scholarly journals In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma

2015 ◽  
pp. 1585 ◽  
Author(s):  
André Chwalibog ◽  
Slawomir Jaworski ◽  
Ewa Sawosz ◽  
Marta Kutwin ◽  
Mateusz Wierzbicki ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Reduced Graphene ◽  
In Vivo Effects

scholarly journals Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sajad Bahrami ◽  
Nafiseh Baheiraei ◽  
Mostafa Shahrezaee
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Cranial Bone ◽  
Drying Methods ◽  
Porous Scaffolds ◽  
Alizarin Red ◽  
Reduced Graphene ◽  
Coated Collagen

AbstractA variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.

Heparin-reduced graphene oxide nanocomposites for curcumin delivery: in vitro, in vivo and molecular dynamics simulation study

2019 ◽  
Vol 7 (3) ◽  
pp. 1011-1027 ◽  
Author(s):  
Xiaoqun Shi ◽  
Yang Wang ◽  
Haiyan Sun ◽  
Yujuan Chen ◽  
Xingzhen Zhang ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Graphene Oxide ◽  
Molecular Dynamics Simulation ◽  
Reduced Graphene Oxide ◽  
Simulation Study ◽  
Dynamics Simulation ◽  
Reduced Graphene

We fabricated novel rGO-based nanocomposites and analyzed their interaction with drug and proteins via a molecular dynamics study.

CdSe- Reduced graphene oxide nanocomposite toxicity alleviation via V2O5 shell formation over CdSe core: in vivo and in vitro studies

2020 ◽  
Vol 31 (41) ◽  
pp. 415101
Author(s):  
Ashwani Kumar Singh ◽  
Amar Nath Yadav ◽  
Saurabh Srivastav ◽  
Rishi Kumar Jaiswal ◽  
Amit Srivastava ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
In Vitro Studies ◽  
Shell Formation ◽  
Cdse Core ◽  
Reduced Graphene

scholarly journals Plasmonic photothermal cancer therapy with gold nanorods/reduced graphene oxide core/shell nanocomposites

RSC Advances ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 1600-1610 ◽  
Author(s):  
Kostiantyn Turcheniuk ◽  
Tetiana Dumych ◽  
Rostyslav Bilyy ◽  
Volodymyr Turcheniuk ◽  
Julie Bouckaert ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cancer Therapy ◽  
Reduced Graphene Oxide ◽  
Photon Energy ◽  
Gold Nanorods ◽  
Core Shell ◽  
Efficient Conversion ◽  
Reduced Graphene

Gold nanorods (Au NRs) are known for their efficient conversion of photon energy into heat, resulting in hyperthermia and suppression of tumor growths in vitro and in vivo.

scholarly journals Gelatin reduced Graphene Oxide Nanosheets as Kartogenin Nanocarrier Induces Rat ADSCs Chondrogenic Differentiation Combining with Autophagy Modification

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1053
Author(s):  
Delong Jiao ◽  
Jing Wang ◽  
Wenting Yu ◽  
Ning Zhang ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Chondrogenic Differentiation ◽  
Drug Loading ◽  
Pcr Analysis ◽  
Promoting Effect ◽  
Reduced Graphene

Biocompatible reduced graphene oxide (rGO) could deliver drugs for synergistically stimulating stem cells directed differentiation with influences on specific cellular activities. Here, we prepared a biodegradable gelatin reduced graphene oxide (rGO@Ge) to evaluate its functions in promoting rat adipose derived mesenchymal stem cells (ADSCs) chondrogenic differentiation through delivering kartogenin (KGN) into the stem cell efficiently. The optimum KGN concentration (approximately 1 μM) that promoted the proliferation and chondrogenic differentiation of ADSCs was clarified by a series of experiments, including immunofluorescent (IF) staining (Sox-9, Col II), alcian blue (Ab) staining, toluidine blue (Tb) staining and real-time quantitative PCR analysis of the chondrogenic markers. Meanwhile, the biocompatibility of rGO@Ge was evaluated to clearly define the nonhazardous concentration range, and the drug loading and releasing properties of rGO@Ge were tested with KGN for its further application in inducing ADSCs chondrogenic differentiation. Furthermore, the mechanism of rGO@Ge entering ADSCs was investigated by the different inhibitors that are involved in the endocytosis of the nanocarrier, and the degradation of the rGO@Ge in ADSCs was observed by transmission electron microscopy (TEM). The synergistic promoting effect of rGO@Ge nanocarrier on ADSCs chondrogenesis with KGN was also studied by the IF, Ab, Tb stainings and PCR analysis of the chondrogenic markers. Finally, the intracellular Reactive Oxygen Species (ROS) and autophagy induced by KGN/rGO@Ge complex composites were tested in details for clarification on the correlation between the autophagy and chondrogenesis in ADSCs induced by rGO@Ge. All the results show that rGO@Ge as a biocompatible nanocarrier can deliver KGN into ADSCs for exerting a pro-chondrogenic effect and assist the drug to promote ADSCs chondrogenesis synergistically through modification of the autophagy in vitro, which promised its further application in repairing cartilage defect in vivo.

Near-infrared emitting graphene quantum dots synthesized from reduced graphene oxide for in vitro/in vivo/ex vivo bioimaging applications

2D Materials ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 035013
Author(s):  
Md Tanvir Hasan ◽  
Bong Han Lee ◽  
Ching-Wei Lin ◽  
Ainsley McDonald-Boyer ◽  
Roberto Gonzalez-Rodriguez ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Near Infrared ◽  
Ex Vivo ◽  
Graphene Quantum Dots ◽  
Reduced Graphene

scholarly journals Enhanced osseointegration of dental implants with reduced graphene oxide coating

2022 ◽  
Author(s):  
Yong Cheol Shin ◽  
Ji-Hyeon Bae ◽  
Jong Ho Lee ◽  
Iruthayapandi Selestin Raja ◽  
Moon Sung Kang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Cell Attachment ◽  
Pure Titanium ◽  
Bone Morphogenetic Protein 2 ◽  
In Vivo Studies ◽  
Matrix Mineralization ◽  
Reduced Graphene

Abstract Background: The implants of pure titanium (Ti) and its alloys can lead to implant failure because of their poor interaction with bone-associated cells during bone regeneration. Surface modification over implants has achieved successful implants for enhanced osseointegration.Methods: Herein, we prepared sandblasted, large-grit, and acid-etched (SLA) Ti (ST) implants with different surface modifications [i.e., reduced graphene oxide (rGO) and recombinant human bone morphogenetic protein-2 (rhBMP-2)] and investigated their dental tissue regenerating ability in animal models. We performed comparative studies in surface property, in vitro cellular behaviors, and in vivo osseointegration activity among different groups, including ST (control), rhBMP-2-immobilized ST (BI-ST), rhBMP-2-treated ST (BT-ST), and rGO-coated ST (R-ST).Results: Spectroscopic, diffractometric, and microscopic analyses confirmed that rGO was coated well around the surfaces of Ti discs (for cell study) and implant fixtures (for animal study). Furthermore, in vitro and in vivo studies revealed that the R-ST group showed significantly better effects in cell attachment and proliferation, alkaline phosphatase activity, matrix mineralization, and osseointegration than the control (ST), BI-ST, and BT-ST groups.Conclusion: Hence, we suggest that the rGO-coated Ti can be a promising candidate for the application to dental or even orthopedic implants due to its ability to accelerate the healing rate with the high potential of osseointegration.

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