scholarly journals Inhibition of GLUT-1 expression and the PI3K/Akt pathway to enhance the chemosensitivity of laryngeal carcinoma cells in vitro

2018 ◽  
Vol Volume 11 ◽  
pp. 7865-7872 ◽  
Author(s):  
Tao Jiang ◽  
Min-Li Zhou ◽  
Jun Fan
Keyword(s):  
Laryngeal Carcinoma ◽  
Carcinoma Cells ◽  
Akt Pathway ◽  
Glut 1

scholarly journals Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In Vitro

2011 ◽  
Vol 62 (2) ◽  
pp. 139-146 ◽  
Author(s):  
Ksenija Durgo ◽  
Sandra Kostić ◽  
Katarina Gradiški ◽  
Draženka Komes ◽  
Maja Osmak ◽  
...  
Keyword(s):  
Cell Line ◽  
Green Tea ◽  
Laryngeal Carcinoma ◽  
Resistant Cell ◽  
Resistant Cell Line ◽  
Green Tea Extract ◽  
Carcinoma Cells ◽  
Tea Extract ◽  
Human Laryngeal Carcinoma

Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In VitroGreen tea (Camellia sinensis) contains several bioactive compounds which protect the cell and prevent tumour development. Phytochemicals in green tea extract (mostly flavonoids) scavenge free radicals, but also induce pro-oxidative reactions in the cell. In this study, we evaluated the potential cytotoxic and prooxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) on human laryngeal carcinoma cell line (HEp2) and its cross-resistant cell line CK2. The aim was to see if the extract and its two flavonoids could increase the sensitivity of the cisplatin-resistant cell line CK2 in comparison to the parental cell line. The results show that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. The pro-oxidant effect of green tea was determined at concentrations higher than those found in traditionally prepared green tea infusions.

scholarly journals The Silencing of Pokemon Attenuates the Proliferation of Hepatocellular Carcinoma Cells In Vitro and In Vivo by Inhibiting the PI3K/Akt Pathway

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51916 ◽  
Author(s):  
Chan-Chan Lin ◽  
Jing-Ping Zhou ◽  
Yun-Peng Liu ◽  
Jing-Jing Liu ◽  
Xiao-Ning Yang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Carcinoma Cells ◽  
Akt Pathway ◽  
Hepatocellular Carcinoma Cells

Silencing of Src by siRNA inhibits laryngeal carcinoma growth through the Src/PI-3 K/Akt pathway in vitro and in vivo

Tumor Biology ◽  
2014 ◽  
Vol 35 (9) ◽  
pp. 9009-9014 ◽  
Author(s):  
Yan Song ◽  
Yao-dong Dong ◽  
Wei-liang Bai ◽  
Xiu-lan Ma
Keyword(s):  
Laryngeal Carcinoma ◽  
Akt Pathway

Construction and Identification of an Antisense Glucose Transporter-1 Plasmid

2008 ◽  
Vol 36 (5) ◽  
pp. 1001-1007
Author(s):  
XM Chen ◽  
SH Zhou ◽  
J Fan ◽  
MJ Hu ◽  
SQ Wang ◽  
...  
Keyword(s):  
Laryngeal Carcinoma ◽  
Glucose Transporter ◽  
Therapeutic Potential ◽  
Restriction Analysis ◽  
Expression System ◽  
Carcinoma Cells ◽  
Glucose Transporter 1 ◽  
Glut 1 ◽  
Firm Basis ◽  
Polymerase Chain

Our aim was to construct a pcDNA3.1(+) eucaryotic expression system vector containing the antisense glucose transporter-1 ( Glut-1) gene. Total RNA was isolated from human Hep-2 laryngeal carcinoma cells, and the Glut-1 and antisense Glut-1 sequences were amplified by polymerase chain reaction. Expression plasmids containing the sense and antisense cDNA were constructed using the pcDNA3.1(+) vector. The resulting sense and antisense vectors, pcDNA3.1(+)-Glut-1 and pcDNA3.1(+)-antiGlut-1, respectively, were examined by restriction analysis and DNA sequencing. The pcDNA3.1(+)-antiGlut-1 was subsequently transfected into Hep-2 cells. Anti Glut-1 mRNA expression was detected, indicating the successful construction of an antisense Glut-1 plasmid capable of transfecting Hep-2 laryngeal carcinoma cells. These data provide a firm basis for additional studies using the plasmid pcDNA3.1(+)-antiGlut-1 to determine its therapeutic potential for the treatment of laryngeal carcinoma.

scholarly journals Alpinia oxyphylla oil induces apoptosis of hepatocellular carcinoma cells via PI3K/Akt pathway in vitro and in vivo

2019 ◽  
Vol 109 ◽  
pp. 2365-2374 ◽  
Author(s):  
Fuhai Hui ◽  
Xiaochun Qin ◽  
Qiao Zhang ◽  
Ruolan Li ◽  
Mingyue Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Carcinoma Cells ◽  
Akt Pathway ◽  
Hepatocellular Carcinoma Cells ◽  
Alpinia Oxyphylla
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