scholarly journals In vitro expression of NLRP inflammasome-induced active Caspase-1 expression in normal human epidermal keratinocytes (NHEK) by various exogenous threats and subsequent inhibition by naturally derived ingredient blends

2019 ◽  
Vol Volume 12 ◽  
pp. 219-230 ◽  
Author(s):  
James V Gruber ◽  
Robert Holtz
Keyword(s):  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
In Vitro Expression ◽  
Caspase 1 ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human ◽  
Active Caspase

scholarly journals High Affinity of Hybrid Liposomes for Normal Human Epidermal Keratinocytes in Vitro.

1994 ◽  
Vol 17 (9) ◽  
pp. 1299-1300 ◽  
Author(s):  
Yoko MATSUMOTO ◽  
Eiichi YAMADA ◽  
Shoji KAMEI ◽  
Masayoshi IWAHARA ◽  
Ryuichi UEOKA
Keyword(s):  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
High Affinity ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human

TRANSFECTION OF NORMAL HUMAN EPIDERMAL KERATINOCYTES WITH LIPID/DNA COMPLEXES IN VITRO

2001 ◽  
Vol 11 (1) ◽  
pp. 43-54
Author(s):  
Sebastian Zellmer ◽  
Andrej Surovoy ◽  
Frank Gaunitz ◽  
Jürgen Salvetter ◽  
Rolf Gebhardt ◽  
...  
Keyword(s):  
Epidermal Keratinocytes ◽  
Dna Complexes ◽  
Human Epidermal Keratinocytes ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human

scholarly journals Asiaticoside increases aquaporin-3 protein expression in the cytoplasm of normal human epidermal keratinocytes

2017 ◽  
Vol 36 (1) ◽  
pp. 25
Author(s):  
Linda Yulianti Wijayadi ◽  
Hari Darmawan
Keyword(s):  
Active Agent ◽  
Skin Hydration ◽  
Epidermal Keratinocytes ◽  
Control Group ◽  
Major Protein ◽  
Human Epidermal Keratinocytes ◽  
Aquaporin 3 ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human

<p>Introduction<br />Skin hydration decreases with aging. Aquaporin-3 (AQP3) is a major protein that plays a role in skin hydration, therefore it is a novel target for skin moisturizing treatment. Retinoic acid (RA) as a well-known active agent in antiaging treatment increases AQP3 expression, but frequently causes harmful side effects. Asiaticoside, a saponin compound isolated from Centella asiatica (CA) is also known as an antiaging cosmetic and plays a role in wound healing. The aim of this study was to evaluate and compare the effect of asiaticoside isolated from CA and the effect of RA on the AQP3 expression in normal human epidermal keratinocytes (NHEKs).</p><p>Methods <br />An experimental laboratory study was performed using primary NHEKs that were derived from the foreskin of a boy. AQP3 expression in NHEKs was examined in vitro after the cells were incubated for 24 hours with asiaticoside or with RA at several concentrations. The AQP3 expression was evaluated by immunocytochemistry and quantitatively analyzed by Image-J software. Independent t-test and one-way ANOVA were used to analyze the data, followed by post-hoc Tukey test.</p><p>Results<br />There was an increasing trend of AQP3 expression upon exposure to asiaticoside at all concentrations compared to the control group. However, RA exposure seemed to induce a higher level of AQP3 expression. Asiaticoside effected a lower increase in AQP3 expression in NHEKs than did RA (p=0.042). Optimal results were achieved at 1 mg/mL concentration of asiaticoside.</p><p>Conclusions<br />Asiaticoside isolated from CA can enhance the AQP3 expression in NHEKs. Therefore it can be used as an active ingredient in cosmetic moisturizer formulation for dry skin treatment.</p>

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