scholarly journals Gene Expression Profiling of Contralateral Dorsal Root Ganglia Associated with Mirror-Image Pain in a Rat Model of Complex Regional Pain Syndrome Type-I

2021 ◽  
Vol Volume 14 ◽  
pp. 2739-2756
Author(s):  
Huimin Nie ◽  
Boyu Liu ◽  
Chengyu Yin ◽  
Ruixiang Chen ◽  
Jie Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Dorsal Root Ganglia ◽  
Rat Model ◽  
Expression Profiling ◽  
Dorsal Root ◽  
Pain Syndrome ◽  
Mirror Image ◽  
Type I ◽  
Syndrome Type

scholarly journals Expression Profiling of Contralateral Dorsal Root Ganglia of a Rat Model of Complex Regional Pain Syndrome Type-i Uncovers Potential Mechanisms Involved in Mirror-image Painexpression Profiling of Contralateral Dorsal Root Ganglia of a Rat Model of Complex Regional Pain Syndrome Type-i Uncovers Potential Mechanisms Involved in Mirror-image Pain

2021 ◽  
Author(s):  
Huimin Nie ◽  
Boyu Liu ◽  
Chengyu Yin ◽  
Ruixiang Chen ◽  
Jie Wang ◽  
...  
Keyword(s):  
Complex Regional Pain Syndrome ◽  
Dorsal Root Ganglia ◽  
Expression Profiling ◽  
Dorsal Root ◽  
Pain Syndrome ◽  
Mirror Image ◽  
Type I ◽  
Syndrome Type ◽  
Rna Seq ◽  
Pain Mechanisms

Abstract Background: Mirror-image pain (MIP), which develops from the healthy body region contralateral to the actual injured site, is a mysterious pain phenomenon accompanying many chronic pain conditions, including complex regional pain syndrome (CRPS). However, the pathogenesis of MIP still remained largely unknown. The purpose of this study is to perform an expression profiling to identify genes related with MIP in an animal model of CRPS-I. Methods: We established a rat chronic post-ischemic pain (CPIP) model to mimic human CRPS-I. RNA-sequencing (RNA-Seq), bioinformatics, qPCR, immunostaining and animal behavioral assays were used to screen potential genes in contralateral dorsal root ganglia (DRG) that may be involved in MIP. Results: The CPIP model rats developed robust and persistent MIP in contralateral hind paws. Bilateral DRG neurons did not exhibit obvious neuronal damage. RNA-Seq of contralateral DRG from CPIP model rats identified a total 527 differentially expressed genes (DEGs) vs. control rats. The expression changes of several representative DEGs were verified by qPCR. Bioinformatics analysis indicated that immune system process, innate immune response and cell adhesion were among the mostly enriched biological processes, which are all important processes involved in pain sensitization, neuroinflammation and chronic pain. We further identified DEGs potentially involved in pain mechanisms or enriched in small- to medium-sized sensory neurons or TRPV1-lineage nociceptors. By comparing with published datasets summarizing genes enriched in pain mechanisms, we sorted out a core set of genes which might contribute to nociception and pain mechanism in MIP. Conclusions: We provided by far the first study to profile gene expression changes and pathway analysis of contralateral DRG for investigating MIP mechanisms. This work may provide novel insights into understanding the mysterious mechanisms underlying MIP.

Global gene expression profiling of renal scarring in a rat model of pyelonephritis

2008 ◽  
Vol 23 (7) ◽  
pp. 1059-1071 ◽  
Author(s):  
Manabu Ichino ◽  
Terumi Mori ◽  
Mamoru Kusaka ◽  
Yoko Kuroyanagi ◽  
Kiyohito Ishikawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Rat Model ◽  
Expression Profiling ◽  
Renal Scarring ◽  
Global Gene Expression ◽  
Global Gene Expression Profiling

scholarly journals Effects of Dipsacus asperoides Extract on Monosodium Iodoacetate–Induced Osteoarthritis in Rats Based on Gene Expression Profiling

2021 ◽  
Vol 12 ◽  
Author(s):  
Jin Mi Chun ◽  
A Yeong Lee ◽  
Jae Yong Nam ◽  
Kyung Seob Lim ◽  
Mu Seog Choe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protective Effect ◽  
Gene Expression Profiling ◽  
Rat Model ◽  
Expression Profiling ◽  
Bone Fractures ◽  
Cartilage Tissue ◽  
Histopathological Analysis ◽  
Therapeutic Effects ◽  
Monosodium Iodoacetate

The root of Dipsacus asperoides C. Y. Cheng et T. M. Ai is traditionally used as an analgesic and anti-inflammatory agent to treat pain, rheumatoid arthritis, and bone fractures. However, neither its effects on osteoarthritis (OA) nor its effects on the arthritic cartilage tissue transcriptome have not been fully investigated. In this study, we used a rat model of monosodium iodoacetate- (MIA-) induced OA to investigate the therapeutic effects of a Dipsacus asperoides ethanolic extract (DAE, 200 mg/kg for 21 days). The study first assessed joint diameter, micro-CT scans, and histopathological analysis and then conducted gene expression profiling using RNA sequencing in articular cartilage tissue. We found that DAE treatment ameliorates OA disease phenotypes; it reduced the knee joint diameter and prevented changes in the structural and histological features of the joint, thereby showing that DAE has a protective effect against OA. Based on the results of gene expression profiling and subsequent pathway analysis, we found that several canonical pathways were linked to DAE treatment, including WNT/β-catenin signaling. Taken together, the present results suggest molecular mechanism, involving gene expression changes, by which DAE has a protective effect in a rat model of MIA-induced OA.

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