scholarly journals Inhibitory effect of quercetin on epithelial to mesenchymal transition in SK-MEL-28 human melanoma cells defined by in vitro analysis on 3D collagen gels

2016 ◽  
Vol Volume 9 ◽  
pp. 6445-6459 ◽  
Author(s):  
Dhairya Patel ◽  
Neeti Sharma
Keyword(s):  
Epithelial To Mesenchymal Transition ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Collagen Gels ◽  
Mesenchymal Transition ◽  
3D Collagen ◽  
Vitro Analysis ◽  
In Vitro Analysis

Dexamethasone-dependent versus -independent markers of epithelial to mesenchymal transition in primary hepatocytes

2010 ◽  
Vol 391 (1) ◽  
Author(s):  
Patricio Godoy ◽  
Sumathi Lakkapamu ◽  
Markus Schug ◽  
Alexander Bauer ◽  
Joanna D. Stewart ◽  
...  
Keyword(s):  
Epithelial To Mesenchymal Transition ◽  
Culture Media ◽  
Three Dimensional ◽  
Morphological Features ◽  
Collagen Gels ◽  
Mesenchymal Transition ◽  
Subsequent Step ◽  
Twist 1 ◽  
Emt Markers

Abstract Recently, epithelial to mesenchymal transition (EMT) has been shown to represent a feature of dedifferentiating hepatocytes in vitro. Three-dimensional soft collagen gels can antagonize but not completely abolish this effect. Hormonal additives to culture media are known to maintain differentiated hepatocyte functions. Therefore, we studied whether insulin and dexamethasone antagonize EMT in cultured hepatocytes. Both hormones antagonized but not completely abolished certain morphological features of EMT. Dexamethasone antagonized acquisition of fibroblastoid shape, whereas insulin favored bile canaliculi formation. In a subsequent step, we analyzed expression of a battery of EMT-related genes. Of all markers tested, vimentin and snail-1 correlated best with morphological features of EMT. Interestingly, dexamethasone reduced expression levels of both vimentin and snail-1, whereas the influence of insulin was less pronounced. An important result of this study is that 12 out of 17 analyzed EMT markers were transcriptionally influenced by dexamethasone (vimentin, snail-1, snail-2, HNF4α, Twist-1, ZEB2, fibronectin, occludin, MMP14, claudin-1, cytokeratin-8, and cytokeratin-18), whereas the remaining factors seemed to be less dependent on dexamethasone. In conclusion, EMT markers in hepatocytes can be classified as dexamethasone-dependent versus -independent.

scholarly journals CD63 Tetraspanin Is a Negative Driver of Epithelial-to-Mesenchymal Transition in Human Melanoma Cells

2014 ◽  
Vol 134 (12) ◽  
pp. 2947-2956 ◽  
Author(s):  
Antonella Lupia ◽  
Silvia Peppicelli ◽  
Ewa Witort ◽  
Francesca Bianchini ◽  
Vinicio Carloni ◽  
...  
Keyword(s):  
Epithelial To Mesenchymal Transition ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Mesenchymal Transition ◽  
Human Melanoma Cells

scholarly journals From Proteomic Mapping to Invasion-Metastasis-Cascade Systemic Biomarkering and Targeted Drugging of Mutant BRAF-Dependent Human Cutaneous Melanomagenesis

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2024
Author(s):  
Aikaterini F. Giannopoulou ◽  
Athanassios D. Velentzas ◽  
Athanasios K. Anagnostopoulos ◽  
Adamantia Agalou ◽  
Nikos C. Papandreou ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Epithelial To Mesenchymal Transition ◽  
Hypoxia Inducible Factor ◽  
Primary Melanoma ◽  
Melanoma Cells ◽  
Mesenchymal Transition ◽  
Mesenchymal To Epithelial Transition ◽  
Liquid Chromatography Tandem Mass ◽  
Novel Biomarkers

Melanoma is classified among the most notoriously aggressive human cancers. Despite the recent progress, due to its propensity for metastasis and resistance to therapy, novel biomarkers and oncogenic molecular drivers need to be promptly identified for metastatic melanoma. Hence, by employing nano liquid chromatography-tandem mass spectrometry deep proteomics technology, advanced bioinformatics algorithms, immunofluorescence, western blotting, wound healing protocols, molecular modeling programs, and MTT assays, we comparatively examined the respective proteomic contents of WM115 primary (n = 3955 proteins) and WM266-4 metastatic (n = 6681 proteins) melanoma cells. It proved that WM115 and WM266-4 cells have engaged hybrid epithelial-to-mesenchymal transition/mesenchymal-to-epithelial transition states, with TGF-β controlling their motility in vitro. They are characterized by different signatures of SOX-dependent neural crest-like stemness and distinct architectures of the cytoskeleton network. Multiple signaling pathways have already been activated from the primary melanoma stage, whereas HIF1α, the major hypoxia-inducible factor, can be exclusively observed in metastatic melanoma cells. Invasion-metastasis cascade-specific sub-routines of activated Caspase-3-triggered apoptosis and LC3B-II-dependent constitutive autophagy were also unveiled. Importantly, WM115 and WM266-4 cells exhibited diverse drug response profiles, with epirubicin holding considerable promise as a beneficial drug for metastatic melanoma clinical management. It is the proteome navigation that enables systemic biomarkering and targeted drugging to open new therapeutic windows for advanced disease.

scholarly journals In Vitro Analysis : Inhibitory Effect of Phyllanthus niruri Extract Against Aeromonas salmonicida

BioScience ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 94
Author(s):  
Sefti Heza Dwinanti ◽  
Maya Anggita Savacka ◽  
Ade Dewi Sasanti
Keyword(s):  
Inhibitory Effect ◽  
Aeromonas Salmonicida ◽  
Phyllanthus Niruri ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Determination of microbiological and sensory parameters of fish fillets with propolis preserved under refrigeration

2014 ◽  
pp. 4214-4225 ◽  
Author(s):  
Héctor Suarez M ◽  
Álvaro Jiménez T ◽  
Consuelo Díaz M
Keyword(s):  
Storage Period ◽  
Inhibitory Effect ◽  
Fecal Coliforms ◽  
Fish Fillet ◽  
Liquid Smoke ◽  
Ethanol Extracts ◽  
Sensory Parameters ◽  
Vitro Analysis ◽  
In Vitro Analysis

ABSTRACTObjective: To determine the ability of propolis preservative cachama fillets during refrigerated storage. Materials and method. Ethanol extracts of propolis (EEP) were used in cachama fillets. The treatments included: i) 96% ethanol alcohol as the control; ii) 0.8% EEP; iii) 1.2% EEP; and iv) liquid smoke. A in vitro analysis was used to determine the inhibitory effect of propolis on Staphylococcus aureus, Escherichia coli, Salmonella sp. and Clostridium sp. and on the fish matrix to determine the mesophiles, psychrotrophiles, total coliforms, fecal coliforms, sulphite reducing spores and the presence of Salmonella. Results. The results of the in vitro analysis demonstrated the control that the EEP had over the evaluated microorganisms without presenting significant differences between the different concentrations (p>0.05). The analyses of the fish fillet matrix presented acceptable contents for the evaluated microorganisms in the treatments with EEP. A different situation was seen in the treatment with liquid smoke and the control, which had samples that where rejected after 20 days of storage. The sensory analysis showed acceptance for the samples with EEP until the end of the storage period but low marks for the treatment with liquid smoke and the control. Conclusions. The EEP used in this study could be effective for the control of Gram positive bacteria and some Gram negative bacteria that are present in cachama fillets; and could be an alternative to the use of chemical preservatives.

scholarly journals Integrin alpha 2 beta 1 is upregulated in fibroblasts and highly aggressive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils.

1991 ◽  
Vol 115 (5) ◽  
pp. 1427-1436 ◽  
Author(s):  
C E Klein ◽  
D Dressel ◽  
T Steinmayer ◽  
C Mauch ◽  
B Eckes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Cell Lines ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Collagen I ◽  
Collagen Gels ◽  
Melanoma Cell Lines

The ability of cultured human fibroblasts to reorganize and contract three dimensional collagen I gels is regarded as an in vitro model for the reorganization of connective tissue during wound healing. We investigated whether adhesion receptors of the integrin family are involved. It was found that synthesis and transcription of the alpha 2 beta 1 integrin (but not of alpha 1 beta 1 or alpha 3 beta 1) is selectively upregulated when fibroblasts are seeded into type I collagen gels. Time course experiments revealed that high synthetic levels of alpha 2 beta 1 parallel the gel contraction process and return to "baseline" levels after the contraction has subsided. Furthermore, function-blocking mAbs directed to the alpha 2 and beta 1 chain of integrins inhibited gel contraction. Remodelling of connective tissue can be important for tumor cells during invasion and formation of metastases. Therefore, we tested human melanoma cell lines for this function. Five out of nine melanoma lines contracted collagen gels in vitro. Among these, two highly aggressive melanoma cell lines (MV3 and BLM) most efficiently contracted gels almost reaching the rate of normal adult fibroblasts. In these cells, synthesis of alpha 2 beta 1 was also significantly upregulated when seeded into collagen I gels. Moreover, function blocking anti-alpha 2 in conjunction with anti-beta 1 chain mAbs completely inhibited gel contraction for several days. Other melanoma cells (530) with lower metastatic potential which were not able to contract gels, showed no induction of alpha 2 beta 1 synthesis in gel culture. Our results suggest an important role of integrin alpha 2 beta 1 in the contraction of collagen I by normal diploid fibroblasts during wound healing and in the reorganization of collagen matrices by highly aggressive human melanoma cells.

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