scholarly journals Rescuing apoptotic neurons in Alzheimer’s disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin

2015 ◽  
pp. 2653 ◽  
Author(s):  
Yung-Chih Kuo ◽  
Ching-Chun Lin
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Nerve Growth

scholarly journals Alteration of binding properties and cytoskeletal attachment of nerve growth factor receptors in PC12 cells by wheat germ agglutinin.

1982 ◽  
Vol 94 (3) ◽  
pp. 710-717 ◽  
Author(s):  
R D Vale ◽  
E M Shooter
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Binding Properties ◽  
Nerve Growth ◽  
Total Binding ◽  
Ngf Receptor ◽  
Triton X

Incubation of PC12 cells preloaded with 125I-nerve growth factor (NGF) reveals rapidly and slowly dissociating binding components indicative of a heterogeneous population of receptors. If the cells are previously exposed to wheat germ agglutinin (WGA) for 30 min, NGF now binds to an apparently homogeneous receptor population which exhibit slow dissociation kinetics. Total binding is also reduced by 50%. If WGA is added subsequent to 125I-NGF, total binding is not diminished, but rapidly dissociating receptors occupied with NGF are all converted to the slowly dissociating form. This conversion of receptors occurs rapidly, reaching completion within 2 min at 37 degrees or 4 degrees C, and is unaffected by metabolic energy poisons, suggesting that WGA-induced slowly dissociating receptors are not the product of internalization. The effects of the lectin are blocked by the sugar N-acetyl-D-glucosamine, and the lectin-induced slowly dissociating receptors are converted back to rapidly dissociating receptors by addition of this same sugar. WGA also affects the association of the NGF receptor with the Triton X-100 cytoskeleton. Greater than 90% of bound 125I-NGF becomes associated with Triton X-100 insoluble cytoskeletons in the presence of the lectin, compared with less than 20% before lectin addition. Cytoskeleton association of the NGF receptor by WGA shows similar kinetics as the conversion of rapidly to slowly dissociating receptors. This interaction may be involved in the alteration of NGF-receptor binding properties produced by this lectin.

scholarly journals Wheat germ agglutinin blocks the biological effects of nerve growth factor.

1985 ◽  
Vol 101 (5) ◽  
pp. 1690-1694 ◽  
Author(s):  
G E Landreth ◽  
L K Williams ◽  
C McCutchen
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Surface ◽  
Pc12 Cells ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Biological Effects ◽  
Receptor Interaction ◽  
Cytoskeletal Protein ◽  
Ngf Receptor

The binding of nerve growth factor (NGF) to specific cell surface receptors initiates a variety of effects that lead to the morphological and biochemical differentiation of clonal pheochromocytoma, PC12, cells. The lectin wheat germ agglutinin (WGA) alters the characteristics of NGF-receptor interaction. We have found that treatment of PC12 cells with WGA dramatically and reversibly inhibits the ability of NGF to elicit three distinct biological effects characteristic of NGF action. Two of these events, the rapid ruffling of cell-surface membranes and the stimulation of the phosphorylation of a 250-kD cytoskeletal protein in situ, occur rapidly and are an immediate consequence of receptor occupancy. Both of these effects are blocked by pretreatment of the cells with WGA. WGA was also found to inhibit the NGF-stimulated regeneration of neurites that occurs over 1-2 d. Both the WGA inhibition of neurite outgrowth and the phosphorylation of the 250-kD cytoskeletal protein were reversed upon addition of the specific sugar N-acetylglucosamine. These data demonstrate that the WGA-induced changes in the NGF-receptor interaction reflect important alterations in the ability of the receptor to transmit biological signals, resulting in the abrogation of the biological effects of NGF on these cells.

Screening Natural Products for Effects on Nerve Growth Factor (NGF) Stimulated Neuritogenesis and Neuritic Outgrowth

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
ND Chaurasiya ◽  
R Sahu ◽  
V Samoylenko ◽  
M Ilias ◽  
LA Walker ◽  
...  
Keyword(s):  
Natural Products ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Neuritic Outgrowth
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