scholarly journals TRPA1 in the spinal dorsal horn is involved in post-inflammatory visceral hypersensitivity: in vivo study using TNBS-treated rat model

2016 ◽  
Vol Volume 9 ◽  
pp. 1153-1160 ◽  
Author(s):  
Qian Li ◽  
Chenghao Guo ◽  
Mohammed Ali Chowdhury ◽  
Taoli Dai ◽  
Wei Han
Keyword(s):  
Dorsal Horn ◽  
Rat Model ◽  
Spinal Dorsal Horn ◽  
Visceral Hypersensitivity ◽  
In Vivo Study ◽  
Spinal Dorsal

scholarly journals Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Zhenyu Wu ◽  
Xiaofang Lu ◽  
Shengsheng Zhang ◽  
Chunyang Zhu
Keyword(s):  
Gene Expression ◽  
Dorsal Horn ◽  
Functional Dyspepsia ◽  
Rat Model ◽  
Spinal Dorsal Horn ◽  
Visceral Hypersensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gastric Distention ◽  
Real Time Quantitative Pcr ◽  
Spinal Dorsal

The present study investigated the effect of Chinese medicine Sini-San (SNS) on visceral hypersensitivity in a rat model of functional dyspepsia (FD), and it explored related underlying mechanisms. The rat model of FD was developed by combining neonatal iodoacetamide (IA) treatment and adult tail-clamping. After SNS treatment, the behavior and electromyographic testing were performed to evaluate the visceromotor responses of rats to gastric distention. Immunofluorescence was used to detect the distribution of iNOS-positive cells in the spinal dorsal horn, while the real-time quantitative PCR and western blot were used for detection of the gene expression of c-fos, iNOS, and GABAb and protein levels of iNOS and GABAb in the spinal dorsal horn, respectively. The protein concentration of cGMP and PKG proteins in the spinal dorsal horn were quantified by enzyme-linked immunosorbent assay. In this study, SNS treatment significantly reduced the behavioral score and electromyographic response to graded intragastric distension pressure. The middle-dose of SNS treatment significantly reduced the distribution of iNOS-positive cells in the spinal dorsal horn of FD model rats. The gene expression of c-fos, iNOS, and GABAb and the protein contents of iNOS, GABAb, cGMP, and PKG in the spinal dorsal horn of FD model rats were restored to a normal level by middle-dose of SNS treatment. Our results suggest that Sini-San may alleviate the visceral hypersensitivity in FD model rats via regulation of the NO/cGMP/PKG pathway in the spinal dorsal horn.

306 IN VIVO IMAGING OF Ca 2+ GRADIENTS IN SPINAL DORSAL HORN NEURONS

2009 ◽  
Vol 13 (S1) ◽  
Author(s):  
M. Gassner ◽  
M. Wagner ◽  
H. Fischer ◽  
R. Drdla ◽  
T. Jäger ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
In Vivo Imaging ◽  
Spinal Dorsal Horn ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Spinal Dorsal Horn Neurons

Measurement of cholecystokinin release in vivo in the rat spinal dorsal horn

1999 ◽  
Vol 4 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Henrik Gustafsson ◽  
Guilherme de Araujo Lucas ◽  
Eva Schött ◽  
Carl-Olav Stiller ◽  
Pawel Alster ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Spinal Dorsal

scholarly journals Evaluation of Formalin-Induced Pain Behavior and Glutamate Release in the Spinal Dorsal Horn Using In Vivo Microdialysis in Conscious Rats

2012 ◽  
Vol 120 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Alba Vidal-Torres ◽  
Alicia Carceller ◽  
Daniel Zamanillo ◽  
Manuel Merlos ◽  
Jos^|^eacute; Miguel Vela ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Glutamate Release ◽  
In Vivo Microdialysis ◽  
Pain Behavior ◽  
Conscious Rats ◽  
Spinal Dorsal

scholarly journals Inhibitory action of nociceptin on spinal dorsal horn neurones of the rat, in vivo

1996 ◽  
Vol 118 (8) ◽  
pp. 1875-1877 ◽  
Author(s):  
L.C. Stanfa ◽  
V. Chapman ◽  
N. Kerr ◽  
A.H. Dickenson
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Inhibitory Action ◽  
Spinal Dorsal ◽  
Dorsal Horn Neurones

scholarly journals Noradrenaline modulates mechanically evoked responses in the rat spinal dorsal horn: an in vivo patch-clamp study

2019 ◽  
Vol Volume 12 ◽  
pp. 1269-1278 ◽  
Author(s):  
Motoki Sonohata ◽  
Atsushi Doi ◽  
Toshiharu Yasaka ◽  
Daisuke Uta ◽  
Masaaki Mawatari ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Evoked Responses ◽  
Spinal Dorsal ◽  
Clamp Study

In Vivo Patch-Clamp Analysis of IPSCs Evoked in Rat Substantia Gelatinosa Neurons by Cutaneous Mechanical Stimulation

2000 ◽  
Vol 84 (4) ◽  
pp. 2171-2174 ◽  
Author(s):  
Keita Narikawa ◽  
Hidemasa Furue ◽  
Eiichi Kumamoto ◽  
Megumu Yoshimura
Keyword(s):  
Patch Clamp ◽  
Receptor Antagonist ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Substantia Gelatinosa ◽  
Mechanical Stimuli ◽  
Patch Clamp Technique ◽  
Spinal Dorsal ◽  
Postsynaptic Currents

To know a functional role of inhibitory synaptic responses in transmitting noxious and innoxious information from the periphery to the rat spinal dorsal horn, we examined inhibitory postsynaptic currents (IPSCs) elicited in substantia gelatinosa (SG) neurons by mechanical stimuli applied to the skin using the newly developed in vivo patch-clamp technique. In the majority (80%) of SG neurons examined, a brush stimulus applied to the ipsilateral hind limb produced a barrage of IPSCs that persisted during the stimulus, while a pinch stimulus evoked IPSCs only at its beginning and end. The pinch-evoked IPSCs may have been caused by a touch that occurs at the on/off time of the pinch. The evoked IPSCs were blocked by either a glycine-receptor antagonist, strychnine (4 μM), or a GABAA-receptor antagonist, bicuculline (20 μM). All SG neurons examined received inhibitory inputs from a wide area throughout the thigh and lower leg. When IPSCs were examined together with excitatory postsynaptic currents (EPSCs) in the same neurons, a brush evoked a persistent activity of both IPSCs and EPSCs during the stimulus while a pinch evoked such an activity of EPSCs but not IPSCs. It is suggested that innoxious mechanical stimuli activate a GABAergic or glycinergic circuitry in the spinal dorsal horn. This inhibitory transmission may play an important role in the modulation of noxious information in the SG.

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