scholarly journals Traumatic osteoarthritis-induced persistent mechanical hyperalgesia in a rat model of anterior cruciate ligament transection plus a medial meniscectomy

2017 ◽  
Vol Volume 11 ◽  
pp. 41-50 ◽  
Author(s):  
Hsiao-Chien Tsai ◽  
Ta-Liang Chen ◽  
Yu-Pin Chen ◽  
Ruei-Ming Chen
Keyword(s):  
Anterior Cruciate Ligament ◽  
Rat Model ◽  
Cruciate Ligament ◽  
Mechanical Hyperalgesia ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate

scholarly journals Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats

2018 ◽  
Author(s):  
Sabri Sudirman ◽  
Alan Darmasaputra Ong ◽  
Heng-Wei Chang ◽  
Zwe-Ling Kong
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cholesterol Level ◽  
High Fat Diet ◽  
Cruciate Ligament ◽  
Weight Bearing ◽  
Hdl Cholesterol ◽  
High Fat ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate

Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in female. Obesity and mechanical injury causing OA are attributed to joint loading, cartilage disintegration, bone loss and inflammation as well. Several strategies used for treatment OA including non-pharmacological and pharmacological. Fucoidan possesses several bioactivities such as antitumor, antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect of fucoidan in surgery-induced OA on diet-induced obesity rats. OA was induced by anterior cruciate ligament transection and partial medial meniscectomy (ACLT+MMx). Male SD rats were fed high-fat diet (HFD) for 4 weeks to induce obesity before ACLT+MMx to induce OA. OA rats were administered with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) after the surgeries for 40 days with HFD. We observed that the swelling in knee joint was alleviated and hind paw weight distribution was rectified after feeding fucoidan, with no significant effect on weight gain and feed intake. Fucoidan administration indicated no significant variation on HDL-Cholesterol level, but reduced plasma triglycerides and LDL-Cholesterol level. In addition, weight-bearing tests showed improvement in the fucoidan-treated group. Our results suggested that fucoidan may improve meniscal/ligamentous injury and obesity-induced OA.

scholarly journals Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats

2018 ◽  
Author(s):  
Sabri Sudirman ◽  
Alan Darmasaputra Ong ◽  
Heng-Wei Chang ◽  
Zwe-Ling Kong
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cholesterol Level ◽  
High Fat Diet ◽  
Cruciate Ligament ◽  
Weight Bearing ◽  
Hdl Cholesterol ◽  
High Fat ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate

Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in female. Obesity and mechanical injury causing OA are attributed to joint loading, cartilage disintegration, bone loss and inflammation as well. Several strategies used for treatment OA including non-pharmacological and pharmacological. Fucoidan possesses several bioactivities such as antitumor, antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect of fucoidan in surgery-induced OA on diet-induced obesity rats. OA was induced by anterior cruciate ligament transection and partial medial meniscectomy (ACLT+MMx). Male SD rats were fed high-fat diet (HFD) for 4 weeks to induce obesity before ACLT+MMx to induce OA. OA rats were administered with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) after the surgeries for 40 days with HFD. We observed that the swelling in knee joint was alleviated and hind paw weight distribution was rectified after feeding fucoidan, with no significant effect on weight gain and feed intake. Fucoidan administration indicated no significant variation on HDL-Cholesterol level, but reduced plasma triglycerides and LDL-Cholesterol level. In addition, weight-bearing tests showed improvement in the fucoidan-treated group. Our results suggested that fucoidan may improve meniscal/ligamentous injury and obesity-induced OA.

miR-29b is Involved in Cartilage Autophagy and Muscle Atrophy in a Rat Model of Knee Osteoarthritis

2020 ◽  
Author(s):  
Che Ji ◽  
Jia Peiyu ◽  
Ma Yantao ◽  
Han Qi ◽  
Wang Xiaolei ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
Knee Osteoarthritis ◽  
Therapeutic Effect ◽  
Muscle Atrophy ◽  
Rat Model ◽  
Cruciate Ligament ◽  
Weight Bearing ◽  
Periodic Acid ◽  
Anterior Cruciate Ligament Transection ◽  
Anterior Cruciate

Abstract Background Knee osteoarthritis (KOA) is a progressively degenerative form of arthritis characterized by chondrocyte apoptosis and cartilage degeneration. KOA also involves limb muscle atrophy, especially in the quadriceps muscles. However, there are limited options for the treatment of KOA. miR-29b stimulates apoptosis in the chondrocytes from patients with KOA and muscle atrophy in other models. Therefore, we investigated the therapeutic effect of miR-29b in cartilage autophagy and muscle atrophy. Methods Ten rats comprised the control cohort without anterior cruciate ligament transection. The treatment group (KOA induced in the right knee via anterior cruciate ligament transection) was divided into a model untreated group and a miR-29b-antagomir group (miR-29b antagomir injected 1 wk before surgery). Results Real-time polymerase chain reaction revealed successful downregulation of miR-29b using antagomir in the joints and muscles. A weight-bearing test showed that miR-29b downregulation affected joint function. Enzyme-linked immunosorbent assays demonstrated that downregulating miR-29b reduced pro-inflammatory cytokine expression. Immunohistochemistry revealed that miR-29b depletion enhanced autophagy by activating LC3 and beclin-1 in the cartilage. Autophagy was stimulated by the activation of MAPK and mTOR signaling. Depletion of miR-29b ameliorated the decrease in the weight of the quadriceps and the quadriceps weight/body weight ratio of the rats. Hematoxylin–eosin and periodic acid–Schiff staining showed that miR-29b downregulation inhibited muscular atrophy. Immunofluorescence showed that miR-29b downregulation affected IGF/PI3K/AKT signaling. Conclusions This study demonstrated the therapeutic effect of miR-29b on autophagy in the cartilage and on muscle atrophy in a rat model for KOA, highlighting the potential of miR-29b as a therapeutic target for KOA.

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