the combination of both siRNA-MYC + siRNA- HDAC8 inhibited 86% of cell proliferation. Indicating that both the targets c MYC and HDAC 8 should be reduced to obtain a significant inhibition of cell proliferation. Intratumoral treatment of xenograft tumors in mice with the combination of siRNA-MYC + siRNA- HDAC8 reduced the levels of target c-MYC protein by 64% and target HDAC 8 protein by 85% and the average tumor growth reduced by 80% compared to control tumors treated with NC-siRNA. Our results suggest the potential therapeutic effect of suppressor miR-665 and the combination of siRNA-MYC + siRNA-HDAC8 for neuroblastoma treatment.

Low-intensity ultrasound inhibits melanoma cell proliferation in vitro and tumor growth in vivo

2021 ◽  
Author(s):  
Loreto B. Feril ◽  
Kazuki Yamaguchi ◽  
Yurika Ikeda-Dantsuji ◽  
Yukihiro Furusawa ◽  
Yoshiaki Tabuchi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Melanoma Cell ◽  
Low Intensity ◽  
Melanoma Cell Proliferation ◽  
Low Intensity Ultrasound ◽  
Proliferation In Vitro

Inhibition of Cancer Cell Proliferation in vitro and Tumor Growth in vivo by Hydrophis spiralis Sea Snake Venom

2007 ◽  
Vol 3 (4) ◽  
pp. 186-190
Author(s):  
R. Karthikeya ◽  
S. Karthigaya ◽  
M. Sri Balasubash ◽  
S. Vijayalaks ◽  
S.T. Somasundar ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Cancer Cell ◽  
Snake Venom ◽  
Cancer Cell Proliferation ◽  
Sea Snake ◽  
Proliferation In Vitro

Calreticulin and Calreticulin Fragments Are Endothelial Cell Inhibitors That Suppress Tumor Growth

Blood ◽  
1999 ◽  
Vol 94 (7) ◽  
pp. 2461-2468 ◽  
Author(s):  
Sandra E. Pike ◽  
Lei Yao ◽  
Joyce Setsuda ◽  
Karen D. Jones ◽  
Barry Cherney ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Endothelial Cell ◽  
Tumor Growth ◽  
Angiogenesis Inhibitors ◽  
Full Length ◽  
Endothelial Cell Proliferation ◽  
Proliferation In Vitro

Several angiogenesis inhibitors are fragments of larger proteins that are themselves not active as angiogenesis inhibitors. Vasostatin, the N-terminal domain of calreticulin inclusive of amino acids 1-180, is an angiogenesis inhibitor that exerts antitumor effects in vivo. In the present study, we examined whether the full-length calreticulin molecule shares the antiangiogenic and antitumor activities of vasostatin. Similar to vasostatin, calreticulin selectively inhibited endothelial cell proliferation in vitro, but not cells of other lineages, and suppressed angiogenesis in vivo. When inoculated into athymic mice, calreticulin inhibited Burkitt tumor growth comparably with vasostatin. Calreticulin lacking the N-terminal 1-120 amino acids inhibited endothelial cell proliferation in vitro and Burkitt tumor growth in vivo comparably with vasostatin. An internal calreticulin fragment encompassing amino acids 120-180 also inhibited endothelial cell proliferation in vitro and angiogenesis in vivo comparably with calreticulin and vasostatin. These results suggest that the antiangiogenic activities of vasostatin reside in a domain that is accessible from the full-length calreticulin molecule and localize to calreticulin N-terminal amino acids 120-180. Thus, calreticulin and calreticulin fragments are inhibitors of angiogenesis that directly target endothelial cells, inhibit angiogenesis, and suppress tumor growth. This information may be critical in designing targeted inhibitors of pathological angiogenesis that underlies cancer and other diseases.

Inhibition of Hep2 and HeLa cell proliferation in vitro and EAC tumor growth in vivo by Lapemis curtus (Shaw 1802) venom

Toxicon ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 157-161 ◽  
Author(s):  
R. Karthikeyan ◽  
S. Karthigayan ◽  
M. Sri Balasubashini ◽  
S.T. Somasundaram ◽  
T. Balasubramanian
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Hela Cell ◽  
Proliferation In Vitro

Inhibition of mammary carcinoma cell proliferation in vitro and tumor growth in vivo by bee venom

Toxicon ◽  
2003 ◽  
Vol 41 (7) ◽  
pp. 861-870 ◽  
Author(s):  
Nada Oršolić ◽  
Lidija Šver ◽  
Srđan Verstovšek ◽  
Svjetlana Terzić ◽  
Ivan Bašić
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Mammary Carcinoma ◽  
Carcinoma Cell ◽  
Bee Venom ◽  
Mammary Carcinoma Cell ◽  
Proliferation In Vitro

scholarly journals Coptisine from Rhizoma Coptidis Suppresses HCT-116 Cells-related Tumor Growth in vitro and in vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Tao Huang ◽  
Yubo Xiao ◽  
Lin Yi ◽  
Ling Li ◽  
Meimei Wang ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Rhizoma Coptidis ◽  
Hct 116 ◽  
Hct 116 Cells

scholarly journals Alpha‐mangostin reduces HT‐29 colon cancer cell proliferation in vitro and inhibits transplantable tumorigenesis in vivo.

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Chureeporn Chitchumroonchokchai ◽  
Sunit Suksumrarn ◽  
Jun‐ge Yu ◽  
Steven K. Clinton ◽  
Mark L. Failla
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Proliferation In Vitro ◽  
Ht 29
Sign in / Sign up

Export Citation Format

Share Document