scholarly journals miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples

2017 ◽  
Vol Volume 10 ◽  
pp. 4225-4238 ◽  
Author(s):  
Ovidiu-Leonard Braicu ◽  
Liviuta Budisan ◽  
Rares Buiga ◽  
Ancuta Jurj ◽  
Patriciu Achimas-Cadariu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Mirna Expression ◽  
Expression Profiling ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Mirna Expression Profiling ◽  
Formalin Fixed

scholarly journals miRNA expression profiling of formalin-fixed paraffin-embedded (FFPE) hereditary breast tumors

Genomics Data ◽  
2015 ◽  
Vol 3 ◽  
pp. 75-79 ◽  
Author(s):  
Miljana Tanić ◽  
Kira Yanowski ◽  
Eduardo Andrés ◽  
Gonzalo Gómez-López ◽  
María Rodríguez-Pinilla Socorro ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Expression Profiling ◽  
Breast Tumors ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Mirna Expression Profiling ◽  
Formalin Fixed

scholarly journals Expression profiling of formalin-fixed paraffin embedded primary human uveal melanomas using DASL matrices

2009 ◽  
Vol 136 (4) ◽  
pp. 577-586 ◽  
Author(s):  
Sebastian Di Cesare ◽  
Andre Nantel ◽  
Jean-Claude Marshall ◽  
Bruno F. Fernandes ◽  
Emilia Antecka ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Uveal Melanomas

Expression of proliferation genes in formalin-fixed paraffin-embedded (FFPE) tissue from breast carcinomas. Feasibility and relevance for a routine histopathology laboratory

2016 ◽  
Vol 70 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Carla Thomas ◽  
Cleo Robinson ◽  
Ben Dessauvagie ◽  
Benjamin Wood ◽  
Greg Sterrett ◽  
...  
Keyword(s):  
Gene Expression ◽  
Breast Carcinoma ◽  
Expression Profiling ◽  
Proliferative Activity ◽  
Breast Cancers ◽  
Breast Carcinomas ◽  
Ki 67 ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

AimBreast carcinoma proliferative activity, histological grade and commercial molecular tests are all important in prognostication and treatment. There is a particular need for improved, standardised techniques for subclassification of grade 2 breast cancers into low-risk and high-risk prognostic groups. In this study we investigated whether gene expression profiling of five proliferation genes was feasible using breast cancer tissue in a clinical setting and whether these profiles could enhance pathological assessment.MethodsExpression of five proliferation gene mRNAs; Ki-67, STK 15, CCNB1, CCND1 and MYBL2, was quantified in 27 breast carcinomas and compared with Ki-67 proliferation index (PI) and Nottingham mitotic score.ResultsExpression of Ki-67, STK15 and MYBL2 mRNA showed moderate Spearman's correlation with Ki-67 PI (p<0.01), but CCND1 and CCNB1 showed weak, non-significant correlation. Individual gene expression did not associate with mitotic score but combined mRNA expression correlated with both Ki-67 PI (p=0.018) and mitotic score (p=0.03; 0.007).ConclusionsThis study confirms mRNA analysis in breast carcinoma formalin-fixed, paraffin-embedded samples is feasible and suggests gene expression profiling, using a small set of five proliferation genes, has potential in aiding histological grading or assessment of proliferative activity of breast cancers. To fully evaluate the clinical applicability of this approach, a larger cohort study with long-term follow-up data is required.

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