scholarly journals Activating transcription factor 3 modulates protein kinase C epsilon activation in diabetic peripheral neuropathy

2019 ◽  
Vol Volume 12 ◽  
pp. 317-326
Author(s):  
Ying-Shuang Chang ◽  
Hung-Wei Kan ◽  
Yu-Lin Hsieh
Keyword(s):  
Transcription Factor ◽  
Protein Kinase C ◽  
Peripheral Neuropathy ◽  
Protein Kinase ◽  
Diabetic Peripheral Neuropathy ◽  
Activating Transcription Factor 3 ◽  
Kinase C ◽  
Protein Kinase C Epsilon ◽  
Activating Transcription Factor ◽  
Transcription Factor 3

scholarly journals Genetic loss-of-function of activating transcription factor 3 but not C-type lectin member 5A prevents diabetic peripheral neuropathy

2021 ◽  
Author(s):  
Hung-Wei Kan ◽  
Chin-Hong Chang ◽  
Ying-Shuang Chang ◽  
Yi-Ting Ko ◽  
Yu-Lin Hsieh
Keyword(s):  
Transcription Factor ◽  
Peripheral Neuropathy ◽  
Er Stress ◽  
Diabetic Peripheral Neuropathy ◽  
Protein Translation ◽  
Initiation Factor ◽  
Heme Oxygenase 1 ◽  
Activating Transcription Factor 3 ◽  
Activating Transcription Factor ◽  
Transcription Factor 3

AbstractWe investigated the mediating roles of activating transcription factor 3 (ATF3), an injury marker, or C-type lectin member 5A (CLEC5A), an inflammatory response molecule, in the induction of endoplasmic reticulum (ER) stress and neuroinflammation in diabetic peripheral neuropathy in ATF3 and CLEC5A genetic knockout (aft3−/− and clec5a−/−, respectively) mice. ATF3 was expressed intranuclearly and was upregulated in mice with diabetic peripheral neuropathy (DN) and clec5a−/− mice. The DN and clec5a−/− groups also exhibited neuropathic behavior, but not in the aft3−/− group. The upregulation profiles of cytoplasmic polyadenylation element-binding protein, a protein translation–regulating molecule, and the ER stress-related molecules of inositol-requiring enzyme 1α and phosphorylated eukaryotic initiation factor 2α in the DN and clec5a−/− groups were correlated with neuropathic behavior. Ultrastructural evidence confirmed ER stress induction and neuroinflammation, including microglial enlargement and proinflammatory cytokine release, in the DN and clec5a−/− mice. By contrast, the induction of ER stress and neuroinflammation did not occur in the aft3−/− mice. Furthermore, the mRNA of reactive oxygen species–removing enzymes such as superoxide dismutase, heme oxygenase-1, and catalase were downregulated in the DN and clec5a−/− groups but were not changed in the aft3−/− group. Taken together, the results indicate that intraneuronal ATF3, but not CLEC5A, mediates the induction of ER stress and neuroinflammation associated with diabetic neuropathy.

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