scholarly journals Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats

2015 ◽  
pp. 3651 ◽  
Author(s):  
Xiaomin Liu ◽  
Rui Hu ◽  
Hongwei Lian ◽  
Yang Liu ◽  
Jing Liu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Aldose Reductase ◽  
Diabetic Rats ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Dual Color ◽  
Associated Proteins

scholarly journals BIOCHEMICAL STUDIES OF HIPPOCAMPAL GENE EXPRESSION OF BRAIN-DERIVED NEUROTROPIC FACTOR AND TOLL-LIKE RECEPTOR-4 IN DIABETIC RATS EXPOSED TO CHRONIC STRESS: EFFECTS OF ANTIDEPRESSANT DRUGS

2018 ◽  
Vol 11 (1) ◽  
pp. 271
Author(s):  
Sawsan Aboul-fotouh ◽  
Doaa Mohamed Hassan ◽  
Mohamed Zaki Eldeen Habib ◽  
Ahmed Ibrahim Amin ◽  
Samar K. Kassim ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Antidepressant Drugs ◽  
Chronic Administration ◽  
Diabetic Rats ◽  
Toll Like Receptor ◽  
Bdnf Expression ◽  
Toll Like Receptor 4 ◽  
Inflammatory State ◽  
Neurotropic Factor ◽  
Factor Α

  Objective: Depression and diabetes are closely associated in a reciprocal manner, leading to significant morbidity and mortality with an evidence of a pro-inflammatory state underlying pathophysiology of both diseases. Unfortunately, little information is available about the effects of antidepressant drugs on hippocampal brain-derived neurotrophic factor (BDNF) and toll-like receptor-4 (TLR-4) expression in diabetes.Methods: We investigated the effect of chronic administration of fluoxetine (FLU) and imipramine (IMIP) on behavioral, metabolic, and inflammatory abnormalities in diabetic and non-diabetic rats exposed to chronic restraint stress (CRS).Results: Both diabetes and CRS induced depressive-like behavior which was more prominent in diabetic/depressed rats; this was reversed by chronic treatment with FLU and IMIP. Diabetic and non-diabetic rats exposed to CRS showed a significant increase in hippocampal expression of TLR-4 and pro-inflammatory cytokines alongside a decrease in BDNF expression. FLU and IMIP ameliorated these inflammatory abnormalities.Conclusion: Diabetes mellitus (DM) and chronic stress induced a depressive-like behavior associated with an increase in hippocampal expression of TLR-4, tumor necrosis factor-α, and interleukin-1ß with a significant correlation to decreased BDNF expression. FLU and IMIP showed comparable effects regards the improvement of depressive and inflammatory abnormalities associated with DM.

Blockade of Toll-Like Receptor 4 Attenuates Erectile Dysfunction in Diabetic Rats

2018 ◽  
Vol 15 (9) ◽  
pp. 1235-1245 ◽  
Author(s):  
Kenia Pedrosa Nunes ◽  
Amanda Almeida de Oliveira ◽  
Theodora Szasz ◽  
Vinicia Campana Biancardi ◽  
R. Clinton Webb
Keyword(s):  
Erectile Dysfunction ◽  
Diabetic Rats ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals MicroRNA-181a contributes to gastric hypersensitivity by targeting toll-like receptor 4 in diabetic rats

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S158-S159
Author(s):  
Hong-Hong Zhang ◽  
Ji Hu ◽  
Bing-Yu Zhang ◽  
Qian Sun ◽  
Yi-Lian Zhang ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals Toll-Like Receptor 2 Represses Nonpilus Adhesin-Induced Signaling in Acute Infections with the Pseudomonas aeruginosa pilA Mutant

2004 ◽  
Vol 72 (8) ◽  
pp. 4561-4569 ◽  
Author(s):  
Eva Lorenz ◽  
Diana C. Chemotti ◽  
Karen Vandal ◽  
Philippe A. Tessier
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Response ◽  
Lavage Fluid ◽  
Toll Like Receptor ◽  
Wild Type ◽  
Toll Like Receptor 4 ◽  
Acute Infections ◽  
Associated Proteins ◽  
Important Means ◽  
Toll Like Receptor 2

ABSTRACT Expression of pili and associated proteins is an important means of host invasion by bacterial pathogens. Recent evidence has suggested that the binding of Pseudomonas aeruginosa through nonpilus adhesins may also be important in respiratory diseases, since adhesins bind mucins. Using wild-type C57BL/6 and TLR2KO mice, we compared the induction levels of the host response to P. aeruginosa that either expressed pili or lacked pilus expression due to a mutation in the structural gene pilA. In C57BL/6 mice, deletion of pili led to a decreased immune response, evidenced by a lower secretion of cytokines and a lack of neutrophil chemotaxis. By contrast, the P. aeruginosa pilA mutant induced a hyperresponsive phenotype in TLR2KO mice. TLR2KO mice showed an increased number of neutrophils in lavage fluid compared to the levels seen when either mouse strain was exposed to wild-type P. aeruginosa. Further analysis indicated that the increased neutrophil influx was associated with an increased expression of calgranulins, possibly through an induction of Toll-like receptor 4 (TLR4) expression. The hyperresponsive phenotype of TLR2KO mice exposed to the P. aeruginosa pilA mutant was associated with TLR4 induction and indicated that nonpilus adhesin-induced signaling was repressed by TLR2 function and, if not blocked by the host, could induce airway hyperresponsiveness.

scholarly journals Diabetic Nephropathy Progression in type 1 Diabetes: Temporal Renal Angiopoietin-Like Protein 2-Toll-Like Receptor 4 and Role of Early Renal Angiotensin II Inhibition by Valsartan

2021 ◽  
Author(s):  
Mona K. Tawfik ◽  
mohammed keshawy ◽  
Samy Makary
Keyword(s):  
Blood Pressure ◽  
Diabetic Nephropathy ◽  
Angiotensin Ii ◽  
Kidney Injury ◽  
Diabetic Rats ◽  
Vehicle Group ◽  
Ang Ii ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Urine Albumin

Abstract Diabetic nephropathy (DN) is a consequence of diabetes mellitus (DM). DM is associated temporal changes in renal angiotensin II (ANG II) release and multiple mediators leading to DN. These changes were evaluated using early ANG II blocker valsartan as a DN renoprotective drug. Adult male Wister rats were divided into (i) vehicle group; (ii) valsartan received oral 30 mg/Kg/day; (iii) diabetic received single 50 mg/Kg intraperitoneal streptozotocin injection; (iv) renoprotection, valsartan treated-diabetic rats after 7 days from DM. Other group of diabetic animals assigned to receive late valsartan intervention from week 9 to 12 of DM. The renoprotective effect evaluated at 4th, 8th, 12th weeks. DN effects on urine albumin excretion, blood pressure and renal ANG II were measured. Urinary nephrin and kidney injury molecule-1 biomarkers, renal ANGPTL2, and toll-like receptor 4 (TLR 4) mRNA expression were tested. DN-initiated fibrotic markers integrin, α-smooth muscle expression and collagen IV and apoptotic protein caspase 3 were tested. DM induced changes starting from the 4th week. At 12th week, early valsartan intervention showed a significant reduction in ANG II, ANGPTL2 and TLR 4 expression and improvement in albuminuria, blood pressure, urinary biomarkers, fibrotic and apoptotic markers, more than the late intervention. Early inhibition of ANG II in diabetes is associated with decrease in ANGPTL2 and TLR 4 proteins and fibrotic changes. This observation helps in understanding DN pathophysiology and its therapeutic approaches.

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