scholarly journals C-terminal of E1A binding protein 1 enhances the migration of gastric epithelial cells and has a clinicopathologic significance in human gastric carcinoma

2019 ◽  
Vol Volume 12 ◽  
pp. 5189-5200
Author(s):  
Can Wang ◽  
Min Wang ◽  
Baocheng Xing ◽  
Zhaocheng Chi ◽  
Hongyu Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Gastric Carcinoma ◽  
Binding Protein ◽  
Gastric Epithelial Cells ◽  
Human Gastric Carcinoma

scholarly journals High-throughput sequencing reveals circular RNA hsa_circ_0000592 as a novel player in the carcinogenesis of gastric carcinoma

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Min Liang ◽  
Zhaoyu Liu ◽  
Hai Lin ◽  
Boyun Shi ◽  
Ming Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
T Cells ◽  
Epithelial Cells ◽  
Gastric Carcinoma ◽  
Cell Lines ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Gastric Epithelial Cells ◽  
Environmental Carcinogen ◽  
Functional Roles

Abstract Background/Aim: Gastric cancer is one of the most common malignant tumors, and its complex pathogenesis has not been fully elucidated. Circular RNAs (circRNAs) are involved in various biological processes and human diseases. However, their exact functional roles and mechanisms of action remain largely unclear. We previously discovered the differential expression of non-coding RNAs (ncRNAs) during the malignant transformation of human gastric epithelial cells. In this study, we investigated the functional roles of a significantly up-regulated circRNA (hsa_circ_0000592) in gastric cancer. Methods:N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced malignant-transformed gastric epithelial cells (GES-1-T) and normal gastric epithelial cells (GES-1-N) were analyzed by high-throughput circRNA sequencing. The top 15 up-regulated circRNAs in high-throughput sequencing results were further confirmed by qRT-PCR in different gastric epithelial cell lines. The function of the most significant circRNA (hsa_circ_0000592) was investigated by using RNA interference (RNAi) assays, fluorescence in situ hybridization analysis (FISH), and bioinformatics prediction methods. Results: A total of 1509 genes were up-regulated and 3142 genes were down-regulated in GES-1-T cells when compared with GES-1-N cells. When compared with GES-1-N cells, hsa_circ_0000592 was obviously up-regulated in GES-1-T cells, as well as in other gastric cancer cell lines. The silencing of hsa_circ_0000592 mRNA led to a decrease in cell proliferation, cell cycle arrest at the G0/G1 phase, an increased rate of apoptosis, and a reduction in cell migration. Furthermore, FISH showed that hsa_circ_0000592 was mainly located in the cytoplasm, and a bioinformatics analysis suggested that hsa_circ_0000592 might function by sponging multiple miRNAs, and most notably four conserved miRNAs, including miR-139-3p, miR-200, miR-367-3p, and miR-33a-3p. Conclusion: This study is the first to identify hsa_circ_0000592 as a novel circRNA with a critical role in MNNG-induced gastric cancer. Due to the essential role of hsa_circ_0000592 in gastric carcinoma cells, it may be considered as a potential biomarker for use in diagnosing gastric carcinoma. Our findings provide a new insight into the function of circRNAs in environmental carcinogen-induced gastric cancer.

scholarly journals Interaction of Early Growth Response Protein 1 (Egr-1), Specificity Protein 1 (Sp1), and Cyclic Adenosine 3′5′-Monophosphate Response Element Binding Protein (CREB) at a Proximal Response Element Is Critical for Gastrin-Dependent Activation of the Chromogranin A Promoter

2002 ◽  
Vol 16 (12) ◽  
pp. 2802-2818 ◽  
Author(s):  
Raktima Raychowdhury ◽  
Georgia Schäfer ◽  
John Fleming ◽  
Stefan Rosewicz ◽  
Bertram Wiedenmann ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Chromogranin A ◽  
Promoter Activity ◽  
Growth Response ◽  
Binding Protein ◽  
Early Growth ◽  
Gastric Epithelial Cells ◽  
Response Element ◽  
Early Growth Response ◽  
Element Binding Protein

Abstract Recently, binding of specific protein 1 (Sp1) and cAMP response element binding protein (CREB) to a GC-rich element at −92/−62 has been identified as a critical step in gastrin-dependent regulation of the chromogranin A (CgA) gene in gastric epithelial cells. Here we demonstrate that binding of early growth response protein 1 (Egr-1) to the distal part of the −92/−62 site is also required for gastrin-dependent CgA transactivation. Gastrin elevated cellular and nuclear Egr-1 levels in a time-dependent manner and also increased Egr-1 binding to the CgA −92/−73 region. Disruption of this site reduced gastrin responsiveness without influencing basal promoter activity, while loss of Sp1 and/or CREB binding sites diminished basal and gastrin-stimulated CgA promoter activity. Ectopic Egr-1 overexpression potently stimulated the CgA promoter, whereas coexpression of Egr-1 with Sp1 and/or CREB resulted in additive effects. Functional analysis of Sp1-, Egr-1-, or CREB-specific promoter mutations in transfection studies confirmed the tripartite organization of the CgA −92/−62 element. Signaling studies revealed that MAPK kinase 1 (MEK1)/ERK1/2 cascades are critical for gastrin-dependent Egr-1 protein accumulation as well as Egr-1 binding to the CgA promoter. Our studies for the first time identify Egr-1 as a nuclear target of gastrin and show that functional interplay of Egr-1, Sp1, and CREB is indispensable for gastrin-dependent CgA transactivation in gastric epithelial cells.

Effect of H. pylori on the expression of TRAIL receptor subtypes and apoptosis in human gastric epithelial cells

2001 ◽  
Vol 120 (5) ◽  
pp. A81-A81
Author(s):  
J MARTIN ◽  
A POTTHOFF ◽  
M COMBERG ◽  
I SOBEKKLOCKE ◽  
S LEDIG ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Receptor Subtypes ◽  
Trail Receptor ◽  
Gastric Epithelial Cells ◽  
H Pylori

Mechanistic study of proliferation induced by Angelica sinensis in gastric epithelial cells

2001 ◽  
Vol 120 (5) ◽  
pp. A145-A145
Author(s):  
C CHO ◽  
Y YE ◽  
E LIU ◽  
V SHIN ◽  
N SHAM
Keyword(s):  
Epithelial Cells ◽  
Mechanistic Study ◽  
Angelica Sinensis ◽  
Gastric Epithelial Cells

Gastrin induces IL-8 expression in gastric epithelial cells through the activation of NF-kappaB and AP-1

2001 ◽  
Vol 120 (5) ◽  
pp. A727-A727
Author(s):  
Y MIYAZAKI ◽  
S HIRAOKA ◽  
S KITAMURA ◽  
M TOYOTA ◽  
T KIYOHARA ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Gastric Epithelial Cells ◽  
Nf Kappab
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