scholarly journals Bactericidal effect of iron oxide nanoparticles on Staphylococcus aureus

2010 ◽  
pp. 277 ◽  
Author(s):  
Thomas J Thomas Webster
Keyword(s):  
Staphylococcus Aureus ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Bactericidal Effect ◽  
Oxide Nanoparticles

scholarly journals Reduced Staphylococcus aureus biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

2016 ◽  
Vol Volume 11 ◽  
pp. 6499-6506 ◽  
Author(s):  
Sifeng Shi ◽  
Jingfu Jia ◽  
XiaoKui Guo ◽  
Yaping Zhao ◽  
Desheng Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biofilm Formation ◽  
Oxide Nanoparticles

scholarly journals Effects of iron nano-particle’s on expression of tetracycline resistance encoding genes in Staphylococcus aureus by Real Time-PCR

2018 ◽  
Vol 69 (2) ◽  
pp. 973
Author(s):  
H. ARAB ◽  
B. SHOJAEE SADI ◽  
K. AMINI
Keyword(s):  
Staphylococcus Aureus ◽  
Iron Oxide ◽  
Real Time ◽  
Iron Oxide Nanoparticles ◽  
Real Time Pcr ◽  
Antibiotic Resistance Gene ◽  
Health Concern ◽  
Aureus Strain ◽  
Oxide Nanoparticles ◽  
Antibiotic Susceptibility Test

Increasing bacterial resistance towards traditional/conventional antibiotics is a major global health concern worldwide. Iron oxide nanoparticles (Fe nanoparticles, with average size of 20 nm) have considerable potential as antimicrobial agents in food safety applications due to their structure, surface properties, and stability. The aim of this work was to investigate the antibacterial effects and mechanism of action of iron nanoparticles against the expression of the tetA gene in Tetracycline Resistant Staphylococcus aureus strains by real time PCR. In the cross-sectional study, a total of 60 S. aureus were collected. Antibiotic susceptibility test was performed on the muller hinton agar according to the Clinical and Laboratory Standards Institute (CLSI). Then all strains were evaluated for tetA, tetB, tetC and tetD genes by multiplex-PCR method. In-vitro activity of iron oxide nanoparticles was evaluated against all resistant strains by microbroth dilution method. Therefore, the expression of tetA gene was measured in treated with iron oxide nanoparticles and untreated resistant S. aureus strain by Real time PCR. Our results indicated 25 (41.66%) strains resistant to Tetracycline. The prevalence of tetA, tetB, tetC and tetD genes were 5 (8.33%), 2 (2.33%), 20 (33.33%) and 10 (10.67%), respectively. The expression of tetA genes in resistant S. aureus strains treated with Iron oxide nanoparticles was lower than the untreated isolates. Iron oxide nanoparticles have strong antibacterial activity against resistant to Tetracycline S. aureus strains. In addition to, these nanoparticles reduce the expression of antibiotic resistance gene.

scholarly journals Green synthesis of Iron oxide nanoparticles using Lagenaria siceraria and evaluation of its Antimicrobial activity

2017 ◽  
Vol 2 (4) ◽  
pp. 422 ◽  
Author(s):  
S. Kanagasubbulakshmi ◽  
K. Kadirvelu
Keyword(s):  
Staphylococcus Aureus ◽  
Iron Oxide ◽  
Green Synthesis ◽  
Iron Oxide Nanoparticles ◽  
Antimicrobial Property ◽  
Oxide Nanoparticles ◽  
Main Role ◽  
Lagenaria Siceraria ◽  
Escherchia Coli ◽  
Ft Ir

<p class="p1">Magnetic iron oxide nanoparticles (MNPs) with appropriate surface chemistry exhibit many interesting properties that can be exploited in a variety of biomedical applications such as magnetic resonance imaging contrast enhancement, tissue repair, hyperthermia, drug delivery and in cell separation. In this study unexplored <em>Lagenaria siceraria </em>leaves extract was found to be capable in green synthesis of Iron oxide nanoparticles (Fe<span class="s2">3</span>O<span class="s2">4</span>-NPs) and their characteristics were studied by using UV-visible spectrophotometer, SEM, EDX, XRD, Zeta sizer and FT-IR. Thus synthesized Fe<span class="s2">3</span>O<span class="s2">4</span>-NPs were naturally stabilized, cubic shaped and in the size range of 30-100 nm. The phytochemicals present in the leaf has a main role as reducing agent that assists to the eco friendly synthesis of Fe<span class="s2">3</span>O<span class="s2">4</span>-NPs with enhanced antioxidant property. Functional groups present on the NPs are mainly –OH and –COOH (FT-IR) makes it hydrophilic hence NPs does not need any further functional modification for applications. The antimicrobial property of synthesized Fe<span class="s2">3</span>O<span class="s2">4</span>-NPs was evaluated against Gram negative - <em>Escherchia coli, </em>Gram positive- <em>Staphylococcus aureus, </em>The Zone of inhibition was found to be 10 mm for <em>Escherchia coli, </em>and 8 mm for <em>Staphylococcus aureus. </em>Thus naturally stabilized Fe<span class="s2">3</span>O<span class="s2">4</span>-NPs with herbal property can be used in various biological applications.</p>

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Sign in / Sign up

Export Citation Format

Share Document