scholarly journals Methotrexate-loaded lipid-core nanocapsules are highly effective in the control of inflammation in synovial cells and a chronic arthritis model

2015 ◽  
pp. 6603 ◽  
Author(s):  
Silvia Guterres ◽  
Catiúscia Oliveira ◽  
Andrea Tarrago ◽  
Allyson Guimarães ◽  
Adriana Malheiro ◽  
...  
Keyword(s):  
Chronic Arthritis ◽  
Synovial Cells ◽  
Arthritis Model ◽  
Lipid Core ◽  
Highly Effective

The effectiveness of Echinacea extract or composite glucosamine, chondroitin and methyl sulfonyl methane supplements on acute and chronic rheumatoid arthritis rat model

2011 ◽  
Vol 29 (2) ◽  
pp. 187-201 ◽  
Author(s):  
Nadia MS Arafa ◽  
Hayam M Hamuda ◽  
Samuel T Melek ◽  
Sahar K Darwish
Keyword(s):  
Rat Model ◽  
Chondroitin Sulphate ◽  
Cartilage Damage ◽  
Treated Group ◽  
Chronic Arthritis ◽  
Synovial Cells ◽  
Female Rat ◽  
Faecal Flora ◽  
Reactive Protein ◽  
Rf Values

The study aimed to investigate the effect of the oral administration for 15 days of either Echinacea (E) or genuphil (a composite of chondroitin sulphate, glucosamine and methyl sulfonyl methane [GCM]) nutraceutical supplements on female rat model of acute or chronic arthritis induced by bacterial outer membrane protein (OMP) from faecal flora of healthy and rheumatic humans. Anti-cyclic citrullinated peptide (anti-CCP2), C-reactive protein (CRP) and rheumatoid factor (RF) values increased ( p < 0.05) in both arthritic groups as compared to normal values. The rheumatic markers anti-CCP2, CRP and RF values decreased significantly in E- and GCM-treated groups compared to arthritic none-treated acute or chronic groups. The results of RF values of GCM-treated groups in acute and chronic models decreased exhibiting no statistical difference compared with the normal value. Histological examinations of the hind paw sections revealed moderate inflammation, oedema and mild proliferation of synovial cells in acute arthritic rats and more damage to cartilage and bone with severe inflammation in chronic ones. Echinacea acute treated group showed edema with proliferated synovial membrane and partial damage in cartilage and bone. While in the E -chronic treated group, rough edge with destructed cartilage and bone existed. However, the acute GCM group revealed mild cartilage damage. But the chronic GCM group showed mild synovial cells proliferation and revealed no inflammation with mild cartilage damage edge. Results demonstrated the OMP arthropathic property and through promising light on arthritis treatment using E- or GCM, with the advantage of GMC results over that of E -. The composite GCM is needed for further studies over the dose and duration to assess its preventive effects against the bacterial OMP arthrogenicity.

Lipid-Core Nanocapsules Improved Antiedematogenic Activity of Tacrolimus in Adjuvant-Induced Arthritis Model

2016 ◽  
Vol 16 (2) ◽  
pp. 1265-1274 ◽  
Author(s):  
Rossana B. Friedrich ◽  
Karine Coradini ◽  
Francisco N. Fonseca ◽  
Silvia S. Guterres ◽  
Ruy C. R. Beck ◽  
...  
Keyword(s):  
Arthritis Model ◽  
Lipid Core ◽  
Adjuvant Induced Arthritis

scholarly journals Role of 11β-HSD type 1 in abnormal HPA axis activity during immune-mediated arthritis

2018 ◽  
Vol 7 (2) ◽  
pp. 385-394 ◽  
Author(s):  
Janko Sattler ◽  
Jinwen Tu ◽  
Shihani Stoner ◽  
Jingbao Li ◽  
Frank Buttgereit ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Hpa Axis ◽  
Brain Regions ◽  
Chronic Arthritis ◽  
Arthritis Model ◽  
Acute Arthritis ◽  
Immune Mediated ◽  
The Central Nervous System ◽  
Hpa Axis Activity

Patients with chronic immune-mediated arthritis exhibit abnormal hypothalamo-pituitary-adrenal (HPA) axis activity. The basis for this abnormality is not known. Immune-mediated arthritis is associated with increased extra-adrenal synthesis of active glucocorticoids by the 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) enzyme. 11β-HSD1 is expressed in the central nervous system, including regions involved in HPA axis regulation. We examined whether altered 11β-HSD1 expression within these regions contributes to HPA axis dysregulation during arthritis. The expression of 11β-HSD1, and other components of glucocorticoid signaling, were examined in various brain regions and the pituitary gland of mice with experimentally induced arthritis. Two arthritis protocols were employed: The K/BxN spontaneous arthritis model for chronic arthritis and the K/BxN serum transfer arthritis model for acute arthritis. 11β-HSD1 mRNA (Hsd11b1) was expressed in the hippocampus, hypothalamus, cortex, cerebellum and pituitary gland. Hypothalamic Hsd11b1 expression did not change in response to arthritis in either model. Pituitary Hsd11b1 expression was however significantly increased in both chronic and acute arthritis models. Hippocampal Hsd11b1 was decreased in acute but not chronic arthritis. Chronic, but not acute, arthritis was associated with a reduction in hypothalamic corticotropin-releasing hormone and arginine vasopressin expression. In both models, serum adrenocorticotropic hormone and corticosterone levels were no different from non-inflammatory controls. These findings demonstrate inflammation-dependent regulation of Hsd11b1 expression in the pituitary gland and hippocampus. The upregulation of 11β-HSD1 expression in the pituitary during both chronic and acute arthritis, and thus, an increase in glucocorticoid negative feedback, could contribute to the abnormalities in HPA axis activity seen in immune-mediated arthritis.

scholarly journals Self-reactive T cells induce and perpetuate chronic relapsing arthritis

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Jonatan Tuncel ◽  
Jens Holmberg ◽  
Sabrina Haag ◽  
Malin Hultqvist Hopkins ◽  
Lena Wester-Rosenlöf ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
Inflammatory Responses ◽  
Chronic Arthritis ◽  
Conclusive Evidence ◽  
Whole Body ◽  
Sustained Remission ◽  
Disease Course ◽  
Arthritis Model ◽  
Relapsing Remitting

Abstract Background CD4+ T cells play a central role during the early stages of rheumatoid arthritis (RA), but to which extent they are required for the perpetuation of the disease is still not fully understood. The aim of the current study was to obtain conclusive evidence that T cells drive chronic relapsing arthritis. Methods We used the rat pristane-induced arthritis model, which accurately portrays the chronic relapsing-remitting disease course of RA, to examine the contribution of T cells to chronic arthritis. Results Rats subjected to whole-body irradiation and injected with CD4+ T cells from lymph nodes of pristane-injected donors developed chronic arthritis that lasted for more than 4 months, whereas T cells from the spleen only induced acute disease. Thymectomy in combination with irradiation enhanced the severity of arthritis, suggesting that sustained lymphopenia promotes T cell-driven chronic inflammation in this model. The ability of T cells to induce chronic arthritis correlated with their expression of Th17-associated transcripts, and while depletion of T cells in rats with chronic PIA led to transient, albeit significant, reduction in disease, neutralization of IL-17 resulted in almost complete and sustained remission. Conclusion These findings show that, once activated, self-reactive T cells can sustain inflammatory responses for extended periods of time and suggest that such responses are promoted in the presence of IL-17.

EFFECTS OF HYALURONAN ON CHRONIC ARTHRITIS MODEL IN RABBIT

Hyaluronan ◽  
2002 ◽  
pp. 401-406
Author(s):  
Sachiko Gotoh ◽  
Katsuya Takahashi ◽  
Junichi Onaya ◽  
Kazuo Okamura ◽  
Katsuyuki Horie
Keyword(s):  
Chronic Arthritis ◽  
Arthritis Model

Rapid demonstration of septic or infectious arthritis due to Gram-negative bacteria

1992 ◽  
Vol 50 (1) ◽  
pp. 686-687
Author(s):  
Jacob S. Hanker ◽  
Paul R. Gross ◽  
Beverly L. Giammara
Keyword(s):  
Chronic Arthritis ◽  
Blood Cultures ◽  
Gram Negative Bacteria ◽  
Infectious Arthritis ◽  
Bacterial Arthritis ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Blood cultures are positive in approximately only 50 per cent of the patients with nongonococcal bacterial infectious arthritis and about 20 per cent of those with gonococcal arthritis. But the concept that gram-negative bacteria could be involved even in chronic arthritis is well-supported. Gram stains are more definitive in staphylococcal arthritis caused by gram-positive bacteria than in bacterial arthritis due to gram-negative bacteria. In the latter situation where gram-negative bacilli are the problem, Gram stains are helpful for 50% of the patients; they are only helpful for 25% of the patients, however, where gram-negative gonococci are the problem. In arthritis due to gram-positive Staphylococci. Gramstained smears are positive for 75% of the patients.

Oral budenosid is highly effective in the treatment of collagenous colitis: A randomized, double-blind, placebo-controlled, multicenter trial

2001 ◽  
Vol 120 (5) ◽  
pp. A40-A40 ◽  
Author(s):  
S MIEHLKE ◽  
P HEYMER ◽  
T OCHSENKUEHN ◽  
E BAESTLEIN ◽  
G YARIAN ◽  
...  
Keyword(s):  
Collagenous Colitis ◽  
Multicenter Trial ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Highly Effective
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