scholarly journals Cationic polyelectrolyte-mediated delivery of antisense morpholino oligonucleotides for exon-skipping in vitro and in mdx mice

2015 ◽  
pp. 5635 ◽  
Author(s):  
Mingxing Wang ◽  
Bo Wu ◽  
Jason D. Tucker ◽  
Peijuan Lu ◽  
Qilong Lu
Keyword(s):  
Exon Skipping ◽  
Cationic Polyelectrolyte ◽  
Mdx Mice ◽  
Morpholino Oligonucleotides ◽  
Antisense Morpholino Oligonucleotides ◽  
Antisense Morpholino

scholarly journals U1 snRNP regulates cancer cell migration and invasion

2019 ◽  
Author(s):  
Jung-Min Oh ◽  
Christopher C. Venters ◽  
Chao Di ◽  
Anna Maria Pinto ◽  
Lili Wan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Migration And Invasion ◽  
Small Nuclear Rna ◽  
Mrna Isoforms ◽  
Over Expression ◽  
Morpholino Oligonucleotides ◽  
Antisense Morpholino Oligonucleotides ◽  
Antisense Morpholino ◽  
Polyadenylation Signals

AbstractStimulated cells and cancer cells have widespread shortening of mRNA 3’-utranslated regions (3’UTRs) and switches to shorter mRNA isoforms due to usage of more proximal polyadenylation signals (PASs) in the last exon and in introns. U1 snRNA (U1), vertebrates’ most abundant non-coding (spliceosomal) small nuclear RNA, silences proximal PASs and its inhibition with antisense morpholino oligonucleotides (U1 AMO) triggers widespread mRNA shortening. Here we show that U1 AMO also modulates cancer cells’ phenotype, dose-dependently increasing migration and invasion in vitro by up to 500%, whereas U1 over-expression has the opposite effect. In addition to 3’UTR length, numerous transcriptome changes that could contribute to this phenotype are observed, including alternative splicing, and mRNA expression levels of proto-oncogenes and tumor suppressors. These findings reveal an unexpected link between U1 regulation and oncogenic and activated cell states, and suggest U1 as a potential target for their modulation.

scholarly journals U1 snRNP regulates cancer cell migration and invasion in vitro

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jung-Min Oh ◽  
Christopher C. Venters ◽  
Chao Di ◽  
Anna Maria Pinto ◽  
Lili Wan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Migration And Invasion ◽  
Small Nuclear Rna ◽  
Mrna Isoforms ◽  
Over Expression ◽  
U1 Snrnp ◽  
Morpholino Oligonucleotides ◽  
Antisense Morpholino Oligonucleotides ◽  
Antisense Morpholino

AbstractStimulated cells and cancer cells have widespread shortening of mRNA 3’-untranslated regions (3’UTRs) and switches to shorter mRNA isoforms due to usage of more proximal polyadenylation signals (PASs) in introns and last exons. U1 snRNP (U1), vertebrates’ most abundant non-coding (spliceosomal) small nuclear RNA, silences proximal PASs and its inhibition with antisense morpholino oligonucleotides (U1 AMO) triggers widespread premature transcription termination and mRNA shortening. Here we show that low U1 AMO doses increase cancer cells’ migration and invasion in vitro by up to 500%, whereas U1 over-expression has the opposite effect. In addition to 3’UTR length, numerous transcriptome changes that could contribute to this phenotype are observed, including alternative splicing, and mRNA expression levels of proto-oncogenes and tumor suppressors. These findings reveal an unexpected role for U1 homeostasis (available U1 relative to transcription) in oncogenic and activated cell states, and suggest U1 as a potential target for their modulation.

scholarly journals Polyquaternium-mediated delivery of morpholino oligonucleotides for exon-skipping in vitro and in mdx mice

Drug Delivery ◽  
2017 ◽  
Vol 24 (1) ◽  
pp. 952-961 ◽  
Author(s):  
Mingxing Wang ◽  
Bo Wu ◽  
Sapana N Shah ◽  
Peijuan Lu ◽  
Qilong Lu
Keyword(s):  
Exon Skipping ◽  
Mdx Mice ◽  
Morpholino Oligonucleotides

scholarly journals Tween 85-Modified Low Molecular Weight PEI Enhances Exon-Skipping of Antisense Morpholino Oligomer In Vitro and in mdx Mice

2017 ◽  
Vol 9 ◽  
pp. 120-131 ◽  
Author(s):  
Mingxing Wang ◽  
Bo Wu ◽  
Jason D. Tucker ◽  
Sapana N. Shah ◽  
Peijuan Lu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Exon Skipping ◽  
Mdx Mice ◽  
Low Molecular Weight ◽  
Antisense Morpholino

In vitro Correction of a Novel Splicing Alteration in the BTK Gene by Using Antisense Morpholino Oligonucleotides

2014 ◽  
Vol 62 (5) ◽  
pp. 431-436 ◽  
Author(s):  
Natthakorn Rattanachartnarong ◽  
Siraprapa Tongkobpetch ◽  
Pantipa Chatchatee ◽  
Tassalapa Daengsuwan ◽  
Chupong Ittiwut ◽  
...  
Keyword(s):  
Morpholino Oligonucleotides ◽  
Antisense Morpholino Oligonucleotides ◽  
Antisense Morpholino

Tween 85 grafted PEIs enhanced delivery of antisense 2′-O-methyl phosphorothioate oligonucleotides in vitro and in dystrophic mdx mice

2015 ◽  
Vol 3 (26) ◽  
pp. 5330-5340 ◽  
Author(s):  
Mingxing Wang ◽  
Bo Wu ◽  
Jason D. Tucker ◽  
Peijuan Lu ◽  
Lauren E. Bollinger ◽  
...  
Keyword(s):  
Exon Skipping ◽  
Mdx Mice

The most effective Tween 85 modified LPEI (Z7) enhanced exon-skipping of 2′-OMePS over 8 folds compared with 2′-OMePS alone inmdxmice, without increasing toxicity.

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